Showing papers in "Developmental Cell in 2014"

TL;DR: It is shown that senescent fibroblasts and endothelial cells appear very early in response to a cutaneous wound, where they accelerate wound closure by inducing myofibroblast differentiation through the secretion of platelet-derived growth factor AA (PDGF-AA).

TL;DR: The upstream regulators of myogenesis that lead to the activation of myogenic determination genes and subsequent differentiation, focusing on the mouse model, are discussed, with new insights into mechanisms underlying the transcriptional activity of these factors.

TL;DR: The results uncover a broad expression profile of Osterix and raise the intriguing possibility that distinct waves of stromal cells, primitive and definitive, may organize the developing BM.

TL;DR: This review of classic work and recent progress in studies of centromeric chromatin on vertebrates considers possible roles for repetitive DNA sequences found at most centromeres, chromatin factors and modifications that assemble and activate CENP-A chromatin for Kinetochore assembly, plus the use of artificial chromosomes and kinetochores to study centromere function.

TL;DR: Critically, it is shown that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP, and the absence of one causes expansion of the other, and elimination of both is lethal.

TL;DR: A contact site named vCLAMP is identified that integrates mitochondria with the lysosome-like vacuole and thus the endocytic pathway and Thus, the persistence of vCLAMPs is regulated by phosphorylation of Vps39 and is strongly reduced during respiratory growth.

TL;DR: Biogenesis events are enriched distally; autophagosomes form infrequently in dendrites, the soma, or midaxon, consistent with a compartmentalized pathway for constitutive autophagy in primary neurons.

TL;DR: RNA immunoprecipitation assays demonstrate that AtNSRs interact not only with their alternatively spliced mRNA targets but also with the ASCO-RNA in vivo, and lncRNA can hijack nuclear AS regulators to modulate AS patterns during development.

TL;DR: In this paper, the effect of Tet1/2/3 triple-knockout (TKO) mouse embryonic stem cells (ESCs) on ESC development was examined.

TL;DR: Cyclin B1/Cdk1-mediated phosphorylation of mitochondrial substrates allows cells to sense and respond to increased energy demand for G2/M transition and, subsequently, to upregulate mitochondrial respiration for successful cell-cycle progression.

TL;DR: It is established that TRIMs can function both as regulators of autophagy and as autophagic cargo receptors, and reveals a basis for selective autophapy in mammalian cells.

TL;DR: A brief overview of early cytokinesis events in animal cells is provided and in depth recently emerging models for the assembly and function of the abscission machinery and its temporal coordination with chromosome segregation are covered.

TL;DR: It is shown that CAMSAP2 is required for the proper organization and stabilization of interphase microtubules and directional cell migration and that katanin regulates this process.

TL;DR: It is reported that MeCP2 regulates gene expression posttranscriptionally by suppressing nuclear microRNA processing by binding directly to DiGeorge syndrome critical region 8 (DGCR8), a critical component of the nuclear micro RNA-processing machinery, and interferes with the assembly of Drosha and DG CR8 complex.

TL;DR: To identify Rab effectors, a comprehensive set of Drosophila Rabs was used for affinity chromatography followed by mass spectrometry to identify the proteins bound to each Rab, which confirmed over 25 interactions with known components of membrane traffic as well as with diverse proteins not previously linked to organelles or having no known function.

TL;DR: This work generates Ift25 and Ift27 knockout mice and shows that they have structural birth defects indicative of hedgehog signaling dysfunction, and it is likely that Lztfl1 functions downstream of IFT27 to couple the BBSome to the IFT particle for coordinated removal of patched-1 and Smoothened from cilia during Hedgehog signaling.

TL;DR: A comprehensive, quantitative, multiscale analysis of mammalian kidney development in which changes in cell number, compartment volumes, and cellular dynamics across the entirety of organogenesis are measured, focusing on two key nephrogenic progenitor populations: the ureteric epithelium and the cap mesenchyme.

TL;DR: In this article, the authors extend a recently developed technique, based on deep paired-end sequencing of lightly digested chromatin, to assess footprints of nucleosomes and other DNA-binding proteins genome-wide in murine ESCs and sperm.

TL;DR: Gpr124 functions as a coactivator of Wnt7a- and Wnt 7b-stimulated canonical Wnt signaling via a Frizzled receptor and Lrp coreceptor and that Gpr124- Stimulated signaling functions in concert with Norrin/Frizzled4 signaling to control CNS vascular development.

TL;DR: This study finds that Gata6 mutants exhibit a complete absence of PrE and demonstrates that GATA6 levels regulate the timing and speed of lineage commitment within the ICM, and establishes GATA 6 as a nodal point in the gene regulatory network driving ICM lineage specification.

TL;DR: Evidence that the Hippo pathway effector Yap is a key regulator of this process in the developing lung is provided, showing that when epithelial tubules are forming and branching, a nucleocytoplasmic shift in Yap localization marks the boundary between the airway and the distal lung compartments.

TL;DR: It is shown that, in response to elevated copper, ATP7B moves from the Golgi to lysosomes and imports metal into their lumen and Activation of lysOSomal exocytosis stimulates copper clearance from the hepatocytes and rescues the most frequent Wilson-disease-causing ATP6B mutant to the appropriate functional site.

TL;DR: This Review presents a comparison of the glial response to injury between the CNS and PNS and highlights features of the PNSglial response that, with continued study, might reveal long-sought-after keys to achieving CNS repair.

TL;DR: This work generates a complete 4D map of early Arabidopsis embryogenesis and used computational analysis to demonstrate that several divisions follow a rule that uses the smallest wall area going through the center of the cell.

TL;DR: An unsuspected role of Notch signaling in regulating trophectoderm-specific expression of Cdx2 in cooperation with TEAD4 is shown, which shows that multiple signaling inputs at preimplantation stages specify the first embryonic lineages.

TL;DR: The importance of membrane shape for targeting transmembrane proteins and a method for determining the effective shape and flexibility of membrane proteins are established.

TL;DR: Dual mechanisms prevent aberrant activation of kinesin to ensure robust retrograde transport of autophagosomes along the axon and competitively disrupts JIP1-mediated activation ofKinesin.

TL;DR: It is proposed that FOA serves to select oocytes with limited L1 activity that are therefore best suited for the next generation and reverse transcriptase (RT) inhibitor AZT has a profound effect on the FOA dynamics and meiotic recombination.

TL;DR: It is shown that efficient mitophagy depends on mitochondrial ER tethering and affects the formation of the isolation membrane that engulfs the organelles destined for degradation, providing insights into the cellular mechanisms that govern organelle homeostasis.

TL;DR: In vivo fate mapping of adult hair follicle dermal sheath cells was performed and found that a subset of DS cells are retained following each hair cycle, exhibit self-renewal, and repopulate the DS and the DP with new cells.