Showing papers by "University of Hohenheim published in 2006"

Sources of CO2 efflux from soil and review of partitioning methods

Abstract: Five main biogenic sources of CO2 efflux from soils have been distinguished and described according to their turnover rates and the mean residence time of carbon. They are root respiration, rhizomicrobial respiration, decomposition of plant residues, the priming effect induced by root exudation or by addition of plant residues, and basal respiration by microbial decomposition of soil organic matter (SOM). These sources can be grouped in several combinations to summarize CO2 efflux from the soil including: root-derived CO2, plant-derived CO2, SOM-derived CO2, rhizosphere respiration, heterotrophic microbial respiration (respiration by heterotrophs), and respiration by autotrophs. These distinctions are important because without separation of SOM-derived CO2 from plant-derived CO2, measurements of total soil respiration have very limited value for evaluation of the soil as a source or sink of atmospheric CO2 and for interpreting the sources of CO2 and the fate of carbon within soils and ecosystems. Additionally, the processes linked to the five sources of CO2 efflux from soil have various responses to environmental variables and consequently to global warming. This review describes the basic principles and assumptions of the following methods which allow SOMderived and root-derived CO2 efflux to be separated under laboratory and field conditions: root exclusion techniques, shading and clipping, tree girdling, regression, component integration, excised roots and in situ root respiration; continuous and pulse labeling, 13 C natural abundance and FACE, and radiocarbon dating and bomb- 14 C. A short sections cover the separation of the respiration of autotrophs and that of heterotrophs, i.e. the separation of actual root respiration from microbial respiration, as well as methods allowing the amount of CO2 evolved by decomposition of plant residues and by priming effects to be estimated. All these methods have been evaluated according to their inherent disturbance of the ecosystem and C fluxes, and their versatility under various conditions. The shortfalls of existing approaches and the need for further development and standardization of methods are highlighted. q 2005 Elsevier Ltd. All rights reserved.

The Arabidopsis Major Intrinsic Protein NIP5;1 Is Essential for Efficient Boron Uptake and Plant Development under Boron Limitation

Abstract: Boron (B) is essential in plants but often present at low concentrations in the environment. To investigate how plants survive under conditions of B limitation, we conducted a transcriptome analysis and identified NIP5;1, a member of the major intrinsic protein family, as a gene upregulated in B-deficient roots of Arabidopsis thaliana. Promoter-beta-glucuronidase fusions indicated that NIP5;1 is strongly upregulated in the root elongation zone and the root hair zone under B limitation, and green fluorescent protein-tagged NIP5;1 proteins localized to the plasma membrane. Expression in Xenopus laevis oocytes demonstrated that NIP5;1 facilitated the transport of boric acid in addition to water. Importantly, two T-DNA insertion lines of NIP5;1 displayed lower boric acid uptake into roots, lower biomass production, and increased sensitivity of root and shoot development to B deficiency. These results identify NIP5;1 as a major plasma membrane boric acid channel crucial for the B uptake required for plant growth and development under B limitation.

Consumer–resource body-size relationships in natural food webs

Abstract: It has been suggested that differences in body size between consumer and resource species may have important implications for interaction strengths, population dynamics, and eventually food web structure, function, and evolution. Still, the general distribution of consumer-'resource body-size ratios in real ecosystems, and whether they vary systematically among habitats or broad taxonomic groups, is poorly understood. Using a unique global database on consumer and resource body sizes, we show that the mean body-size ratios of aquatic herbivorous and detritivorous consumers are several orders of magnitude larger than those of carnivorous predators. Carnivorous predator-prey body-size ratios vary across different habitats and predator and prey types (invertebrates, ectotherm, and endotherm vertebrates). Predator-prey body-size ratios are on average significantly higher (1) in freshwater habitats than in marine or terrestrial habitats, (2) for vertebrate than for invertebrate predators, and (3) for invertebrate than for ectotherm vertebrate prey. If recent studies that relate body-size ratios to interaction strengths are general, our results suggest that mean consumer-resource interaction strengths may vary systematically across different habitat categories and consumer types.

