Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
Citations
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Journal ArticleDOI
Ricin detection using phage displayed single domain antibodies.
Ellen R. Goldman,Jinny L. Liu,Rachael D. Bernstein,Marla D. Swain,Stanley Q. Mitchell,George M. Anderson +5 more
TL;DR: The phage-displayed single domain antibodies (sdAb) led to five to ten fold better detection of ricin in both the ELISA and Luminex assays, resulting in limits of detection of 1 ng/mL and 64 pg/mL respectively.
Journal ArticleDOI
Somatostatin displayed on filamentous phage as a receptor-specific agonist.
Mat Rousch,Jan T. Lutgerink,James E. Coote,Adriaan P. de Bruïne,Jan-Willem Arends,Hennie R. Hoogenboom +5 more
TL;DR: Binding of somatostatin displaying phage to sst2 on a reporter cell line, in which binding of natural ligand reduces secretion of alkaline phosphatase (via a cyclic AMP responsive element sensitive promoter), proved that the phage particles act as receptor‐specific agonists.
Journal ArticleDOI
Combinatorial chemistry in the discovery and development of drugs.
TL;DR: A comprehensive review of the tactics and strategies that are available to the drug discovery process using combinatorial techniques.
Journal ArticleDOI
Isolation of peptides that mimic epitopes on a malarial antigen from random peptide libraries displayed on phage.
TL;DR: A monoclonal antibody against a malarial antigen can select common mimotopes from different random peptide libraries, and the envisage many uses for this technology in malaria research.
Patent
Multimeric peptide conjugates and uses thereof
TL;DR: In this paper, the authors proposed a method to use multimeric (e.g., dimeric, trimeric) forms of peptide vectors that are capable of crossing the blood-brain barrier (BBB) or efficiently entering particular cell types.
References
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Journal ArticleDOI
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