Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
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Notch and Wingless Regulate Expression of Cuticle Patterning Genes
TL;DR: The results suggest that the strong genetic interactions observed between Notch and wingless genes during development of Drosophila is at least partly due to regulation of expression of cuticle patterning genes by Wingless and the two forms of Notch.
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Combinatorial peptide libraries in drug design: lessons from venomous cone snails.
TL;DR: The venoms of cone snails are a new natural resource of peptides that may have a pharmaceutical potential equivalent to those from traditional sources, particularly for developing drugs that target cell-surface receptors or ion channels.
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Proteomics and diagnostics: Let's Get Specific, again.
TL;DR: This paper explores some possible ways to increase the content of proteomic analysis by improving the evaluation of reagent specificity.
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Modified phage peptide libraries as a tool to study specificity of phosphorylation and recognition of tyrosine containing peptides
Luciana Dente,Costantino Vetriani,Adriana Zucconi,Giuliana Pelicci,Luisa Lanfrancone,Pier Giuseppe Pelicci,Gianni Cesareni +6 more
TL;DR: The approach permits us to reveal an "extended" consensus indicating that residues that do not seem to influence binding in natural peptides can make productive contacts, at least in linear peptides.
Journal ArticleDOI
Defining rules for the peptide-MHC class II interaction
TL;DR: The presence of conserved and allele-specific anchors appears to explain the promiscuity and the specificity of peptide recognition by DR molecules.
References
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Journal ArticleDOI
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