Abundance of narG, nirS, nirK, and nosZ genes of denitrifying bacteria during primary successions of a glacier foreland

Abstract: Quantitative PCR of denitrification genes encoding the nitrate, nitrite, and nitrous oxide reductases was used to study denitrifiers across a glacier foreland. Environmental samples collected at different distances from a receding glacier contained amounts of 16S rRNA target molecules ranging from 4.9 × 10 5 to 8.9 × 10 5 copies per nanogram of DNA but smaller amounts of narG , nirK , and nosZ target molecules. Thus, numbers of narG , nirK , nirS , and nosZ copies per nanogram of DNA ranged from 2.1 × 10 3 to 2.6 × 10 4 , 7.4 × 10 2 to 1.4 × 10 3 , 2.5 × 10 2 to 6.4 × 10 3 , and 1.2 × 10 3 to 5.5 × 10 3 , respectively. The densities of 16S rRNA genes per gram of soil increased with progressing soil development. The densities as well as relative abundances of different denitrification genes provide evidence that different denitrifier communities develop under primary succession: higher percentages of narG and nirS versus 16S rRNA genes were observed in the early stage of primary succession, while the percentages of nirK and nosZ genes showed no significant increase or decrease with soil age. Statistical analyses revealed that the amount of organic substances was the most important factor in the abundance of eubacteria as well as of nirK and nosZ communities, and copy numbers of these two genes were the most important drivers changing the denitrifying community along the chronosequence. This study yields an initial insight into the ecology of bacteria carrying genes for the denitrification pathway in a newly developing alpine environment.

Silicon pools and fluxes in soils and landscapes—a review

Abstract: Silicon (Si) is the second-most abundant element in the earth's crust. In the pedosphere, however, huge spans of Si contents occur mainly caused by Si redistribution in soil profiles and landscapes. Here, we summarize the current knowledge on the different pools and fluxes of Si in soils and terrestrial biogeosystems. Weathering and subsequent release of soluble Si may lead to (1) secondarily bound Si in newly formed Al silicates, (2) amorphous silica precipitation on surfaces of other minerals, (3) plant uptake, formation of phytogenic Si, and subsequent retranslocation to soils, (4) translocation within soil profiles and formation of new horizons, or (5) translocation out of soils (desilication). The research carried out hitherto focused on the participation of Si in weathering processes, especially in clay neoformation, buffering mechanisms for acids in soils or chemical denudation of landscapes. There are, however, only few investigations on the characteristics and controls of the low-crystalline, almost pure silica compounds formed during pedogenesis. Further, there is strong demand to improve the knowledge of (micro)biological and rhizosphere processes contributing to Si mobilization, plant uptake, and formation of phytogenic Si in plants, and release due to microbial decomposition. The contribution of the biogenic Si sources to Si redistribution within soil profiles and desilication remains unknown concerning the pools, rates, processes, and driving forces. Comprehensive studies considering soil hydrological, chemical, and biological processes as well as their interactions at the scale of pedons and landscapes are necessary to make up and model the Si balance and to couple terrestrial processes with Si cycle of limnic, fluvial, or marine biogeosystems.

Betalain stability and degradation--structural and chromatic aspects

Abstract: In recent years, food coloring with artificial colorants has been increasingly disapproved by consumers In return, application of coloring foodstuffs, among them betalain-containing fruits and vegetables, has gained importance for the food industry As commonly true for natural pigments, betalains are afflicted with inferior stability compared to synthetic dyes Especially temperature, oxygen, and light are known to exhibit detrimental effects on betalain integrity, while certain antioxidants and chelating agents may act as stabilizers Only recently, several studies expanded the knowledge on betalain degradation pathways, especially focusing on betacyanin decomposition Additionally, new findings on stability and stabilization of betalains in cactus fruit juices extended the application range of betalainic foodstuffs Focusing on betacyanins, the present review discusses betalain degradation mechanisms and provides a survey of compounds and conditions governing betalain stability in a beneficial or an unfavorable way Finally, strategies for maintaining the chromatic properties and tinctorial strength of betalain-based juices and pigment preparations as well as tools for color modulation by targeted betacyanin degradation are discussed

