Journal ArticleDOI
Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics
Majid Ebrahimi Warkiani,Majid Ebrahimi Warkiani,Bee Luan Khoo,Bee Luan Khoo,Lidan Wu,Andy Tay,Andy Tay,Ali Asgar S. Bhagat,Jongyoon Han,Jongyoon Han,Chwee Teck Lim,Chwee Teck Lim +11 more
Reads0
Chats0
TLDR
This protocol describes detailed procedures for the production and use of a label-free spiral microfluidic device to allow size-based isolation of viable CTCs using hydrodynamic forces that are present in curvilinear microchannels.Abstract:
Circulating tumor cells (CTCs) are rare cancer cells that are shed from primary or metastatic tumors into the peripheral blood circulation. Phenotypic and genetic characterization of these rare cells can provide important information to guide cancer staging and treatment, and thus further research into their characteristics and properties is an area of considerable interest. In this protocol, we describe detailed procedures for the production and use of a label-free spiral microfluidic device to allow size-based isolation of viable CTCs using hydrodynamic forces that are present in curvilinear microchannels. This spiral system enables us to achieve ≥ 85% recovery of spiked cells across multiple cancer cell lines and 99.99% depletion of white blood cells in whole blood. The described spiral microfluidic devices can be produced at an extremely low cost using standard microfabrication and soft lithography techniques (2-3 d), and they can be operated using two syringe pumps for lysed blood samples (7.5 ml in 12.5 min for a three-layered multiplexed chip). The fast processing time and the ability to collect CTCs from a large patient blood volume allows this technique to be used experimentally in a broad range of potential genomic and transcriptomic applications.read more
Citations
More filters
Journal ArticleDOI
Homophilic CD44 Interactions Mediate Tumor Cell Aggregation and Polyclonal Metastasis in Patient-Derived Breast Cancer Models
Xia Liu,Rokana Taftaf,Madoka Kawaguchi,Ya Fang Chang,Wenjing Chen,David Entenberg,Youbin Zhang,Lorenzo Gerratana,Lorenzo Gerratana,Simo Huang,Dhwani Patel,Elizabeth Tsui,Valery Adorno-Cruz,Valery Adorno-Cruz,Steven M. Chirieleison,Yue Cao,Allison S. Harney,Shivani A. Patel,Antonia Patsialou,Yang Shen,Stefanie Avril,Hannah Gilmore,Justin D. Lathia,Justin D. Lathia,Derek W. Abbott,Massimo Cristofanilli,John S. Condeelis,Huiping Liu +27 more
TL;DR: It is found that clustered tumor cells in migration and circulation resulted from aggregation of individual tumor cells rather than collective migration and cohesive shedding, which will lead to innovative biomarker applications to predict prognosis, facilitate development of new targeting strategies to block polyclonal metastasis, and improve clinical outcomes.
Journal ArticleDOI
Low-cost thermophoretic profiling of extracellular-vesicle surface proteins for the early detection and classification of cancers.
Chao Liu,Chao Liu,Junxiang Zhao,Junxiang Zhao,Fei Tian,Lili Cai,Wei Zhang,Wei Zhang,Qiang Feng,Jianqiao Chang,Fangning Wan,Yunjie Yang,Bo Dai,Yulong Cong,Baoquan Ding,Baoquan Ding,Jiashu Sun,Jiashu Sun,Weihong Tan +18 more
TL;DR: An assay that thermophoretically profiles surface proteins from serum extracellular vesicles labelled with a panel of fluorescent aptamers detects and classifies patients according to cancer type and cancer stage, and if validated in larger cohorts may facilitate cancer screening, classification and monitoring.
Journal ArticleDOI
Dean Flow Dynamics in Low-Aspect Ratio Spiral Microchannels.
TL;DR: This work is the first to experimentally and numerically investigate Dean flows in microchannels for Re > 100, and show presence of secondary Dean vortices beyond a critical Dean number, and offers new insights into secondary flow instabilities for low-aspect ratio, spiral microch channels.
Journal ArticleDOI
Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC‐MS
Ying Zhu,Geremy Clair,William B. Chrisler,Yufeng Shen,Rui Zhao,Anil K. Shukla,Ronald J. Moore,Ravi S. Misra,Gloria S. Pryhuber,Richard D. Smith,Charles Ansong,Ryan T. Kelly +11 more
TL;DR: It is demonstrated that the single-cell proteomics platform can be used to differentiate cell types from enzyme-dissociated human lung primary cells and identify specific protein markers for epithelial and mesenchymal cells.
