Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
Citations
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Journal ArticleDOI
Design of synthetic gene libraries encoding random sequence proteins with desired ensemble characteristics.
TL;DR: Semirandom DNA, synthesized with a designed, three‐residue repeat pattern, can encode libraries of very high diversity and represents an important tool for the construction of random polypeptide libraries.
Journal ArticleDOI
Creation of libraries with long orfs by polymerization of a microgene
TL;DR: A novel method for constructing pools of DNA sequences that encode large proteins with molecular diversity by polymerization of microgene polymers, which have long ORFs whose products have a repetitious nature that could facilitate the formation of higher structures of translated products.
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Applied molecular evolution
TL;DR: The fact, that second-rank antibodies can serve as vaccines for bacterial pathogens whose protective antigen is a carbohydrate, shows that proteins can mimic the shape of a chemically different family.
Journal ArticleDOI
Peptide Conjugation of 2′-O-methyl Phosphorothioate Antisense Oligonucleotides Enhances Cardiac Uptake and Exon Skipping in mdx Mice
Silvana M.G. Jirka,Hans Heemskerk,Christa L Tanganyika-de Winter,Daan Muilwijk,Kar Him Pang,Peter C de Visser,Anneke A.M. Janson,Tatyana G Karnaoukh,Rick Vermue,Peter A C 't Hoen,Judith C.T. van Deutekom,Begoña Aguilera,Annemieke Aartsma-Rus +12 more
TL;DR: A 7-mer phage display peptide library is screened to identify muscle and heart homing peptides in vivo in the mdx mouse model and a promising candidate peptide capable of binding muscle cells in vivo is found.
Journal ArticleDOI
Immunology of scorpion toxins and perspectives for generation of anti-venom vaccines.
TL;DR: A novel approach based on mimotopes selected from phage-displayed random peptide libraries show potential to impel further progress of toxin immunological studies and to provide putative vaccine resources.
References
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Journal ArticleDOI
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