An immune clock of human pregnancy.
Nima Aghaeepour,Edward A. Ganio,David R. McIlwain,Amy S. Tsai,Martha Tingle,Sofie Van Gassen,Dyani Gaudilliere,Quentin Baca,Leslie McNeil,Robin Okada,Mohammad Sajjad Ghaemi,David Furman,David Furman,Ronald J. Wong,Virginia D. Winn,Maurice L. Druzin,Yaser Y. El-Sayed,Cecele C. Quaintance,Ronald S. Gibbs,Gary L. Darmstadt,Gary M. Shaw,David K. Stevenson,Robert Tibshirani,Garry P. Nolan,David B. Lewis,Martin S. Angst,Brice Gaudilliere +26 more
TLDR
These findings unravel the precise timing of immunological events occurring during a term pregnancy and provide the analytical framework to identify immunological deviations implicated in pregnancy-related pathologies.Abstract:
The maintenance of pregnancy relies on finely tuned immune adaptations. We demonstrate that these adaptations are precisely timed, reflecting an immune clock of pregnancy in women delivering at term. Using mass cytometry, the abundance and functional responses of all major immune cell subsets were quantified in serial blood samples collected throughout pregnancy. Cell signaling-based Elastic Net, a regularized regression method adapted from the elastic net algorithm, was developed to infer and prospectively validate a predictive model of interrelated immune events that accurately captures the chronology of pregnancy. Model components highlighted existing knowledge and revealed previously unreported biology, including a critical role for the interleukin-2-dependent STAT5ab signaling pathway in modulating T cell function during pregnancy. These findings unravel the precise timing of immunological events occurring during a term pregnancy and provide the analytical framework to identify immunological deviations implicated in pregnancy-related pathologies.read more
Citations
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Integrated Proteotranscriptomics of Human Myometrium in Labor Landscape Reveals the Increased Molecular Associated With Inflammation Under Hypoxia Stress
Lina Chen,Lina Chen,Lele Wang,Yihong Luo,Qian Huang,Kaiyuan Ji,Junjie Bao,Huishu Liu,Huishu Liu +8 more
TL;DR: In this article, the global transcriptome and proteome of human myometrial samples in labor and those not in labor were investigated through RNA sequencing (RNA-seq) and quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) via data-independent acquisition (DIA) and parallel reaction monitoring (PRM) methods.
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CytoGLMM: Conditional Differential Analysis for Flow and Mass Cytometry Experiments
Christof Seiler,Christof Seiler,Anne-Maud Ferreira,Lisa M. Kronstad,Lisa M. Kronstad,Laura J. Simpson,Mathieu Le Gars,Elena Vendrame,Catherine A. Blish,Susan Holmes +9 more
TL;DR: The approach to find differential proteins in flow and mass cytometry data reduces biases arising from maker correlations and safeguards against false discoveries induced by patient heterogeneity.
Journal ArticleDOI
Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells Provides A Protective Microenvironment at The Feto-Maternal Interface: Improved Pregnancy Outcome in Abortion-Prone Mice.
TL;DR: The results indicated that the administration of MSCs, at the implantation window, could significantly decrease the abortion rate and besides, increase the frequency of uterine DCs, a potential mechanism for the improvement of pregnancy outcome.
Journal ArticleDOI
Associations of first trimester co-use of tobacco and Cannabis with prenatal immune response and psychosocial well-being.
Kristin Ashford,Amanda Fallin-Bennett,Andrea McCubbin,Amanda T. Wiggins,Sheila Barnhart,Joshua A. Lile +5 more
TL;DR: Results suggest women co-using during the first trimester exhibit decreased pro-inflammatory immune responsivity on one out of eight markers, and further research is needed to determine the impact of this immune modulation on fetal health outcomes and the unique contribution of cannabis.
Journal ArticleDOI
Enabling precision medicine in neonatology, an integrated repository for preterm birth research
Marina Sirota,Cristel G. Thomas,Rebecca Liu,Maya Zuhl,Payal Banerjee,Ronald J. Wong,Cecele C. Quaintance,Rita Leite,Jessica Chubiz,Rebecca Anderson,Joanne Chappell,Mara Kim,William A. Grobman,Ge Zhang,Antonis Rokas,Sarah K. England,Samuel Parry,Gary M. Shaw,Joe Leigh Simpson,Elizabeth Thomson,Atul J. Butte +20 more
TL;DR: The March of Dimes’ Database for Preterm Birth Research is described, a unique resource that contains a variety of ‘omics’ datasets related to preterm birth that links 13 molecular studies with data across tens of thousands of patients from 6 measurement modalities.
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