Journal ArticleDOI
Cancer diagnosis: from tumor to liquid biopsy and beyond
TL;DR: This review discusses the research progress in this field with respect to each of the liquid biopsy markers ranging from CTCs, EVs to ctDNA, and highlights key CTC technologies that have been commercialized and extensively employed for patient sample analysis.
References
More filters
Journal ArticleDOI
Global cancer statistics
TL;DR: A substantial proportion of the worldwide burden of cancer could be prevented through the application of existing cancer control knowledge and by implementing programs for tobacco control, vaccination, and early detection and treatment, as well as public health campaigns promoting physical activity and a healthier dietary intake.
Journal ArticleDOI
Prospective identification of tumorigenic breast cancer cells
Muhammad Al-Hajj,Max S. Wicha,Adalberto Benito-Hernandez,Sean J. Morrison,Sean J. Morrison,Michael F. Clarke +5 more
TL;DR: The ability to prospectively identify tumorigenic cancer cells will facilitate the elucidation of pathways that regulate their growth and survival and strategies designed to target this population may lead to more effective therapies.
Journal ArticleDOI
The origins and the future of microfluidics
TL;DR: The manipulation of fluids in channels with dimensions of tens of micrometres — microfluidics — has emerged as a distinct new field that has the potential to influence subject areas from chemical synthesis and biological analysis to optics and information technology.
Journal ArticleDOI
Cancer Metastasis: Building a Framework
Gaorav P. Gupta,Joan Massagué +1 more
TL;DR: Understanding of the origins and nature of cancer metastasis and the selection of traits that are advantageous to cancer cells is promoted.
Journal ArticleDOI
Isolation of rare circulating tumour cells in cancer patients by microchip technology.
Sunitha Nagrath,Lecia V. Sequist,Shyamala Maheswaran,Daphne W. Bell,Daphne W. Bell,Daniel Irimia,Lindsey Ulkus,Matthew R. Smith,Eunice L. Kwak,Subba R. Digumarthy,Alona Muzikansky,Paula D. Ryan,Ulysses J. Balis,Ulysses J. Balis,Ronald G. Tompkins,Daniel A. Haber,Mehmet Toner +16 more
TL;DR: The CTC-chip successfully identified CTCs in the peripheral blood of patients with metastatic lung, prostate, pancreatic, breast and colon cancer in 115 of 116 samples, with a range of 5–1,281CTCs per ml and approximately 50% purity.
Related Papers (5)
Isolation of rare circulating tumour cells in cancer patients by microchip technology.
Isolation of circulating tumor cells using a microvortex-generating herringbone-chip
Shannon L. Stott,Chia-Hsien Hsu,Chia-Hsien Hsu,Chia-Hsien Hsu,Dina Tsukrov,Min Yu,David T. Miyamoto,Belinda A. Waltman,S. Michael Rothenberg,Ajay Shah,Malgorzata E. Smas,George K. Korir,Frederick P. Floyd,Anna J. Gilman,Jenna B. Lord,Daniel Winokur,Simeon Springer,Daniel Irimia,Daniel Irimia,Sunitha Nagrath,Sunitha Nagrath,Lecia V. Sequist,Lecia V. Sequist,Richard T. Lee,Richard T. Lee,Kurt J. Isselbacher,Shyamala Maheswaran,Daniel A. Haber,Daniel A. Haber,Mehmet Toner,Mehmet Toner +30 more
Inertial Focusing for Tumor Antigen–Dependent and –Independent Sorting of Rare Circulating Tumor Cells
Emre Ozkumur,Ajay Shah,Jordan C. Ciciliano,Benjamin L. Emmink,David T. Miyamoto,Elena F. Brachtel,Min Yu,Min Yu,Pin-i Chen,Bailey Morgan,Julie Trautwein,Anya Kimura,Sudarshana Sengupta,Shannon L. Stott,Nezihi Murat Karabacak,Tom Barber,John R. Walsh,Kyle C. Smith,Philipp S. Spuhler,James P. Sullivan,Richard T. Lee,David T. Ting,Xi Luo,Xi Luo,Alice T. Shaw,Aditya Bardia,Lecia V. Sequist,David N. Louis,Shyamala Maheswaran,Ravi Kapur,Daniel A. Haber,Daniel A. Haber,Mehmet Toner +32 more