Journal ArticleDOI
The application of a G-quadruplex based assay with an iridium(iii) complex to arsenic ion detection and its utilization in a microfluidic chip
Sheng Lin,Wanhe Wang,Chong Hu,Guan-Jun Yang,Chung-Nga Ko,Kangning Ren,Chung-Hang Leung,Dik-Lung Ma +7 more
TLDR
The iridium(iii) complex 1 was synthesized and employed in constructing an assay which is based on a G-quadruplex for detecting arsenic ions in aqueous solution and showed high selectivity towards arsenic ions over other metal ions.Abstract:
In this work, the iridium(iii) complex 1 was synthesized and employed in constructing an assay which is based on a G-quadruplex for detecting arsenic ions in aqueous solution. The assay achieved a detection limit of 7.6 nM (ca. 0.57 μg L-1) and showed high selectivity towards arsenic ions over other metal ions. Additionally, the assay could function in natural water and a simple microfluidic chip was used to investigate the potential of this platform for real-time detection.read more
Citations
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Journal ArticleDOI
Nanomaterial-based aptamer sensors for arsenic detection
TL;DR: The recent development and applications of aptamer sensors (aptasensors) based-on nanomaterial for arsenic detection are reviewed, in particular with emphasis on the works using optical and electrochemical technologies.
Journal ArticleDOI
Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.
Christine F. Markwalter,Andrew G. Kantor,Carson Paige Moore,Kelly A. Richardson,David W. Wright +4 more
TL;DR: This review of works from the past 20 years in which inorganic chemistry and nanotechnology were implemented in each of the core components that make up a diagnostic test highlights the detection of nucleic acids at the point of care as an emerging application of inorganic Chemistry.
Journal ArticleDOI
An electrochemiluminescence cloth-based biosensor with smartphone-based imaging for detection of lactate in saliva.
TL;DR: The presented ECL platform has the potential to be applied to non-invasively detect a variety of analytes of medical interest, and the ECL biosensor is successfully applied for the determination of lactate in human saliva.
Journal ArticleDOI
Green synthesis of surface-passivated carbon dots from the prickly pear cactus as a fluorescent probe for the dual detection of arsenic( iii ) and hypochlorite ions from drinking water
TL;DR: In this article, a simple sensor based on Glutathione (GSH) was developed for the green synthesis of surface-passivated carbon dots from edible prickly pear cactus fruit as the carbon source by a one-pot hydrothermal route.
Journal ArticleDOI
Thiazole Orange-Modified Carbon Dots for Ratiometric Fluorescence Detection of G-Quadruplex and Double-Stranded DNA.
TL;DR: The ratiometric fluorescence nanoprobe constructed in this work exhibited high sensitivity for ssab (a dsDNA) and c-myc (a parallel G4) with a low detection limit of 0.90 and 3.31 nM, respectively and showed good selectivity to parallel G-quadruplex (G4) and double-stranded (ds) DNA over antiparallel G4 and single- Stranded DNA.
References
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Journal ArticleDOI
A simple and sensitive resonance Rayleigh scattering method for determination of As(III) using aptamer-modified nanogold as a probe.
TL;DR: A simple and selective aptamer (ssDNA)-modified nanogold probe (AussDNA) was prepared for the determination of trace As(III) in HEPES buffer solution (pH 8.2) containing 0.05 mol/L NaCl and this RRS method was applied to detect As( III) in water samples, with simplicity, sensitivity and selectivity.
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Exploded view of higher order G-quadruplex structures through click-chemistry assisted single-molecule mechanical unfolding.
TL;DR: This work used laser tweezers to mechanically dissect high-order DNA structures into two constituting G-quadruplexes in the promoter of the human telomerase reverse transcriptase (hTERT) gene, offering unprecedented perspective to decipher dynamic transitions in complex biomacromolecules.
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Oxidation State-Differentiated Measurement of Aqueous Inorganic Arsenic by Continuous Flow Electrochemical Arsine Generation Coupled to Gas-Phase Chemiluminescence Detection
TL;DR: The basic electrochemical arsine generation technique and current-differentiated oxidation state speciation should be applicable as the front end to many other arsenic measurements techniques, including atomic spectrometry.
Journal ArticleDOI
Identification of human telomerase assembly inhibitors enabled by a novel method to produce hTERT
Guillaume Kellermann,Markus Kaiser,Florent Dingli,Olivier Lahuna,Delphine Naud-Martin,Florence Mahuteau-Betzer,Damarys Loew,Evelyne Ségal-Bendirdjian,Marie-Paule Teulade-Fichou,Sophie Bombard,Sophie Bombard +10 more
TL;DR: A breakthrough method to produce unprecedented amounts of recombinant hTERT and to reconstitute human telomerase with purified components and to identify regulators of the assembly of this ribonucleoprotein complex is presented.
Journal ArticleDOI
An electrochemical-sensor system for real-time flow measurements in porous materials.
TL;DR: The proposed electrochemical-sensing method offers a valuable alternative to existing optical-based monitoring techniques for flow measurement in paper-based microfluidic systems and allows the in situ monitoring of the different hydrodynamic conditions of a specific paper geometry or composition.