A modular and compact portable mini-endstation for high-precision, high-speed fixed target serial crystallography at FEL and synchrotron sources
D.A. Sherrell,Andrew Foster,Lee Hudson,Brian Nutter,James O'Hea,Silke Nelson,Olivier Paré-Labrosse,Saeed Oghbaey,R. J. Dwayne Miller,Robin L. Owen +9 more
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TLDR
A portable sample viewing and alignment system is described which provides fast and reliable motion positioning for fixed target arrays at synchrotrons and free-electron laser sources.Abstract:
The design and implementation of a compact and portable sample alignment system suitable for use at both synchrotron and free-electron laser (FEL) sources and its performance are described. The system provides the ability to quickly and reliably deliver large numbers of samples using the minimum amount of sample possible, through positioning of fixed target arrays into the X-ray beam. The combination of high-precision stages, high-quality sample viewing, a fast controller and a software layer overcome many of the challenges associated with sample alignment. A straightforward interface that minimizes setup and sample changeover time as well as simplifying communication with the stages during the experiment is also described, together with an intuitive naming convention for defining, tracking and locating sample positions. The setup allows the precise delivery of samples in predefined locations to a specific position in space and time, reliably and simply.read more
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Journal ArticleDOI
High-speed fixed-target serial virus crystallography.
Philip Roedig,Helen M. Ginn,Tim Pakendorf,Geoff Sutton,Karl Harlos,Thomas S. Walter,Jan Meyer,Pontus Fischer,Ramona Duman,Ismo Vartiainen,Bernd Reime,Martin Warmer,Aaron S. Brewster,Iris D. Young,Tara Michels-Clark,Nicholas K. Sauter,Abhay Kotecha,J. Kelly,David J. Rowlands,Marcin Sikorsky,Silke Nelson,Daniel S. Damiani,Roberto Alonso-Mori,Jingshan Ren,Elizabeth E. Fry,Christian David,David I. Stuart,Armin Wagner,Alke Meents +28 more
TL;DR: Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin are determined.
Journal ArticleDOI
Low-dose fixed-target serial synchrotron crystallography
Robin L. Owen,Danny Axford,D.A. Sherrell,A. Kuo,Oliver P. Ernst,Eike C. Schulz,R.J.D. Miller,R.J.D. Miller,Henrike M. Mueller-Werkmeister,Henrike M. Mueller-Werkmeister +9 more
TL;DR: A time- and sample-efficient approach for the serial collection of room-temperature diffraction data using a fixed target at a synchrotron is demonstrated.
Journal ArticleDOI
Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering
Philip Roedig,Ramona Duman,Juan Sanchez-Weatherby,Ismo Vartiainen,Anja Burkhardt,Martin Warmer,Christian David,Armin Wagner,Alke Meents +8 more
TL;DR: A micro-patterned sample holder of single-crystalline silicon, loaded with multiple protein crystals which are exposed to a humidified gas stream, allows high-quality room-temperature data collection.
Journal ArticleDOI
Strategies for sample delivery for femtosecond crystallography
TL;DR: Strategies for sample delivery of macromolecular crystals at X-ray free-electron lasers are reviewed, covering injection methods, fixed-target approaches and hybrid methods.
