Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor.
Jun Lan,Jiwan Ge,Jinfang Yu,Sisi Shan,Huan Zhou,Shilong Fan,Qi Zhang,Xuanling Shi,Qisheng Wang,Linqi Zhang,Xinquan Wang +10 more
TLDR
High-resolution crystal structures of the receptor-binding domain of the spike protein of SARS-CoV-2 and SARS -CoV in complex with ACE2 provide insights into the binding mode of these coronaviruses and highlight essential ACE2-interacting residues.Abstract:
A new and highly pathogenic coronavirus (severe acute respiratory syndrome coronavirus-2, SARS-CoV-2) caused an outbreak in Wuhan city, Hubei province, China, starting from December 2019 that quickly spread nationwide and to other countries around the world1–3. Here, to better understand the initial step of infection at an atomic level, we determined the crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2. The overall ACE2-binding mode of the SARS-CoV-2 RBD is nearly identical to that of the SARS-CoV RBD, which also uses ACE2 as the cell receptor4. Structural analysis identified residues in the SARS-CoV-2 RBD that are essential for ACE2 binding, the majority of which either are highly conserved or share similar side chain properties with those in the SARS-CoV RBD. Such similarity in structure and sequence strongly indicate convergent evolution between the SARS-CoV-2 and SARS-CoV RBDs for improved binding to ACE2, although SARS-CoV-2 does not cluster within SARS and SARS-related coronaviruses1–3,5. The epitopes of two SARS-CoV antibodies that target the RBD are also analysed for binding to the SARS-CoV-2 RBD, providing insights into the future identification of cross-reactive antibodies. High-resolution crystal structures of the receptor-binding domain of the spike protein of SARS-CoV-2 and SARS-CoV in complex with ACE2 provide insights into the binding mode of these coronaviruses and highlight essential ACE2-interacting residues.read more
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Renal dysfunction and prognosis of COVID-19 patients: a hospital-based retrospective cohort study.
TL;DR: Wang et al. as mentioned in this paper found that male gender, older age and hypertension were three importantly independent risk factors for renal dysfunction in COVID-19 patients, and at least one renal function marker of 3.33% patients remained abnormal in 2 weeks after discharge.
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Identifying the Zoonotic Origin of SARS-CoV-2 by Modeling the Binding Affinity between the Spike Receptor-Binding Domain and Host ACE2.
TL;DR: This work proposes a new structure-based approach to general zoonotic origin and susceptibility analyses that are critical for human infectious disease control and wildlife protection and suggests a limited range of potential intermediate SARS-CoV-2 hosts for further experimental investigation.
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The race to treat COVID-19: Potential therapeutic agents for the prevention and treatment of SARS-CoV-2.
Shagufta,Irshad Ahmad +1 more
TL;DR: In this paper, a review has been presented which discusses the structure and the activity of numerous molecules exhibiting promising SARS-CoV-2 and other CoVs inhibition activities, and the review offers an overview of the structure, a plausible mechanism of action, and crucial structural features substantial to inhibit the primary virus-based and host-based targets involved in SARS CoV2 treatment.
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In Silico Investigation of Spice Molecules as Potent Inhibitor of SARS-CoV-2
TL;DR: Though all the molecules bind actively with the SARS-CoV-2 RBD Spro and Mpro, but Piperine has the highest binding affinity among the 30 screened molecules, and the comparative study between Piperine and currently used drugs show that Piperine is more effective.
Journal ArticleDOI
Lipid-based vaccine nanoparticles for induction of humoral immune responses against HIV-1 and SARS-CoV-2.
Kyung Soo Park,Joseph Bazzill,Sejin Son,Jutaek Nam,Seung Won Shin,Lukasz J. Ochyl,Jeanne A. Stuckey,Jennifer L. Meagher,Louise Chang,Jun Song,David C. Montefiori,Celia C. LaBranche,Janet L. Smith,Jie Xu,James J. Moon +14 more
TL;DR: In this paper, a lipid-based nanoparticle vaccine platform (NVP) was proposed for vaccination against corona virus disease 2019 (COVID-19) highlighting the urgent need for vaccine systems that can generate potent and protective immune responses.
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