Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
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Patent
Methods and compositions for polypeptide engineering
TL;DR: Methods for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection, are provided in this paper, and compositions produced by these methods are also disclosed.
Journal ArticleDOI
Phage display technology: clinical applications and recent innovations.
TL;DR: This review serves as an introduction to phage display, antibody engineering, the development of phage-displayed peptides and antibody fragments into viable diagnostic reagents, and recent trends in display technology.
Patent
Matrices with memories and uses thereof
Michael P. Nova,Zahra Parandoosh,Andrew E. Senyei,Xiao-Yi Xiao,Gary S. David,Yozo Satoda,Chanfeng Zhao,Hanan Potash +7 more
TL;DR: In this paper, a matrix with memories (MVM) is defined, which is a combination of matrix materials, memories, and linked molecules and biological materials that are encoded with an optically readable code.
Patent
Selection of proteins using rna-protein fusions
TL;DR: In this article, RNA-protein fusion production methods which involve a high salt post-translational incubation step are described, and the authors describe a method for RNA-Protein fusion.
Journal ArticleDOI
Fingerprinting the circulating repertoire of antibodies from cancer patients
Paul J. Mintz,Jeri Kim,Kim Anh Do,Xuemei Wang,Ralph Zinner,Massimo Cristofanilli,Marco A. Arap,Waun Ki Hong,Patricia Troncoso,Christopher J. Logothetis,Renata Pasqualini,Wadih Arap +11 more
TL;DR: A consensus motif, NXS/TDKS/T, that bound selectively to circulating antibodies from cancer patients over control antibodies from blood donors was identified and validated by showing that positive serum reactivity to the peptide was specifically linked to disease progression and to shorter survival in a large patient population.
References
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