Journal ArticleDOI
Searching for Peptide Ligands With an Epitope Library
Jamie K. Scott,George P. Smith +1 more
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TLDR
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library".Abstract:
Tens of millions of short peptides can be easily surveyed for tight binding to an antibody, receptor or other binding protein using an "epitope library." The library is a vast mixture of filamentous phage clones, each displaying one peptide sequence on the virion surface. The survey is accomplished by using the binding protein to affinity-purify phage that display tight-binding peptides and propagating the purified phage in Escherichia coli. The amino acid sequences of the peptides displayed on the phage are then determined by sequencing the corresponding coding region in the viral DNA's. Potential applications of the epitope library include investigation of the specificity of antibodies and discovery of mimetic drug candidates.read more
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Journal ArticleDOI
Artificial Multienzyme Scaffolds: Pursuing in Vitro Substrate Channeling with an Overview of Current Progress
Gregory A. Ellis,William P. Klein,William P. Klein,Guillermo Lasarte-Aragonés,Guillermo Lasarte-Aragonés,Meghna Thakur,Meghna Thakur,Scott A. Walper,Igor L. Medintz +8 more
TL;DR: Artificial multienzyme scaffolds are being developed for in vitro cascaded biocatalytic activity and, in particular, accessing substrate channeling, and progress over the past five years is covered.
Patent
Histone deacetylases, and uses related thereto
TL;DR: In this paper, the discovery that proteins encoded by a family of genes, termed here HDx-related genes, which are involved in the control of chromatin structure and thus in transcription and translation was made.
Journal ArticleDOI
A Unique Substrate Binding Mode Discriminates Membrane Type-1 Matrix Metalloproteinase from Other Matrix Metalloproteinases
Steven J. Kridel,Hisako Sawai,Boris I. Ratnikov,Emily I. Chen,Weizhong Li,Adam Godzik,Alex Y. Strongin,Jeffrey W. Smith +7 more
TL;DR: The study provides a structural basis for selective and non-selective substrate recognition by MT1-MMP, and indicates that the selective substrates adopt a linear conformation that extends along the entire catalytic pocket of MT1, whereas non- selective substrates are kinked at the conserved P3 Pro residue.
Journal ArticleDOI
Recent advances in phage display.
TL;DR: The selection of peptides and proteins from libraries expressed on the surface of filamentous phage is becoming an important tool in biotechnology to examine the specificities of natural enzymes as well as to evolve novel enzymes de novo.
Journal ArticleDOI
Phage display technology: applications and innovations
TL;DR: The applications, recent innovations, and future directions of phage display technology are reviewed.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
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Journal ArticleDOI
Rapid and efficient site-specific mutagenesis without phenotypic selection.
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Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.