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Open AccessJournal ArticleDOI

A more efficient method to generate integration-free human iPS cells

Keisuke Okita, +15 more
- 01 May 2011 - 
- Vol. 8, Iss: 5, pp 409-412
TLDR
A simple method is reported, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors, which may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.
Abstract: 
Human induced pluripotent stem cells are generated with episomal plasmid vectors at increased efficiency using non-transforming L-Myc and knockdown of p53 Also in this issue, Chen et al report defined conditions for human cell reprogramming and culture

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Chemically defined generation of human cardiomyocytes

Paul W. Burridge, +14 more
- 01 Aug 2014 - 
TL;DR: This work systematically developed an optimized cardiac differentiation strategy, using a chemically defined medium consisting of just three components: the basal medium RPMI 1640, L-ascorbic acid 2-phosphate and rice-derived recombinant human albumin, which was effective in 11 hiPSC lines tested.
Journal ArticleDOI

Induced pluripotent stem cell technology: a decade of progress

Yanhong Shi, +3 more
- 01 Feb 2017 - 
TL;DR: The progress in applications of iPSC technology that are particularly relevant to drug discovery and regenerative medicine are discussed, and the remaining challenges and the emerging opportunities in the field are considered.
Journal ArticleDOI

FOXG1-Dependent Dysregulation of GABA/Glutamate Neuron Differentiation in Autism Spectrum Disorders

Jessica Mariani, +15 more
- 16 Jul 2015 - 
TL;DR: Three-dimensional neural cultures derived from induced pluripotent stem cells are used to investigate neurodevelopmental alterations in individuals with severe idiopathic ASD and show that overexpression of the transcription factor FOXG1 is responsible for the overproduction of GABAergic neurons.
Journal ArticleDOI

Induced Pluripotent Stem Cells: Past, Present, and Future

Shinya Yamanaka
- 14 Jun 2012 - 
TL;DR: The development of iPSCs reflected the merging of three major scientific streams and has in turn led to additional new branches of investigation, but there is still debate about whetheriPSCs are functionally equivalent to ESCs.
Journal ArticleDOI

GGGGCC repeat expansion in C9orf72 compromises nucleocytoplasmic transport

Brian D. Freibaum, +13 more
- 03 Sep 2015 - 
TL;DR: It is shown that a primary consequence of G4C2 repeat expansion is the compromise of nucleocytoplasmic transport through the nuclear pore, revealing a novel mechanism of neurodegeneration.
References
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Journal ArticleDOI

Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors

Kazutoshi Takahashi, +6 more
- 30 Nov 2007 - 
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI

Efficient selection for high-expression transfectants with a novel eukaryotic vector

Hitoshi Niwa, +2 more
- 15 Dec 1991 - 
TL;DR: The results showed that high concentrations of G418 efficiently yielded L cell and CHO cell transfectants stably producing IL-2 at levels comparable with those previously attained using gene amplification.
Journal ArticleDOI

Generation of germline-competent induced pluripotent stem cells

Keisuke Okita, +2 more
- 19 Jul 2007 - 
TL;DR: iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.
Journal ArticleDOI

Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA

Luigi Warren, +22 more
- 05 Nov 2010 - 
TL;DR: It is shown that this approach can reprogram multiple human cell types to pluripotency with efficiencies that greatly surpass established protocols and represents a safe, efficient strategy for somatic cell reprogramming and directing cell fate that has broad applicability for basic research, disease modeling, and regenerative medicine.
Journal ArticleDOI

Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences

Junying Yu, +10 more
- 08 May 2009 - 
TL;DR: Results demonstrate that reprograming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells.
Related Papers (5)

Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors

Kazutoshi Takahashi, +6 more
- 30 Nov 2007 - 

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.

Kazutoshi Takahashi, +1 more
- 25 Aug 2006 - 

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Junying Yu, +11 more
- 21 Dec 2007 - 

Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA

Luigi Warren, +22 more
- 05 Nov 2010 - 

Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences

Junying Yu, +10 more
- 08 May 2009 - 
Frequently Asked Questions (1)
Q1. What is the author's affiliation with the study?

This study was supported in part by a grant from the Program forPromotion of Fundamental Studies in Health Sciences of National Institute of Biomedical Innovation,a grant from the Leading Project of Ministry of Education, Culture, Sports, Science and Technology(MEXT), a grant from Funding Program for World-Leading Innovative Research and Development onScience and Technology (FIRST Program) of Japan Society for the Promotion of Science,Grants-in-Aid for Scientific Research of Japan Society for the Promotion of Science and MEXT (toS.Y.), Grant-in-Aid for Young Scientists B (to K.O) and Senri Life Science Foundation (to K.O).Â