Single-Molecule Fluorescence Reveals the Unwinding Stepping Mechanism of Replicative Helicase
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TLDR
A coupling ratio of 1:1 between base pairs unwound and dTTP hydrolysis is suggested, which further support the concept that nucleic acid motors can have a hierarchy of different-sized steps or can accumulate elastic energy before transitioning to a subsequent phase.About:
This article is published in Cell Reports.The article was published on 2014-03-27 and is currently open access. It has received 54 citations till now. The article focuses on the topics: Helicase.read more
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Early Time Solvation Dynamics Probed by Spectrally Resolved Degenerate Pump-Probe Spectroscopy.
TL;DR: It is shown that major differences in solvation by ethanol and ethylene glycol is contributed by early time (<1 ps) dynamics and time-resolved emission spectra can be directly used for constructing relaxation correlation function, obviating spectral reconstruction and estimation of time-zero spectrum in non-polar solvents.
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A mechanism for the extension and unfolding of parallel telomeric G-quadruplexes by human telomerase at single-molecule resolution
Bishnu P. Paudel,Aaron L. Moye,Hala Abou Assi,Roberto El-Khoury,Scott B. Cohen,Monica L. Birrento,Siritron Samosorn,Kamthorn Intharapichai,Christopher G. Tomlinson,Marie-Paule Teulade-Fichou,Marie-Paule Teulade-Fichou,Carlos González,Jennifer L. Beck,Masad J. Damha,Antoine M. van Oijen,Tracy M. Bryan +15 more
TL;DR: Surprisingly, parallel G4 stabilization with either small molecule ligands or by chemical modification does not always inhibit G4 unfolding and extension by telomerase, revealing that telomersase is a parallel G-quadruplex resolvase.
Journal ArticleDOI
Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase.
Sean P. Carney,Wen Ma,Wen Ma,Kevin D. Whitley,Kevin D. Whitley,Haifeng Jia,Timothy M. Lohman,Zaida Luthey-Schulten,Yann R. Chemla +8 more
TL;DR: In this paper, the authors used optical tweezers to measure directly the stepwise translocation of UVRD along a DNA hairpin, and propose a mechanism in which UvrD moves one base pair at a time, but sequesters the nascent single strands, releasing them after a variable number of ATP hydrolysis cycles.
Journal ArticleDOI
Quantitative protein detection using single molecule imaging enzyme-linked immunosorbent assay (iELISA).
TL;DR: Considering its high accuracy in target protein detection with simple procedures and fast speed, it is believed single molecule iELISA can be potentially adopted in fast trace protein detection.
Journal ArticleDOI
Processivity, Velocity, and Universal Characteristics of Nucleic Acid Unwinding by Helicases.
TL;DR: This work develops a mathematical framework and derive analytic expressions for the velocity and run length of a general model of finitely processive helicases, the two most commonly measured experimental quantities, and generates fundamental insights into the force response of helicases.
References
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