University of Patras

About: University of Patras is a education organization based out in Pátrai, Greece. It is known for research contribution in the topics: Population & Catalysis. The organization has 13372 authors who have published 31263 publications receiving 677159 citations. The organization is also known as: Panepistímio Patrón.

Suppression of tumor growth and angiogenesis by a specific antagonist of the cell-surface expressed nucleolin.

Abstract: Background: Emerging evidences suggest that nucleolin expressed on the cell surface is implicated in growth of tumor cells and angiogenesis. Nucleolin is one of the major proteins of the nucleolus, but it is also expressed on the cell surface where is serves as a binding protein for variety of ligands implicated in cell proliferation, differentiation, adhesion, mitogenesis and angiogenesis. Methodology/Principal Findings: By using a specific antagonist that binds the C-terminal tail of nucleolin, the HB-19 pseudopeptide, here we show that the growth of tumor cells and angiogenesis are suppressed in various in vitro and in vivo experimental models. HB-19 inhibited colony formation in soft agar of tumor cell lines, impaired migration of endothelial cells and formation of capillary-like structures in collagen gel, and reduced blood vessel branching in the chick embryo chorioallantoic membrane. In athymic nude mice, HB-19 treatment markedly suppressed the progression of established human breast tumor cell xenografts in nude mice, and in some cases eliminated measurable tumors while displaying no toxicity to normal tissue. This potent antitumoral effect is attributed to the direct inhibitory action of HB-19 on both tumor and endothelial cells by blocking and down regulating surface nucleolin, but without any apparent effect on nucleolar nucleolin. Conclusion/Significance: Our results illustrate the dual inhibitory action of HB-19 on the tumor development and the neovascularization process, thus validating the cell-surface expressed nucleolin as a strategic target for an effective cancer drug. Consequently, the HB-19 pseudopeptide provides a unique candidate to consider for innovative cancer therapy.

Scene Detection in Videos Using Shot Clustering and Sequence Alignment

Abstract: Video indexing requires the efficient segmentation of video into scenes. The video is first segmented into shots and a set of key-frames is extracted for each shot. Typical scene detection algorithms incorporate time distance in a shot similarity metric. In the method we propose, to overcome the difficulty of having prior knowledge of the scene duration, the shots are clustered into groups based only on their visual similarity and a label is assigned to each shot according to the group that it belongs to. Then, a sequence alignment algorithm is applied to detect when the pattern of shot labels changes, providing the final scene segmentation result. In this way shot similarity is computed based only on visual features, while ordering of shots is taken into account during sequence alignment. To cluster the shots into groups we propose an improved spectral clustering method that both estimates the number of clusters and employs the fast global k-means algorithm in the clustering stage after the eigenvector computation of the similarity matrix. The same spectral clustering method is applied to extract the key-frames of each shot and numerical experiments indicate that the content of each shot is efficiently summarized using the method we propose herein. Experiments on TV-series and movies also indicate that the proposed scene detection method accurately detects most of the scene boundaries while preserving a good tradeoff between recall and precision.

Perovskite solar cell with low cost Cu-phthalocyanine as hole transporting material

Abstract: Low cost copper phthalocyanine has been used as hole-transporting material for the construction of organolead halide solid state perovskite solar cells. The cells were assembled and tested under ambient conditions. They achieved a power conversion efficiency of 5.0% using copper phthalocyanine, which appears to have potential to replace the currently used organic hole transporters. The present work has also examined the possibility of upscaling by construction of small cell modules.

Electromagnetically induced transparency and controlled group velocity in a multilevel system

Abstract: We analyze the interaction of N laser fields with a $(N+1)$-level quantum system. A general analytic expression for the steady-state linear susceptibility for a probe-laser field is obtained and we show that the system can exhibit multiple electromagnetically induced transparency, with at most $N\ensuremath{-}1$ transparency windows occurring in the system. The group velocity of the probe-laser pulse can also be controlled.

Thrombin promotes endothelial cell alignment in Matrigel in vitro and angiogenesis in vivo

Abstract: We have tested the effect of thrombin on endothelial cell tube formation in vitro and angiogenesis in vivo. Thrombin induces the differentiation of endothelial cells into capillary structures in a ...