Structure of the Fungal Beta-Glucan-Binding Immune Receptor Dectin-1: Implications for Function.
James Brown,Christopher A. O’Callaghan,Andrew S J Marshall,Robert J.C. Gilbert,Christian Siebold,Siamon Gordon,Gordon D. Brown,E Y Jones +7 more
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TLDR
Several dectin‐1 crystal structures are reported, including a high‐resolution structure and a 2.8 Å resolution structure in which a short soaked natural β‐glucan is trapped in the crystal lattice, which suggest potential mechanisms of defense against fungal pathogens.Abstract:
The murine molecule dectin-1 (known as the β-glucan receptor in humans) is an immune cell surface receptor implicated in the immunological defense against fungal pathogens. Sequence analysis has indicated that the dectin-1 extracellular domain is a C-type lectin-like domain, and functional studies have established that it binds fungal β-glucans. We report several dectin-1 crystal structures, including a high-resolution structure and a 2.8 A resolution structure in which a short soaked natural β-glucan is trapped in the crystal lattice. In vitro characterization of dectin-1 in the presence of its natural ligand indicates higher-order complex formation between dectin-1 and β-glucans. These combined structural and biophysical data considerably extend the current knowledge of dectin-1 structure and function, and suggest potential mechanisms of defense against fungal pathogens.read more
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Human Dectin-1 is O-glycosylated and serves as a ligand for C-type lectin receptor CLEC-2
TL;DR: In this article , a sialylated core 1 glycan attached to the EDxxT motif of human Dectin-1 provided a ligand moiety for CLEC-2, another lectin receptor expressed on platelets.
Book ChapterDOI
Dectin-1 (CLEC7A, BGR, CLECSF12)
TL;DR: The role of Dectin-1 is described in antifungal immunity and in immunity to other pathogens, as well as the intracellular signalling pathways and cellular responses that this receptor can induce.
Journal ArticleDOI
Synthesis and evaluation of 1,5-dithialaminaribiose and -triose tetravalent constructs.
TL;DR: In this article , the synthesis of tetravalent glucoclusters containing 1,5-dithia mimetics of laminaribiose and triose was reported.
Posted ContentDOI
Dectin-1 Molecular Aggregation and Signaling is Sensitive to β-Glucan Structure and Glucan Exposure on Candida albicans Cell Walls
Eduardo U. Anaya,Akram Etemadi Amin,Michael J. Wester,Mike Danielson,Kyle S. Michel,Aaron K. Neumann +5 more
TL;DR: Results indicate that Dectin-1A senses the solution conformation of β-glucans through their varying ability to drive receptor dimer/oligomer formation and activation of membrane proximal signaling events.
Posted ContentDOI
Targeted antifungal liposomes
Suresh Ambati,Aileen R. Ferarro,S. Earl Kang,Xiaorong Lin,Michelle Momany,Zachary A. Lewis,Richard B. Meagher +6 more
TL;DR: A novel protocol coated anti-fungal drug loaded liposomes to fungal cell walls with a beta-glucan binding protein and thereby increased drug effectiveness by an order of magnitude.
References
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