Single-Cell Exome Sequencing and Monoclonal Evolution of a JAK2-Negative Myeloproliferative Neoplasm
Yong Hou,Luting Song,Ping Zhu,Bo Zhang,Ye Tao,Xun Xu,Fuqiang Li,Kui Wu,Jie Liang,Di Shao,Hanjie Wu,Xiaofei Ye,Chen Ye,Renhua Wu,Min Jian,Yan Chen,Wei Xie,Ruren Zhang,Lei Chen,Xin Liu,Xiaotian Yao,Hancheng Zheng,Chang Yu,Qibin Li,Zhuolin Gong,Mao Mao,Xu Yang,Lin Yang,Jingxiang Li,Wen Wang,Zuhong Lu,Ning Gu,Goodman Laurie,Lars Bolund,Karsten Kristiansen,Jian Wang,Huanming Yang,Yingrui Li,Xiuqing Zhang,Jun Wang +39 more
TLDR
A method for analyzing the cancer genome at a single-cell nucleotide level is described and essential thrombocythemia-related candidate mutations such as SESN2 and NTRK1 are identified, which may be involved in neoplasm progression.About:
This article is published in Cell.The article was published on 2012-03-02 and is currently open access. It has received 524 citations till now. The article focuses on the topics: Exome sequencing & Single cell sequencing.read more
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Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples
Kristian Cibulskis,Michael S. Lawrence,Scott L. Carter,Andrey Sivachenko,David M. Jaffe,Carrie Sougnez,Stacey Gabriel,Matthew Meyerson,Matthew Meyerson,Eric S. Lander,Eric S. Lander,Eric S. Lander,Gad Getz,Gad Getz +13 more
TL;DR: The MuTect algorithm for calling somatic point mutations enables subclonal analysis of the whole-genome or whole-exome sequencing data being generated in large-scale cancer genomics projects as discussed by the authors.
Journal ArticleDOI
Full-length RNA-seq from single cells using Smart-seq2
Simone Picelli,Omid R. Faridani,Åsa K. Björklund,Gösta Winberg,Sven Sagasser,Rickard Sandberg +5 more
TL;DR: In this article, the authors presented a detailed protocol for Smart-seq2 that allows the generation of full-length cDNA and sequencing libraries by using standard reagents, and the entire protocol takes ∼2 d from cell picking to having a final library ready for sequencing; sequencing will require an additional 1-3 d depending on the strategy and sequencer.
Journal Article
Patterns of Somatic Mutation in Human Cancer Genomes
TL;DR: In this paper, the coding exons of the family of 518 protein kinases were sequenced in 210 cancers of diverse histological types to explore the nature of the information that will be derived from cancer genome sequencing.
Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples
Kristian Cibulskis,Andrey Sivachenko,David B. Jaffe,Carrie Sougnez,Stacey Gabriel,Matthew Meyerson,Gad Getz,Eric S. Lander,Michael S. Lawrence,Scott L. Carter +9 more
TL;DR: MuTect is presented, a method that applies a Bayesian classifier to detect somatic mutations with very low allele fractions, requiring only a few supporting reads, followed by carefully tuned filters that ensure high specificity.
Full-length RNA-seq from single cells using
TL;DR: A detailed protocol is presented for Smart-seq2 that allows the generation of full-length cDNA and sequencing libraries by using standard reagents and the lack of strand specificity and the inability to detect nonpolyadenylated (polyA−) RNA.
References
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Accurate whole human genome sequencing using reversible terminator chemistry
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TL;DR: An approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost is reported, effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.
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