Welfare of broilers: a review

Abstract: Selection for fast early growth rate and feeding and management procedures which support growth have lead to various welfare problems in modern broiler strains. Problems which are directly linked to growth rate are metabolic disorders causing mortality by the Sudden Death Syndrome and ascites. Fast growth rate is generally accompanied by decreased locomotor activity and extended time spent sitting or lying. The lack of exercise is considered a main cause of leg weakness, and extreme durations of sitting on poor quality litter produces skin lesions at the breast and the legs. Management factors which slow down early growth alleviate many welfare problems. Alternatively it may be considered to use slow growing strains which do not have the above mentioned welfare problems. Since growth is a main economical factor, there are problems of acceptability of these measures in the commercial broiler production. Stocking density is a central issue of broiler welfare. It is evident, that the influence of stocking density on growth rate and leg problems acts through its influence on litter and air quality. High moisture content of the litter enhances microbial activity, which in turn leads to increase of temperature and ammonia in broiler houses, and thus, high incidence of contact dermatitis. High stocking density impedes heat transfer from the litter surface to the ventilated room. This restricts the efficacy of conventional ventilation systems in alleviating heat stress. Lighting programmes with reduced photoperiods are considered essential for the stimulation of locomotor activity and the development of a circadian rhythm in the birds. Extended dark periods, however, reduce growth when applied in the first weeks of age. Compensation occurs when the time of the production cycle is substantially increased. Various methods to enrich the environment have shown only moderate effects on the behaviour and physical conditions of broilers.

Santorini eruption radiocarbon dated to 1627-1600 B.C.

Abstract: Precise and direct dating of the Minoan eruption of Santorini (Thera) in Greece, a global Bronze Age time marker, has been made possible by the unique find of an olive tree, buried alive in life position by the tephra (pumice and ashes) on Santorini. We applied so-called radiocarbon wiggle-matching to a carbon-14 sequence of tree-ring segments to constrain the eruption date to the range 1627-1600 B.C. with 95.4% probability. Our result is in the range of previous, less precise, and less direct results of several scientific dating methods, but it is a century earlier than the date derived from traditional Egyptian chronologies.

Fertilization and Nitrogen Balance in a Wheat–Maize Rotation System in North China

Abstract: Over N fertilization is a common problem for the winter wheat (Triticum aestivum L.)-summer maize (Zea mays L.) rotation system in the North China Plain. A field experiment which included control (no N), conventional N (Con. N) fertilization, and optimized N (N min ) fertilization treatments, was conducted from 1999 to 2003 near Beijing, China. Soil nitrate (NO 3 ) dynamics were measured and N balance was calculated for the period of the eight successive cropping seasons. Soil NO 3 -N in the 0- to 90-cm profile for the Con. N treatment ranged from 157 to 700 kg ha -1 during the eight successive cropping seasons, much greater than those in the no N and optimized N treatments. Large amounts of soil NO 3 -N were detected in the 90- to 200-cm layer under the conventional N fertilization treatment, especially in the summer maize season. For the N min treatment, the total amount of N applied was 511 kg N ha -1 in the eight successive crops as compared with 2400 kg N ha -1 of the Con. N treatment. Grain yields were not different between the fertilized treatments except for maize in 2003. Soil NO 3 -N in the root zone under conditions of optimized N fertilization was maintained at a relatively low level as compared with the Con. N treatment, therefore dramatically decreasing NO 3 -N movement to deeper soil profile. This study indicates that soil NO 3 movement out of the effective crop root zone is an important pathway of N losses in this winter wheat-summer maize rotation system in the North China Plain and the optimized N fertilization by an improved N min method shows high potential of reducing N-leaching losses.

