National Dairy Research Institute

About: National Dairy Research Institute is a facility organization based out in Karnāl, Himachal Pradesh, India. It is known for research contribution in the topics: Population & Sperm. The organization has 3228 authors who have published 3524 publications receiving 51151 citations. The organization is also known as: Imperial Institute of Animal Husbandry and Dairying & Imperial Dairy Institute.

Anticarcinogenic Effect of Probiotic Dahi and Piroxicam on DMH-induced Colorectal Carcinogenesis in Wistar Rats

Abstract: This study examined the therapeutic effects of probiotic Dahi containing Lactobacillus acidophilus LaVK2 and Lactobacillus plantarum Lp9, alone or as well as in combination with piroxicam (PXC) as an antioxidant agent in male Wistar rats administered 1, 2-dimethylhydrazine dihydrochloride (DMH). Colorectal carcinogenesis was induced by injecting DMH subcutaneously (40 mg/kg body weight) twice a week for 2 weeks. The rats were fed with buffalo milk or probiotic Dahi alone or in combination with PXC in addition to basal diet ad libitum and euthanized at 8th, 16th and 32nd week of the experiment and examined for biochemical and histopathological profile. A significant reduction in lipid peroxidation measured as thiobarbituric acid reactive substances (TBARS) in liver and colorectal tissue, and carcinogen activating enzyme measured as b -glucuronidase activity in faeces were observed in probiotic Dahi-DMH-PXC group compared with the DMH control group. Probiotic Dahi alone or in combination with PXC was showed a significant (P < 0.05) protective effect by lowering the levels of TBARS, faecal b -glucuronidase and by enhancing the activity GST in liver and colorectal tissues, correlated with decreased tumor incidence, tumor multiplicity and tumor volume in gastrointestinal (GI) tract in DMH induced rats. These observations suggest that probiotic Dahi alone or conjointly with PXC may have therapeutic potential in attenuation of carcinogenesis in GI tract. Normal 0 7.8 磅 0 2 false false false EN-US ZH-CN X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:普通表格; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.5pt; mso-bidi-font-size:11.0pt; font-family:"Century","serif"; mso-ascii-font-family:Century; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:宋体; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Century; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-font-kerning:1.0pt;}

Heat stress and antioxidant enzyme activity in bubaline (Bubalus bubalis) oocytes during in vitro maturation

Abstract: In vitro environments like heat stress usually increase the production of reactive oxygen species in bubaline oocytes which have been implicated as one of the major causes for reduced developmental competence. Oocytes during meiotic maturation are sensitive to oxidative stress, and heat stress accelerates cellular metabolism, resulting in the higher production of free radicals. Therefore, the aim of present work was to assess the impact of heat stress during meiotic maturation on bubaline cumulus-oocyte complexes (COC), denuded oocytes (DO), and cumulus cell mass in terms of their oxidative status. Accordingly, for control group, COC were matured at 38.5 °C for complete 24 h of meiotic maturation and heat stress of 40.5 and 41.5 °C was applied to COC during the first 12 h of maturation and then moved to 38.5 °C for rest of the 12 h. In another group, COC after maturation were denuded from the surrounding cumulus cells by manual pipetting. Results indicated that the production of reactive oxygen species (ROS), lipid peroxides, and nitric oxide (NO) was significantly (P < 0.05) higher in the oocytes subjected to heat stress (40.5 and 41.5 °C) during meiotic maturation compared to the oocytes matured under standard in vitro culture conditions (38.5 °C). Also, the antioxidant enzymatic activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase were significantly (P < 0.05) increased in all the treatment groups compared to the control group. Therefore, the present study clearly establishes that heat stress ensues oxidative stress in bubaline oocytes which triggers the induction of antioxidant enzymatic defense system for scavenging the ROS.

Cellulolytic bacteria in buffalo rumen

Abstract: The influence of three different feeds, wheat straw, sorghum and berseem, on total and cellulolytic bacterial counts in the buffalo rumen at different time intervals from 0 to 8 h after feeding was studied. Berseem feeding supported maximum growth of rumen bacteria in general and cellulolytic bacteria in particular. Wheat straw supported the poorest growth. The types of cellulolytic bacteria recovered from the rumen of adult buffaloes were Ruminococcus albus, R. flavefaciens, Bacteroides succinogenes, Butyrivibrio fibrisolvens, Clostridium lochheadii, Cl. longisporum and other Clostridium spp. Cellulolytic cocci were present in smaller numbers than rod forms in the rumen of wheat-straw-fed buffaloes, whereas the cocci outnumbered rod forms in sorghum-and berseem-fed buffaloes.