2D Differential In-gel Electrophoresis for the Identification of Esophageal Scans Cell Cancer-specific Protein Markers
Ge Zhou,Hongmei Li,Dianne L. DeCamp,She Chen,Hongjun Shu,Yi Gong,Michael J. Flaig,John W. Gillespie,Nan Hu,Philip R. Taylor,Michael R. Emmert-Buck,Lance A. Liotta,Emanuel F. Petricoin,Yingming Zhao +13 more
TLDR
Global quantification of protein expression between laser capture microdissection-microdissected patient-matched cancer cells and normal cells using 2D DIGE in combination with mass spectrometry is a powerful tool for the molecular characterization of cancer progression and identification of cancer-specific protein markers.About:
This article is published in Molecular & Cellular Proteomics.The article was published on 2002-02-01 and is currently open access. It has received 378 citations till now. The article focuses on the topics: Gel electrophoresis & Cancer cell.read more
Citations
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Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics.
Shao En Ong,Blagoy Blagoev,Irina Kratchmarova,Dan B. Kristensen,Hanno Steen,Akhilesh Pandey,Matthias Mann +6 more
TL;DR: SILAC is a simple, inexpensive, and accurate procedure that can be used as a quantitative proteomic approach in any cell culture system and is applied to the relative quantitation of changes in protein expression during the process of muscle cell differentiation.
Journal ArticleDOI
Comparing protein abundance and mRNA expression levels on a genomic scale
Dov Greenbaum,Christopher M. Colangelo,Christopher M. Colangelo,Kenneth R. Williams,Kenneth R. Williams,Mark Gerstein +5 more
TL;DR: This work merges many of the available yeast protein-abundance datasets, using the resulting larger 'meta-dataset' to find correlations between protein and mRNA expression, both globally and within smaller categories.
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Biomarkers in cancer staging, prognosis and treatment selection.
TL;DR: Advances in genomics, proteomics and molecular pathology have generated many candidate biomarkers with potential clinical value, but how and when they can be integrated into clinical care is crucial.
Journal ArticleDOI
Proteomic applications for the early detection of cancer.
TL;DR: Proteomic analyses of early-stage cancers have provided new insights into the changes that occur in the early phases of tumorigenesis and represent a new resource of candidate biomarkers for early stage disease as discussed by the authors.
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Integrated Genomic and Proteomic Analyses of Gene Expression in Mammalian Cells
Qiang Tian,Serguei B. Stepaniants,Mao Mao,Lee Weng,Megan C. Feetham,Michelle J. Doyle,Eugene C. Yi,Hongyue Dai,Vesteinn Thorsson,Jimmy K. Eng,David R. Goodlett,Joel P. Berger,Bert Gunter,Peter S. Linseley,Roland Stoughton,Ruedi Aebersold,Steven J. Collins,William A. Hanlon,Leroy Hood +18 more
TL;DR: Although the overall pattern of protein expression is similar to that of mRNA expression, the incongruent expression between mRNAs and proteins emphasize the importance of posttranscriptional regulatory mechanisms in cellular development or perturbation that can be unveiled only through integrated analyses of both proteins and m RNAs.
References
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High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
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Correlation between Protein and mRNA Abundance in Yeast
TL;DR: The results clearly delineate the technical boundaries of current approaches for quantitative analysis of protein expression and reveal that simple deduction from mRNA transcript analysis is insufficient to predict protein expression levels from quantitative mRNA data.
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Laser Capture Microdissection
Michael R. Emmert-Buck,Robert F. Bonner,Paul D. Smith,Rodrigo F. Chuaqui,Zhengping Zhuang,Seth R. Goldstein,Rhonda A. Weiss,Lance A. Liotta +7 more
TL;DR: Laser capture microdissection under direct microscopic visualization permits rapid one-step procurement of selected human cell populations from a section of complex, heterogeneous tissue.
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The current state of two-dimensional electrophoresis with immobilized pH gradients.
Angelika Görg,Christian Obermaier,Günther Boguth,Alois Harder,Burghardt Scheibe,Robert Wildgruber,Walter Weiss +6 more
TL;DR: The original protocol of two-dimensional electrophoresis with immobilized pH gradient (IPG-Dalt) is updated in this article, and the results with extended separation distances as well as automated procedures are demonstrated, and a comparison between protein detection by silver staining and fluorescent dyes is given.
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Difference gel electrophoresis. A single gel method for detecting changes in protein extracts
TL;DR: DIGE is reproducible, sensitive, and can detect an exogenous difference between two Drosophila embryo extracts at nanogram levels and was detected after 15 min of induction and identified using DIGE preparatively.