References
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Journal ArticleDOI
Femtosecond X-ray protein nanocrystallography
Henry N. Chapman,Petra Fromme,Anton Barty,Thomas A. White,Richard A. Kirian,Andrew Aquila,Mark S. Hunter,Joachim Schulz,Daniel P. DePonte,Uwe Weierstall,R. Bruce Doak,Filipe R. N. C. Maia,Andrew V. Martin,Ilme Schlichting,Lukas Lomb,Nicola Coppola,Robert L. Shoeman,Sascha W. Epp,Robert Hartmann,Daniel Rolles,Artem Rudenko,Lutz Foucar,Nils Kimmel,Georg Weidenspointner,Peter Holl,Mengning Liang,Miriam Barthelmess,Carl Caleman,Sébastien Boutet,Michael J. Bogan,Jacek Krzywinski,Christoph Bostedt,Saša Bajt,Lars Gumprecht,Benedikt Rudek,Benjamin Erk,Carlo Schmidt,André Hömke,Christian Reich,Daniel Pietschner,Lothar Strüder,Günter Hauser,H. Gorke,Joachim Ullrich,Sven Herrmann,Gerhard Schaller,Florian Schopper,Heike Soltau,Kai-Uwe Kühnel,Marc Messerschmidt,John D. Bozek,Stefan P. Hau-Riege,Matthias Frank,Christina Y. Hampton,Raymond G. Sierra,Dmitri Starodub,Garth J. Williams,Janos Hajdu,Nicusor Timneanu,M. Marvin Seibert,M. Marvin Seibert,Jakob Andreasson,Andrea Rocker,Olof Jönsson,Martin Svenda,Stephan Stern,Karol Nass,Robert Andritschke,Claus Dieter Schröter,Faton Krasniqi,Mario Bott,Kevin Schmidt,Xiaoyu Wang,Ingo Grotjohann,James M. Holton,Thomas R. M. Barends,Richard Neutze,Stefano Marchesini,Raimund Fromme,Sebastian Schorb,Daniela Rupp,M. Adolph,Tais Gorkhover,Inger Andersson,Helmut Hirsemann,Guillaume Potdevin,Heinz Graafsma,Björn Nilsson,John C. H. Spence +88 more
TL;DR: This work offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage, by using pulses briefer than the timescale of most damage processes.
Journal ArticleDOI
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
Uwe Weierstall,Daniel James,Chong Wang,Thomas A. White,Dingjie Wang,Wei Liu,John C. H. Spence,R. Bruce Doak,Garrett Nelson,Petra Fromme,Raimund Fromme,Ingo Grotjohann,Christopher Kupitz,Nadia A. Zatsepin,Haiguang Liu,Shibom Basu,Daniel Wacker,Gye Won Han,Vsevolod Katritch,Sébastien Boutet,Marc Messerschmidt,Garth J. Williams,Jason E. Koglin,M. Marvin Seibert,M. Marvin Seibert,Markus Klinker,Cornelius Gati,Robert L. Shoeman,Anton Barty,Henry N. Chapman,Richard A. Kirian,Kenneth R. Beyerlein,Raymond C. Stevens,Dianfan Li,Syed T. A. Shah,Nicole Howe,Martin Caffrey,Vadim Cherezov +37 more
TL;DR: This work presents a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography.
Journal ArticleDOI
Structure of class B GPCR corticotropin-releasing factor receptor 1
Kaspar Hollenstein,James Kean,Andrea Bortolato,Robert K. Y. Cheng,Andrew S. Doré,Ali Jazayeri,Robert M. Cooke,Malcolm Peter Weir,Fiona H. Marshall +8 more
TL;DR: The crystal structure of the transmembrane domain of the human corticotropin-releasing factor receptor type 1 in complex with the small-molecule antagonist CP-376395 is reported and provides detailed insight into the architecture of class B receptors.
Journal ArticleDOI
Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL
Kunio Hirata,Kyoko Shinzawa-Itoh,Naomine Yano,Shuhei Takemura,Koji Kato,Koji Kato,Miki Hatanaka,Kazumasa Muramoto,Takako Kawahara,Tomitake Tsukihara,Tomitake Tsukihara,Eiki Yamashita,Kensuke Tono,Go Ueno,Takaaki Hikima,Hironori Murakami,Yuichi Inubushi,Makina Yabashi,Tetsuya Ishikawa,Masaki Yamamoto,Takashi Ogura,Hiroshi Sugimoto,Jian Ren Shen,Shinya Yoshikawa,Hideo Ago +24 more
TL;DR: The performance of the femtosecond crystallography method is demonstrated by determining a 1.9-Å radiation damage–free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.
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Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
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