Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor.
Jun Lan,Jiwan Ge,Jinfang Yu,Sisi Shan,Huan Zhou,Shilong Fan,Qi Zhang,Xuanling Shi,Qisheng Wang,Linqi Zhang,Xinquan Wang +10 more
TLDR
High-resolution crystal structures of the receptor-binding domain of the spike protein of SARS-CoV-2 and SARS -CoV in complex with ACE2 provide insights into the binding mode of these coronaviruses and highlight essential ACE2-interacting residues.Abstract:
A new and highly pathogenic coronavirus (severe acute respiratory syndrome coronavirus-2, SARS-CoV-2) caused an outbreak in Wuhan city, Hubei province, China, starting from December 2019 that quickly spread nationwide and to other countries around the world1–3. Here, to better understand the initial step of infection at an atomic level, we determined the crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2. The overall ACE2-binding mode of the SARS-CoV-2 RBD is nearly identical to that of the SARS-CoV RBD, which also uses ACE2 as the cell receptor4. Structural analysis identified residues in the SARS-CoV-2 RBD that are essential for ACE2 binding, the majority of which either are highly conserved or share similar side chain properties with those in the SARS-CoV RBD. Such similarity in structure and sequence strongly indicate convergent evolution between the SARS-CoV-2 and SARS-CoV RBDs for improved binding to ACE2, although SARS-CoV-2 does not cluster within SARS and SARS-related coronaviruses1–3,5. The epitopes of two SARS-CoV antibodies that target the RBD are also analysed for binding to the SARS-CoV-2 RBD, providing insights into the future identification of cross-reactive antibodies. High-resolution crystal structures of the receptor-binding domain of the spike protein of SARS-CoV-2 and SARS-CoV in complex with ACE2 provide insights into the binding mode of these coronaviruses and highlight essential ACE2-interacting residues.read more
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Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies.
Xing Zhu,Dhiraj Mannar,Shanti Swaroop Srivastava,Alison M. Berezuk,Jean-Philippe Demers,James W. Saville,Karoline Leopold,Wei Li,Dimiter S. Dimitrov,Katharine Tuttle,Steven Zhou,Sagar Chittori,Sriram Subramaniam +12 more
TL;DR: In this article, a 2.9-A resolution structure of the complex between the ACE2 receptor and N501Y spike protein ectodomains was presented, showing that Y501 inserted into a cavity at the binding interface near Y41 of ACE2.
Journal ArticleDOI
Comorbidities in SARS-CoV-2 Patients: a Systematic Review and Meta-Analysis.
Wern Hann Ng,Thomas Tipih,Nigel A. Makoah,Jan-G Vermeulen,Dominique Goedhals,Dominique Goedhals,Joseph Sempa,Joseph Sempa,Felicity J. Burt,Felicity J. Burt,Adam Taylor,Suresh Mahalingam +11 more
TL;DR: In this article, a systematic review was conducted using data from MEDLINE, Scopus, Web of Science, and EMBASE databases published from 1 December 2019 to 15 September 2020 to evaluate the correlation between comorbidities and their role in the exacerbation of disease in COVID-19 patients leading to fatal outcomes.
Journal ArticleDOI
COVID-19-associated gastrointestinal and liver injury: clinical features and potential mechanisms.
Peijie Zhong,Jing Xu,Dong Yang,Yue Shen,Lu Wang,Yun Feng,Chunling Du,Yuanlin Song,Chaomin Wu,Xianglin Hu,Yangbai Sun +10 more
TL;DR: COVID-19-related liver injury may be due to drug-induced liver injury, systemic inflammatory reaction, and hypoxia–ischemia reperfusion injury, and the direct toxic attack of SARS-CoV-2 on the liver is still questionable.
Journal ArticleDOI
The SARS-CoV-2 Exerts a Distinctive Strategy for Interacting with the ACE2 Human Receptor.
TL;DR: This work compares the interaction between the human ACE2 receptor and the SARS-CoV-2 spike protein with that of other pathogenic coronaviruses using molecular dynamics simulations and postulates that the versatility of cell receptor binding strategies has immediate implications for therapeutic strategies.
Journal ArticleDOI
A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2).
Jong Hwan Lee,Minsuk Choi,Yu-Jin Jung,Sung Kyun Lee,Chang-Seop Lee,Jung Kim,Jongwoo Kim,Nam Hoon Kim,Bum Tae Kim,Hong Gi Kim +9 more
TL;DR: This is the first study to detect SARS-CoV-2 S1 antigen using an LFIA with matched pair consisting of ACE2 and antibody, which can form matched pairs with commercially available antibodies.
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TL;DR: Identification and characterization of a new coronavirus (2019-nCoV), which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China, and it is shown that this virus belongs to the species of SARSr-CoV, indicates that the virus is related to a bat coronav virus.
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