Review of methodologies for extracting plant-available and amorphous Si from soils and aquatic sediments

Abstract: There is a variety of methodologies used in the aquatic sciences and soil sciences for extracting different forms of Si from sediments and soils. However, a comparison of the published extraction techniques is lacking. Here we review the methodologies used to extract different Si fractions from soils and sediments. Methods were classified in those to assess plant-available Si and those to extract Si from amorphous silica and allophane. Plant-available Si is supposed to comprise silicic acid in soil solution and adsorbed to soil particles. Extraction techniques for plant-available Si include extractions with water, CaCl2, acetate, acetic acid, phosphate, H2SO3, H2SO4, and citrate. The extractants show different capabilites to desorb silicic acid, with H2SO3, H2SO4 and citrate having the greater extraction potential. The most common extractants to dissolve amorphous silica from soils and aquatic sediments are NaOH and Na2CO3, but both also dissolve crystalline silicates to varying degrees. In soils moreover Tiron is used to dissolve amorphous silica, while oxalate is used to dissolve allophanes and imogolite-type materials. Most techniques analyzing for biogenic silica in aquatic environments use a correction method to identify mineral derived Si. By contrast, in the soil sciences no correction methods are used although pedologists are well aware of the overestimation of amorphous silica by the NaOH extraction, which is most commonly used to extract silica from soils. It is recommended that soil scientists begin to use the techniques developed in the aquatic sciences, since it seems impossible to extract amorphous Si from soils completely without dissolving some of the crystalline silicates.

Involvement of trichothecenes in fusarioses of wheat, barley and maize evaluated by gene disruption of the trichodiene synthase (Tri5) gene in three field isolates of different chemotype and virulence.

Abstract: SUMMARY Fusarium graminearum is the main causative agent of Fusarium head blight on small grain cereals and of ear rot on maize. The disease leads to dramatic yield losses and to an accumulation of mycotoxins. The most dominant F. graminearum mycotoxins are the trichothecenes, with deoxynivalenol and nivalenol being the most prevalent derivatives. To investigate the involvement of trichothecenes in the virulence of the pathogen, the gene coding for the initial enzyme of the trichothecene pathway was disrupted in three field isolates, differing in chemotype and in virulence. From each isolate three individual disruption mutants were tested for their virulence on wheat, barley and maize. Despite the different initial virulence of the three wild-type progenitor strains on wheat, all disruption mutants caused disease symptoms on the inoculated spikelet, but the symptoms did not spread into other spikelets. On barley, the trichothecene deficient mutants showed no significant difference compared to the wild-type strains: all were equally aggressive. On maize, mutants derived from the NIV-producing strain caused less disease than their wild-type progenitor strain, while mutants derived from DON-producing strains caused the same level of disease as their progenitor strains. These data demonstrate that trichothecenes influence the virulence of F. graminearum in a highly complex manner, which is strongly host as well as moderately chemotype specific.

Sources and mechanisms of priming effect induced in two grassland soils amended with slurry and sugar

Abstract: The mechanisms and specific sources of priming effects, ie short term changes of soil organic matter (SOM) decomposition after substance addition, are still not fully understood These uncertainties are partly method related, ie until now only two C sources in released CO2 could be identified We used a novel approach separating three carbon (C) sources in CO2 efflux from soil The approach is based on combination of different substances originated from C3 or C4 plants in different treatments and identical transformation of substances like C3 sugar (from sugar beet) and C4 sugar (from sugar cane) We investigated the influence of the addition of two substances having different microbial utilizability, ie slurry and sugar on the SOM or/and slurry decomposition in two grassland soils with different levels of Corg (23 vs 51% C) Application of slurry to the soil slightly accelerated the SOM decomposition Addition of sugar lead to changes of SOM and slurry decomposition clearly characterized by two phases: immediately after sugar addition, the microorganisms switched from the decomposition of hardly utilizable SOM to the decomposition of easily utilizable sugar This first phase was very short (2‐3 days), hence was frequently missed in other experiments The second phase showed a slightly increased slurry and SOM decomposition (compared to the soil without sugar) The separation of three sources in CO2 efflux from grassland soils allowed us to conclude that the C will be utilized according to its utilizability: sugarOslurryOSOM Additionally, decomposition of more inert C (here SOM) during the period of intensive sugar decomposition was strongly reduced (negative priming effect) We conclude that, priming effects involve a chain of mechanisms: (i) preferential substrate utilization, (ii) activation of microbial biomass by easily utilizable substrate (iii) subsequent increased utilization of following substrates according to their utilizability, and (iv) decline to initial state q 2005 Elsevier Ltd All rights reserved

A Pheromone-Binding Protein Mediates the Bombykol-Induced Activation of a Pheromone Receptor In Vitro

