Syrian hamsters as a small animal model for SARS-CoV-2 infection and countermeasure development.
Masaki Imai,Kiyoko Iwatsuki-Horimoto,Masato Hatta,Samantha Loeber,Peter Halfmann,Noriko Nakajima,Tokiko Watanabe,Michiko Ujie,Kenta Takahashi,Mutsumi Ito,Shinya Yamada,Shufang Fan,Shiho Chiba,Makoto Kuroda,Lizheng Guan,Kosuke Takada,Tammy Armbrust,Aaron Balogh,Yuri Furusawa,Moe Okuda,Hiroshi Ueki,Atsuhiro Yasuhara,Yuko Sakai-Tagawa,Tiago J. S. Lopes,Tiago J. S. Lopes,Maki Kiso,Seiya Yamayoshi,Noriko Kinoshita,Norio Ohmagari,Shin-ichiro Hattori,Makoto Takeda,Hiroaki Mitsuya,Florian Krammer,Tadaki Suzuki,Yoshihiro Kawaoka,Yoshihiro Kawaoka +35 more
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TLDR
It is found that SARS-CoV-2 isolates replicate efficiently in the lungs of Syrian hamsters and cause severe pathological lesions in the lung of these animals similar to commonly reported imaging features of COVID-19 patients with pneumonia.Abstract:
At the end of 2019, a novel coronavirus (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2) was detected in Wuhan, China, that spread rapidly around the world, with severe consequences for human health and the global economy Here, we assessed the replicative ability and pathogenesis of SARS-CoV-2 isolates in Syrian hamsters SARS-CoV-2 isolates replicated efficiently in the lungs of hamsters, causing severe pathological lung lesions following intranasal infection In addition, microcomputed tomographic imaging revealed severe lung injury that shared characteristics with SARS-CoV-2-infected human lung, including severe, bilateral, peripherally distributed, multilobular ground glass opacity, and regions of lung consolidation SARS-CoV-2-infected hamsters mounted neutralizing antibody responses and were protected against subsequent rechallenge with SARS-CoV-2 Moreover, passive transfer of convalescent serum to naive hamsters efficiently suppressed the replication of the virus in the lungs even when the serum was administrated 2 d postinfection of the serum-treated hamsters Collectively, these findings demonstrate that this Syrian hamster model will be useful for understanding SARS-CoV-2 pathogenesis and testing vaccines and antiviral drugsread more
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Hamsters Expressing Human Angiotensin-Converting Enzyme 2 Develop Severe Disease following Exposure to SARS-CoV-2
Joseph W. Golden,Rongxiu Li,Curtis R. Cline,Xiankun Zeng,Eric M. Mucker,Amadeo J. Fuentes-Lao,Kristin Spik,Janice A Williams,Nancy A. Twenhafel,N. Davis,Joshua L. Moore,Stephen Stevens,E. Blue,Aura R. Garrison,Deanna D. Larson,Rebekah Stewart,Madelyn Kunzler,Yanan Liu,Zhongde Wang,Jay W. Hooper +19 more
TL;DR: This work studied SARS-CoV-2 infection in hamsters engineered to express the human angiotensin-converting enzyme 2 viral receptor under the control of the K18 promoter, which produces a severe and lethal infection in transgenic hamsters that mirrors the most severe aspects of COVID-19 in humans, including respiratory and neurological injury.
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Parsing the role of NSP1 in SARS-CoV-2 infection
Tal Fisher,Avi Gluck,Krishna Narayanan,Makoto Kuroda,Aharon Nachshon,Jason Hsu,Peter Halfmann,Yfat Yahalom-Ronen,Yaara Finkel,Michal Schwartz,Shay Weiss,Chien Te K. Tseng,Tomer Israely,Nir Paran,Yoshihiro Kawaoka,Shinji Makino,Noam Stern-Ginossar +16 more
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Attenuated influenza virions expressing the SARS-CoV-2 receptor-binding domain induce neutralizing antibodies in mice
Andrea N. Loes,Andrea N. Loes,Lauren E. Gentles,Lauren E. Gentles,Allison J. Greaney,Allison J. Greaney,Katharine H.D. Crawford,Katharine H.D. Crawford,Jesse D. Bloom +8 more
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References
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TL;DR: It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.
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Presenting Characteristics, Comorbidities, and Outcomes Among 5700 Patients Hospitalized With COVID-19 in the New York City Area.
Safiya Richardson,Safiya Richardson,Jamie S. Hirsch,Jamie S. Hirsch,Mangala Narasimhan,James M. Crawford,Thomas McGinn,Thomas McGinn,Karina W. Davidson,Karina W. Davidson,Douglas P. Barnaby,Douglas P. Barnaby,Lance B Becker,John Chelico,John Chelico,Stuart L. Cohen,Stuart L. Cohen,Jennifer Cookingham,Kevin Coppa,Michael A Diefenbach,Andrew J. Dominello,Joan Duer-Hefele,Louise Falzon,Jordan Gitlin,Negin Hajizadeh,Negin Hajizadeh,Tiffany G. Harvin,David Hirschwerk,Eun Ji Kim,Eun Ji Kim,Zachary Kozel,Lyndonna Marrast,Lyndonna Marrast,Jazmin N. Mogavero,Gabrielle A. Osorio,Michael Qiu,Theodoros P. Zanos +36 more
TL;DR: This case series provides characteristics and early outcomes of sequentially hospitalized patients with confirmed COVID-19 in the New York City area and assesses outcomes during hospitalization, such as invasive mechanical ventilation, kidney replacement therapy, and death.
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