Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
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Journal ArticleDOI
Affinity for the cognate monoclonal antibody of synthetic peptides derived from selection by phage display
Gaëlle Ferrières,Sylvie Villard,Martine Pugnière,Jean-Claude Mani,Isabelle Navarro-Teulon,F Rharbaoui,Daniel Laune,Erwann Loret,Bernard Pau,Claude Granier +9 more
TL;DR: The affinity of a given peptide strongly depends on its capacity to maintain the antigenically reactive structure it has on the phage, implying that it is impossible to predict whether high- or low-affinity peptides will be obtained from phage display.
Journal Article
Disulfide-constrained peptides that bind to the extracellular portion of the prostate-specific membrane antigen
Shawn E. Lupold,Ronald Rodriguez +1 more
TL;DR: In this paper, two separate phage display peptide strategies were applied, in parallel, to purified PSMA protein bound to two separate substrates, and the design allowed for stringent cross-selections, where phage populations from one selection condition could be applied to the alternative substrate.
Journal ArticleDOI
Anti-free prostate-specific antigen monoclonal antibody epitopes defined by mimotopes and molecular modeling.
Sandrine Michel,Gilbert Deléage,Jean-Philippe Charrier,Jacques Passagot,Nicole Battail-Poirot,Geneviève Sibaï,Michel Jolivet,Colette Jolivet-Reynaud +7 more
TL;DR: These epitope localizations are in agreement with the ability of both mAbs to inhibit enzymatic activity and ACT fixation, and could bring information for the generation of clinically relevant PSA assays.
Journal ArticleDOI
In Silico Generation of Peptides by Replica Exchange Monte Carlo: Docking-Based Optimization of Maltose-Binding-Protein Ligands
Anna Russo,Pasqualina Liana Scognamiglio,Rolando P. Hong Enriquez,Carlo Santambrogio,Rita Grandori,Daniela Marasco,Antonio Giordano,Antonio Giordano,Giacinto Scoles,Giacinto Scoles,Sara Fortuna +10 more
TL;DR: Ex-novo peptides targeting the maltose site of the Maltose Binding Protein, the prototypical system for the study of protein ligand recognition are developed using a Monte Carlo based protocol to computationally evolve a set of octapeptides starting from a polialanine sequence.
Journal ArticleDOI
Epitope mapping of monoclonal antibodies to keratin 19 using keratin fragments, synthetic peptides and phage peptide libraries
TL;DR: A group of monoclonal antibodies reacting with the intermediate filament protein keratin 19 were studied using different approaches to define the structure and localization of their epitopes to generate tools for monitoring processing and folding in keratin intermediate filaments.
References
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