Abstract: The enormous capacity of the male silkmoth Bombyx mori in recognizing and discriminating bombykol and bombykal is based on distinct sensory neurons in the antennal sensilla hairs. The hydrophobic pheromonal compounds are supposed to be ferried by soluble pheromone-binding proteins (PBPs) through the sensillum lymph toward the receptors in the dendritic membrane. We have generated stable cell lines expressing the candidate pheromone receptors of B. mori, BmOR-1 or BmOR-3, and assessed their responses to hydrophobic pheromone compounds dissolved by means of dimethyl sulfoxide. BmOR-1–expressing cells were activated by bombykol but also responded to bombykal, whereas cells expressing BmOR-3 responded to bombykal only. In experiments employing the B. mori PBP, no organic solvent was necessary to mediate an activation of BmOR-1 by bombykol, indicating that the PBP solubilizes the hydrophobic compound. Furthermore, the employed PBP selectively mediated a response to bombykol but not to bombykal, supporting a ligand specificity of PBPs. This study provides evidence that both distinct pheromone receptors and PBPs play an important role in insect pheromone recognition.

Adoption of Bt Cotton and Impact Variability: Insights from India

Abstract: There is a growing body of literature about the impacts of Bacillus thuringiensis (Bt) cotton in developing countries. While many studies show remarkable benefits for farmers, there are also reports that question these results. Most previous studies consider impacts in deterministic terms, neglecting existing variability. Here we explain the main factors influencing the agronomic and economic outcomes. Apart from differences in pest pressure and patterns of pesticide use, germplasm effects can play an important role. Theoretical arguments are supported by empirical evidence from India. Better understanding of impact variability can help explain some of the paradoxes in the recent controversy over genetically modified crops.

Natural occurrence of 16 fusarium toxins in grains and feedstuffs of plant origin from Germany.

Abstract: A total of 220 samples comprising cereals, cereal byproducts, corn plants and corn silage as well as non-grain based feedstuffs was randomly collected during 2000 and 2001 from sources located in Germany and analysed for 16 Fusarium toxins. The trichothecenes scirpentriol (SCIRP), 15-monoacetoxyscirpenol (MAS), diacetoxyscirpenol (DAS), T-2 tetraol, T-2 triol, HT-2 and T-2 toxin (HT-2, T-2), neosolaniol (NEO), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivealenol (15-ADON), nivalenol (NIV) and fusarenon-X (FUS-X) were determined by gas chromatography/mass spectrometry. Zearalenone (ZEA) and α- and β-zearalenol (α- and β-ZOL) were analysed by high performance liquid chromatography with fluorescence and UV-detection. Detection limits ranged between 1 and 19 μg/kg. Out of 125 samples of a group consisting of wheat, oats, corn, corn byproducts, corn plants and corn silage only two wheat samples did not contain any of the toxins analysed. Based on 125 samples the incidences were at 2–11% for DAS, NEO, T-2 Triol, FUS-X, α- and β-ZOL, at 20–22% for SCIRP, MAS, T-2 tetraol and 3-ADON, at 44–74% for HT-2, T-2, 15-ADON, NIV and ZEA, and at 94% for DON. Mean levels of positive samples were between 6 and 758 μg/kg. Out of 95 samples of a group consisting of hay, lupines, peas, soya meal, rapeseed meal and other oilseed meals, 64 samples were toxin negative. DAS, T-2 triol, NEO and FUS-X were not detected in any sample. The incidences of DON and ZEA were at 14 and 23% respectively, those of the other toxins between 1–4%, mean levels of positive samples were between 5 and 95 μg/kg.

