Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor.
Jun Lan,Jiwan Ge,Jinfang Yu,Sisi Shan,Huan Zhou,Shilong Fan,Qi Zhang,Xuanling Shi,Qisheng Wang,Linqi Zhang,Xinquan Wang +10 more
TLDR
High-resolution crystal structures of the receptor-binding domain of the spike protein of SARS-CoV-2 and SARS -CoV in complex with ACE2 provide insights into the binding mode of these coronaviruses and highlight essential ACE2-interacting residues.Abstract:
A new and highly pathogenic coronavirus (severe acute respiratory syndrome coronavirus-2, SARS-CoV-2) caused an outbreak in Wuhan city, Hubei province, China, starting from December 2019 that quickly spread nationwide and to other countries around the world1–3. Here, to better understand the initial step of infection at an atomic level, we determined the crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 bound to the cell receptor ACE2. The overall ACE2-binding mode of the SARS-CoV-2 RBD is nearly identical to that of the SARS-CoV RBD, which also uses ACE2 as the cell receptor4. Structural analysis identified residues in the SARS-CoV-2 RBD that are essential for ACE2 binding, the majority of which either are highly conserved or share similar side chain properties with those in the SARS-CoV RBD. Such similarity in structure and sequence strongly indicate convergent evolution between the SARS-CoV-2 and SARS-CoV RBDs for improved binding to ACE2, although SARS-CoV-2 does not cluster within SARS and SARS-related coronaviruses1–3,5. The epitopes of two SARS-CoV antibodies that target the RBD are also analysed for binding to the SARS-CoV-2 RBD, providing insights into the future identification of cross-reactive antibodies. High-resolution crystal structures of the receptor-binding domain of the spike protein of SARS-CoV-2 and SARS-CoV in complex with ACE2 provide insights into the binding mode of these coronaviruses and highlight essential ACE2-interacting residues.read more
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Rapid generation of potent antibodies by autonomous hypermutation in yeast.
Alon Wellner,Conor McMahon,Conor McMahon,Morgan S.A. Gilman,Jonathan R. Clements,Sarah Clark,Kianna M. Nguyen,Ming H. Ho,Vincent J. Hu,Jung-Eun Shin,Jared Feldman,Blake M. Hauser,Timothy M. Caradonna,Laura M. Wingler,Laura M. Wingler,Aaron G. Schmidt,Aaron G. Schmidt,Debora S. Marks,Debora S. Marks,Jonathan Abraham,Jonathan Abraham,Jonathan Abraham,Andrew C. Kruse,Chang C. Liu +23 more
TL;DR: In this paper, an autonomous hypermutation yeast surface display (AHEAD) was proposed to generate potent nanobodies against the SARS-CoV-2 S glycoprotein, a G-protein-coupled receptor and other targets.
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Coronavirus (SARS-CoV-2) Pandemic: Future Challenges for Dental Practitioners.
Ioana Roxana Bordea,Edit Xhajanka,Sebastian Candrea,Simion Bran,Florin Onișor,Alessio Danilo Inchingolo,G Malcangi,Van Hung Pham,Angelo M Inchingolo,Antonio Scarano,Felice Lorusso,Ciro Gargiulo Isacco,Sergey K. Aityan,Andrea Ballini,Gianna Dipalma,Francesco Inchingolo +15 more
TL;DR: The way in which SARS-CoV-2 spreads, how to diagnose a novel corona virus infection, what the possible treatments are, and which protective personal equipment the authors can use to stop its spreading are made.
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Novel ACE2-IgG1 fusions with improved activity against SARS-CoV2
Naoki Iwanaga,Laura Cooper,Lijun Rong,Brandon J. Beddingfield,Jackelyn Crabtree,Ralph A. Tripp,Jay K. Kolls +6 more
TL;DR: In this paper, an ACE2-human IgG1 fusion protein with mutations in the catalytic domain of ACE2 was constructed, which contained a LALA mutation that abrogates Fcrγ binding, but retains FcRN binding to prolong the half-life and achieve therapeutic concentrations in the lung lavage.
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Emergence and outcomes of the SARS-CoV-2 'Marseille-4' variant.
Pierre-Edouard Fournier,Philippe Colson,Anthony Levasseur,Christian Devaux,Philippe Gautret,Marielle Bedotto,Jeremy Delerce,Ludivine Brechard,Lucile Pinault,Jean-Christophe Lagier,Florence Fenollar,Didier Raoult +11 more
TL;DR: The Marseille-4 variant caused an epidemic that started in August and is still ongoing as discussed by the authors, and it caused 12-100% of SARS-CoV-2 infections in Marseille from September 2020.
Journal ArticleDOI
SARS-CoV-2 spike protein: pathogenesis, vaccines, and potential therapies.
Ahmed M. Almehdi,Ghalia Khoder,Aminah S. Alchakee,Azizeh T. Alsayyid,Nadin H. Sarg,Sameh S. M. Soliman +5 more
TL;DR: A literature review was conducted to summarize, analyze, and interpret the available scientific data related to SARS-CoV-2 S protein in terms of characteristics, vaccines development and potential therapies as mentioned in this paper.
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