Single-Molecule Fluorescence Reveals the Unwinding Stepping Mechanism of Replicative Helicase
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TLDR
A coupling ratio of 1:1 between base pairs unwound and dTTP hydrolysis is suggested, which further support the concept that nucleic acid motors can have a hierarchy of different-sized steps or can accumulate elastic energy before transitioning to a subsequent phase.About:
This article is published in Cell Reports.The article was published on 2014-03-27 and is currently open access. It has received 54 citations till now. The article focuses on the topics: Helicase.read more
Citations
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Journal ArticleDOI
Open-ringed structure of the Cdt1-Mcm2-7 complex as a precursor of the MCM double hexamer
Yuanliang Zhai,Erchao Cheng,Hao Wu,Ningning Li,Philip Y. K. Yung,Ning Gao,Bik Kwoon Tye,Bik Kwoon Tye +7 more
TL;DR: The intrinsic coiled structures of the precursors provide insights into the DH formation, and suggest a spring-action model for the MCM during the initial origin melting and the subsequent DNA unwinding.
Journal ArticleDOI
Simultaneous Real-Time Imaging of Leading and Lagging Strand Synthesis Reveals the Coordination Dynamics of Single Replisomes
Karl E. Duderstadt,Karl E. Duderstadt,Karl E. Duderstadt,Hylkje Geertsema,Sarah A. Stratmann,Christiaan M. Punter,Arkadiusz W. Kulczyk,Charles C. Richardson,Antoine M. van Oijen,Antoine M. van Oijen +9 more
TL;DR: It is shown that loops in the lagging strand predominantly occur during priming and only infrequently support subsequent Okazaki-fragment synthesis, reconciling divergent models for the regulation of primer synthesis and revealing an underlying plasticity in replisome operation.
Journal ArticleDOI
Single-Molecule Analysis and Engineering of DNA Motors
TL;DR: Techniques such as mutagenesis, chemical modifications, and optogenetics that have been used to re-engineer existing molecular motors to have, for instance, altered speed, processivity, or functionality are reviewed.
Journal ArticleDOI
Mechanical Operation and Intersubunit Coordination of Ring-Shaped Molecular Motors: Insights from Single-Molecule Studies
TL;DR: The various mechanisms by which ring motors convert chemical energy to mechanical force or torque and coordinate the activities of individual subunits that constitute the ring are described.
Journal ArticleDOI
The mechanism of DNA unwinding by the eukaryotic replicative helicase.
TL;DR: A mechanism in which purified recombinant Drosophila melanogaster CMG couples ATP hydrolysis to unwinding by acting as a lazy Brownian ratchet is proposed, providing quantitative understanding of the central process in eukaryotic DNA replication.
References
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Spatial regulation of RhoA activity during pancreatic cancer cell invasion driven by mutant p53
Paul Timpson,Ewan J. McGhee,Jennifer P. Morton,Alex von Kriegsheim,Juliane P. Schwarz,Saadia A. Karim,Brendan Doyle,Jean A. Quinn,Neil O. Carragher,Mike Edward,Michael F. Olson,Margaret C. Frame,Valerie G. Brunton,Owen J. Sansom,Kurt I. Anderson +14 more
TL;DR: Quantitative in vivo fluorescence lifetime imaging illustrated that RhoA is not only necessary for invasion, but also that subcellular spatial regulation of RHoA activity, as opposed to its global activity, is likely to govern invasion efficiency in vivo.
Journal ArticleDOI
Forward and Reverse Motion of Single RecBCD Molecules on DNA
TL;DR: Observations show that RecBCD-DNA complexes can exist in multiple, functionally distinct states that persist for many catalytic turnovers: such states may help tune enzyme activity for various biological functions.
Journal ArticleDOI
Real-time observation of bacteriophage T4 gp41 helicase reveals an unwinding mechanism
TL;DR: Together, the results suggest that important regulations occur within the replisome to achieve rapid and processive replication and that this weak helicase cannot efficiently unwind the T4 genome alone.
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χ-Sequence recognition and DNA translocation by single RecBCD helicase/nuclease molecules
Kathleen M. Dohoney,Jeff Gelles +1 more
TL;DR: Observed translocation of single RecBCD molecules is unidirectional, with each molecule moving at a constant velocity corresponding to the population-average DNA unwinding rate, placing strong constraints on possible movement mechanisms.
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Single-base pair unwinding and asynchronous RNA release by the hepatitis C virus NS3 helicase.
TL;DR: Asynchronous release of nascent nucleotides rationalizes various observations of its dsNA unwinding and may be used to coordinate the translocation speed of NS3 along the RNA during viral replication.