Importance of plant species and external silicon concentration to active silicon uptake and transport

Abstract: Summary • Here, we characterized silicon (Si) uptake and xylem loading in Oryza sativa, Zea mays, Helianthus annuus and Benincase hispida in a series of hydroponic experiments. Both active and passive Si-uptake components co-exist in all the plants tested. The active component is the major mechanism responsible for Si uptake in O. sativa and Z. mays. • By contrast, passive uptake prevails in H. annuus and B. hispida at a higher external Si concentration (0.85 mm), while the active component constantly exists and contributes to the total Si uptake, especially at a lower external Si concentration (0.085 mm). • Short experiments showed that Si uptake was significantly suppressed in O. sativa and Z. mays by metabolic inhibitors or low temperature, regardless of external Si concentrations. By contrast, Si uptake in H. annuus and B. hispida was inhibited more significantly by metabolic inhibitors or low temperature at lower (for example, 0.085 mm) than at higher (for example, 1.70 mm) external Si concentrations. • It can be concluded that both active and passive Si-uptake components co-exist in O. sativa, Z. mays, H. annuus and B. hispida, with their relative contribution being dependent much upon both plant species and external Si concentrations.

Influence of the gastrointestinal microbiota on development of the immune system in young animals.

Abstract: The gastrointestinal tract (GIT) of adult mammals is colonized by a complex and dynamic community of microorganisms. Most protection against potential pathogens occurs via a mucosal immune system involving mechanisms of innate immunity as well as a secondary lymphoid organ, the gut-associated lymphoid tissue (GALT). However, the bacterial community also supports its host against invasion by potential pathogens, by a mechanism called 'colonization resistance'. Young animals need time to develop both a complex bacterial community and their immature GIT immune system, and until such developments have taken place, they are vulnerable to the presence of potential pathogens in their GIT. Initial protection against invading pathogens is provided by milk and colostrum, which contain antibodies and other bioactive components. At weaning, with the introduction of solid food and deprivation of the mother's milk, the young must also cope with a rapidly changing microbiota. The colonizing microbiota not only provides colonization resistance to potentially pathogenic bacteria. It also has a major role in the development of the intestinal immune system, both in terms of GALT development and mucosal immunity, and the induction of oral tolerance. Studies using gnotobiotic animal models have revealed that the presence of even limited numbers of the indigenous microbiota may influence the GIT immune system. Regulation of the composition of the GIT microbiota, e.g. by the use of pre- and probiotics, offers the possibility to influence the development of mucosal, and also systemic immunity.

Additive contribution of AMT1;1 and AMT1;3 to high‐affinity ammonium uptake across the plasma membrane of nitrogen‐deficient Arabidopsis roots

Abstract: †‡ Summary In Arabidopsis four root-expressed AMT genes encode functional ammonium transporters, which raises the question of their role in primary ammonium uptake. After pre-culturing under nitrogen-deficiency conditions, we quantified the influx of 15 N-labeled ammonium in T-DNA insertion lines and observed that the loss of either AMT1;1 or AMT1;3 led to a decrease in the high-affinity ammonium influx of approximately 30%. Under nitrogen-sufficient conditions the ammonium influx was lower in Columbia glabra compared with Wassilewskija (WS), and AMT1;1 did not contribute significantly to the ammonium influx in Col-gl. Ectopic expression of AMT1;3 under the control of a 35S promoter in either of the insertion lines amt1;3-1 or amt1;1-1 increased the ammonium influx above the level of their corresponding wild types. In transgenic lines carrying AMT-promoter‐GFP constructs, the promoter activities of AMT1;1 and AMT1;3 were both upregulated under nitrogen-deficiency conditions and were localized to the rhizodermis, including root hairs. AMT gene‐GFP fusions that were stably expressed under the control of their own promoters were localized to the plasma membrane. The double insertion line amt1;1-1 amt1;3-1 showed a decreased sensitivity to the toxic ammonium analog methylammonium and a decrease in the ammonium influx of up to 70% relative to wildtype plants. These results suggest an additive contribution of AMT1;1 and AMT1;3 to the overall ammonium uptake capacity in Arabidopsis roots under nitrogen-deficiency conditions.

Practical experiences with a system for site‐specific weed control in arable crops using real‐time image analysis and GPS‐controlled patch spraying

Abstract: Summary Information on temporal and spatial variation in weed seedling populations within agricultural fields is very important for weed population assessment and management. Primarily, spatial information allows a potential reduction in herbicide use, when post-emergent herbicides are only applied to field sections with high weed infestation levels. This paper presents a system for site-specific weed control in sugar beet, maize, winter wheat, winter barley, winter rape and spring barley. The system includes on-line weed detection using digital image analysis, computer-based decision making and Global Positioning System-controlled patch spraying. In a 2-year study, herbicide use with this map-based approach was reduced in winter cereals by 6–81% for herbicides against broad leaved weeds and 20–79% for grass weed herbicides. Highest savings were achieved in cereals followed by sugar beet, maize and winter rape. The efficacy of weed control varied from 85% to 98%, indicating that site-specific weed management will not result in higher infestation levels in the following crops.

