Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
Citations
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Journal ArticleDOI
Bacteriophage display and discovery of peptide leads for drug development
TL;DR: This review, though not comprehensive with respect to the explosive volume of phage display work over the last few years, focuses on recent developments in phage-displayed peptide technology.
Journal ArticleDOI
Mono‐ and Bivalent Antibody Fragments Produced in Escherichia coli: Engineering, Folding and Antigen Binding
TL;DR: The technology to produce monoclonal antibodies has led to the determination of an enormous number of antibody sequences, mostly from the cloned DNA sequence, crucial for defining consensus sequences, which allowed rapid cloning of antibody genes by the polymerase chain reaction (PCR), thereby generating even more sequence information.
Journal ArticleDOI
One-bead–one-structure combinatorial libraries
Michal Lebl,Viktor Krchnak,Nikolai Sepetov,Bruce Seligmann,Peter Strop,Stephen Felder,Kit S. Lam +6 more
TL;DR: Direct methods of structure identification as well as indirect “coding” methods facilitating the synthesis and screening of nonpeptide libraries are discussed, and the ability to use combinatorial libraries to optimize initially discovered leads is illustrated with examples using peptide libraries.
Patent
Human growth hormone variants
Brian C. Cunningham,Henry B. Lowman,James A. Wells,Ross G. Clark,Kenneth Charles Olson,Germaine Fuh +5 more
TL;DR: In this article, human growth hormone variants are disclosed having enhanced affinity for the growth hormone receptor and conjugated to one or more chemical groups, such as poly(ethylene glycol), which is believed to prolong the in vivo half-life of the variants.
Journal ArticleDOI
Screening of a Library of Phage-displayed Peptides Identifies Human Bcl-2 as a Taxol-binding Protein
Diane J. Rodi,Robert W. Janes,Robert W. Janes,Hitesh J. Sanganee,Robert A. Holton,Bonnie A. Wallace,Lee Makowski +6 more
TL;DR: It is demonstrated that peptides displayed on the surface of bacteriophage particles can mimic the ligand-binding properties of disordered regions of proteins.
References
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