The self-stirred genome: large-scale chromatin dynamics, its biophysical origins and implications
TLDR
Activity of nuclear enzymes was found to drive the coherent chromatin dynamics, however, its biological nature and physical mechanism remain to be revealed.About:
This article is published in Current Opinion in Genetics & Development.The article was published on 2020-04-01 and is currently open access. It has received 23 citations till now. The article focuses on the topics: Chromatin.read more
Citations
More filters
Long-Range Directional Movement of an Interphase Chromosome Site Dependent on Actin and Nuclear Myosin
TL;DR: The results suggest an active mechanism for fast and directed long-range interphase chromosome movements dependent directly or indirectly on actin/myosin.
Journal Article
Real-time dynamics of RNA Polymerase II clustering in live human cells
TL;DR: Rickman et al. as discussed by the authors developed a quantitative single-cell approach to characterize protein spatiotemporal organization, with single-molecule sensitivity in live eukaryotic cells.
Journal ArticleDOI
The rich inner life of the cell nucleus: dynamic organization, active flows, and emergent rheology
TL;DR: It is proposed that the nucleus presents a highly heterogeneous and dynamic environment with diverse functionality and its biophysical complexity can be organized around three inter-related and interactive facets: heterogeneity, activity, and rheology.
Journal ArticleDOI
Multiscale modeling of genome organization with maximum entropy optimization.
TL;DR: In this article, the authors review the progress that has been made in modeling the human genome and conclude with an outlook on the opportunities and challenges in studying chromosome dynamics, and provide insight into the principles of whole-genome organization and enable de novo predictions of chromosome structures from epigenetic modifications.
Journal ArticleDOI
Interphase Chromatin Undergoes a Local Sol-Gel Transition upon Cell Differentiation
TL;DR: In this article, the authors measured the chromatin distribution and dynamics in embryonic stem cells in vivo before and after differentiation and found that undifferentiated chromatin is less compact, more homogeneous, and more dynamic than differentiated chromatin.
References
More filters
Journal ArticleDOI
Comprehensive mapping of long-range interactions reveals folding principles of the human genome.
Erez Lieberman Aiden,Nynke L. van Berkum,Louise Williams,Maxim Imakaev,Tobias Ragoczy,Tobias Ragoczy,Agnes Telling,Agnes Telling,Ido Amit,Bryan R. Lajoie,Peter J. Sabo,Michael O. Dorschner,Richard Sandstrom,Bradley E. Bernstein,Bradley E. Bernstein,Michaël Bender,Mark Groudine,Mark Groudine,Andreas Gnirke,John A. Stamatoyannopoulos,Leonid A. Mirny,Eric S. Lander,Eric S. Lander,Job Dekker +23 more
TL;DR: Hi-C is described, a method that probes the three-dimensional architecture of whole genomes by coupling proximity-based ligation with massively parallel sequencing and demonstrates the power of Hi-C to map the dynamic conformations of entire genomes.
Journal ArticleDOI
Central Dogma of Molecular Biology
TL;DR: The central dogma of molecular biology deals with the detailed residue-by-residue transfer of sequential information and states that such information cannot be transferred from protein to either protein or nucleic acid.
Journal ArticleDOI
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System
Baohui Chen,Luke A. Gilbert,Beth A. Cimini,Joerg Schnitzbauer,Wei Zhang,Gene-Wei Li,Jason Park,Elizabeth H. Blackburn,Jonathan S. Weissman,Lei S. Qi,Bo Huang +10 more
TL;DR: Using an EGFP-tagged endonuclease-deficient Cas9 protein and a structurally optimized small guide (sg) RNA, robust imaging of repetitive elements in telomeres and coding genes in living cells is demonstrated by repurposing the bacterial CRISPR/Cas system.
Journal ArticleDOI
Coexisting Liquid Phases Underlie Nucleolar Subcompartments
Marina Feric,Nilesh Vaidya,Tyler S. Harmon,Diana M. Mitrea,Lian Zhu,Tiffany M. Richardson,Richard W. Kriwacki,Rohit V. Pappu,Clifford P. Brangwynne +8 more
TL;DR: It is shown that subcompartments within the nucleolus represent distinct, coexisting liquid phases that may facilitate sequential RNA processing reactions in a variety of RNP bodies, and suggested that phase separation can give rise to multilayered liquids.
Journal ArticleDOI
Phase separation drives heterochromatin domain formation
Amy R. Strom,Alexander Emelyanov,Mustafa Mir,Dmitry V. Fyodorov,Xavier Darzacq,Gary H. Karpen,Gary H. Karpen +6 more
TL;DR: It is proposed that emergent biophysical properties associated with phase-separated systems are critical to understanding the unusual behaviours of heterochromatin, and how chromatin domains in general regulate essential nuclear functions.
Related Papers (5)
Making copies of chromatin: the challenge of nucleosomal organization and epigenetic information
Monitoring global chromatin dynamics in response to DNA damage.
Haitham A. Shaban,Andrew Seeber +1 more