The sense of smell: multiple olfactory subsystems.

Abstract: The mammalian olfactory system is not uniformly organized but consists of several subsystems each of which probably serves distinct functions. Not only are the two major nasal chemosensory systems, the vomeronasal organ and the main olfactory epithelium, structurally and functionally separate entities, but the latter is further subcompartimentalized into overlapping expression zones and projection-related subzones. Moreover, the populations of 'OR37' neurons not only express a unique type of olfactory receptors but also are segregated in a cluster-like manner and generally project to only one receptor-specific glomerulus. The septal organ is an island of sensory epithelium on the nasal septum positioned at the nasoplatine duct; it is considered as a 'mini-nose' with dual function. A specific chemosensory function of the most recently discovered subsystem, the so-called Grueneberg ganglion, is based on the expression of olfactory marker protein and the axonal projections to defined glomeruli within the olfactory bulb. This complexity of distinct olfactory subsystems may be one of the features determining the enormous chemosensory capacity of the sense of smell.

Response of microbial activity and microbial community composition in soils to long-term arsenic and cadmium exposure

Abstract: Arsenic (As) and cadmium (Cd) in soils can affect soil microbial function and community composition and, therefore, may have effects on soil ecosystem functioning. The aim of our study was to assess the effects of long-term As and Cd contamination on soil microbial community composition and soil enzyme activities. We analyzed soils that have been contaminated 25 years ago and at present still show enhanced levels of either As, 18 and 39 mg kg−1, or Cd, 34 and 134 mg kg−1. Soil without heavy metal addition served as control. Polymerase chain reaction (PCR) followed by denaturing gradient gel electrophoresis (DGGE) showed that bacterial community composition in As and Cd contaminated soils differed from that in the control soil. The same was true for the microbial community composition assessed by analysis of respiratory quinones. Soil fungi and Proteobacteria appeared to be tolerant towards As and Cd, while other groups of bacteria were reduced. The decline in alkaline phosphatase, arylsulphatase, protease and urease activities in the As- and Cd-contaminated soils was correlated with a decrease of respiratory quinones occuring in Actinobacteria and Firmicutes. Xylanase activity was unaffected or elevated in the contaminated soils which was correlated with a higher abundance of fungal quinones, and quinones found in Proteobacteria.

Survival of mite infested (Varroa destructor) honey bee (Apis mellifera) colonies in a Nordic climate

Abstract: An isolated honey bee population (N = 150) was established on the southern tip of Gotland, an island in the Baltic sea. After infestation with 36 to 89 Varroa destructor mites per colony, they were unmanaged and allowed to swarm. For over six years colonies were monitored for swarming, winter losses, infestation rate in the fall, and bee population size in the spring. Winter mortality rate decreased from 76% and 57% in the third and fourth years, to 13% and 19% in the fifth and sixth years. Swarming rates increased from zero the third field season to 57.1% and 36.4% in the last two years. The mite infestation on adult bees decreased during the last two years, from 0.47% in the third year to 0.19% and 0.22% respectively. Our data suggest that a host-parasite co-adaptation has occurred ensuring survival of both the host and the parasite. The mechanisms behind this co-adaptation require further study. Varroa destructor / Apis mellifera / host-parasite interaction / survival / adaption

Capturing the complexity of water uses and water users within a multi-agent framework

Abstract: Due to the hydrological and socio-economic complexity of water use within river basins and even sub-basins, it is a considerable challenge to manage water resources in an efficient, equitable and sustainable way. This paper shows that multi-agent simulation (MAS) is a promising approach to better understand the complexity of water uses and water users within sub-basins. This approach is especially suitable to take the collective action into account when simulating the outcome of technical innovation and policy change. A case study from Chile is used as an example to demonstrate the potential of the MAS framework. Chile has played a pioneering role in water policy reform by privatizing water rights and promoting trade in such rights, devolving irrigation management authority to user groups, and privatizing the provision of irrigation infrastructure. The paper describes the different components of a MAS model developed for four micro-watersheds in the Maule river basin. Preliminary results of simulation experiments are presented, which show the impacts of technical change and of informal rental markets on household income and water use efficiency. The paper also discusses how the collective action problems in water markets and in small-scale and large-scale infrastructure provision can be captured by the MAS model. To promote the use of the MAS approach for planning purposes, a collaborative research and learning framework has been established, with a recently created multi-stakeholder platform at the regional level (Comision Regional de Recursos Hidricos) as the major partner. Finally, the paper discusses the potentials of using MAS models for water resources management, such as increasing transparency as an aspect of good governance. The challenges, for example the need to build trust in the model, are discussed as well.

White lupin has developed a complex strategy to limit microbial degradation of secreted citrate required for phosphate acquisition.

Abstract: White lupins (Lupinus albus L.) respond to phosphate deficiency by producing special root structures called cluster roots. These cluster roots secrete large amounts of carboxylates into the rhizosphere, mostly citrate and malate, which act as phosphate solubilizers and enable the plant to grow in soils with sparingly available phosphate. The success and efficiency of such a P-acquisition strategy strongly depends on the persistence and stability of the carboxylates in the soil, a parameter that is influenced to a large extent by biodegradation through rhizosphere bacteria and fungi. In this study, we show that white lupin roots use several mechanisms to reduce microbial growth. The abundance of bacteria associated with cluster roots was decreased at the mature state of the cluster roots, where a burst of organic acid excretion and a drastic pH decrease is observed. Excretion of phenolic compounds, mainly isoflavonoids, induced fungal sporulation, indicating that vegetative growth, and thus potential citrate consumption, is reduced. In addition, the activity of two antifungal cell wall-degrading enzymes, chitinase and glucanase, were highest at the stage preceding the citrate excretion. Therefore, our results suggest that white lupin has developed a complex strategy to reduce microbial degradation of the phosphate-solubilizing agents.

Synchronizing N Supply from Soil and Fertilizer and N Demand of Winter Wheat by an Improved Nmin Method

Abstract: Excessive nitrogen (N) fertilizer application and poor timing of N fertilizer application to winter wheat are common problems on the North China Plain. To study the possibilities of optimizing the timing and rate of N application, a field experiment was conducted from 1999 to 2001 in a suburb of Beijing. A control (no nitrogen) and two N fertilization strategies (conventional N application and optimized N fertilization) were designed to compare their effects on wheat growth, N nutrient status, grain yield and N balance. The conventional N fertilization strategy was given a fixed N rate of 300 kg N ha−1, which was split, half in autumn and half in spring as a top-dressing. The timing and rate of N and application of the optimized N fertilization strategy were determined by the target value of soil mineral nitrogen demand for three growth periods of wheat, which is related to the target yield, and soil mineral N (Nmin) in the effective rooting depth at the beginning of these three periods. Based on the optimized N fertilization strategy, a total of 55 and 65 kg N ha−1 had to be applied to winter wheat in the re-greening and shooting stages of the first and second experimental years, respectively. Compared with the high N rate before sowing in the conventional N fertilization treatment, the optimized N fertilization treatment did not require any N fertilizer before sowing of wheat. Despite a much lower N fertilization rate, no significant difference in N nutrient status, growth during the wheat growing period or grain yield was observed between optimized N and conventional N fertilization treatments. As a consequence of optimizing the rate and timing of the N fertilizer application to match wheat demand, a much lower residual Nmin and calculated apparent N loss was found as compared to the conventional N treatment. N recovery for the optimized N fertilization treatment (67% in 1999/2000 and 66% in 2000/2001) was much higher than that of the conventional N fertilization treatment (19% in 1999/2000 and 18% in 2000/2001). In conclusion, the optimized N fertilization strategy can synchronize N demand of wheat and the N supply from soil and fertilizer, and therefore drastically reduce N application rates without any yield losses.