Journal ArticleDOI
Analysis of aptamer discovery and technology
Matthew R. Dunn,Randi M. Jimenez,John C. Chaput +2 more
- Vol. 1, Iss: 10, pp 0076
TLDR
A critical analysis of the first 25 years of aptamer research is provided to provide suggestions for choosing chemical modifications that can lead to enhanced activity or stability, and propose standards for the characterization of aptamers in the scientific literature.Abstract:
Aptamers are nucleic acid molecules that mimic antibodies by folding into complex 3D shapes that bind to specific targets. Although some aptamers exist naturally as the ligand-binding elements of riboswitches, most are generated in vitro and can be tailored for a specific target. Relative to antibodies, aptamers benefit from their ease of generation, low production cost, low batch-to-batch variability, reversible folding properties and low immunogenicity. However, the true value of aptamers lies in the simplicity by which these molecules can be engineered into sensors, actuators and other devices that are often central to emerging technologies. This Review examines changing trends in aptamer technology by analysing the first quarter century of aptamer data that is available in the scientific literature (1990–2015). We highlight specific examples that showcase the use of aptamers in key applications, discuss challenges that have impeded the success of aptamers in practical applications, provide suggestions for choosing chemical modifications that can lead to enhanced activity or stability, and propose standards for the characterization of aptamers in the scientific literature. Aptamers are nucleic acid molecules that can be evolved to bind to specific molecular targets and have found applications in technologies such as sensors and actuators. This Review provides a critical analysis of the first 25 years of aptamer research.read more
Citations
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Journal ArticleDOI
Aptamer-based targeted therapy.
Guizhi Zhu,Xiaoyuan Chen +1 more
TL;DR: This review will focus on recent progress and discuss the challenges and opportunities in the research areas ofaptamer‐based targeted therapy in the forms of aptamer therapeutics and aptamer‐drug conjugates (ApDCs).
Journal ArticleDOI
Nucleic Acid Aptamers for Molecular Diagnostics and Therapeutics: Advances and Perspectives.
Long Li,Shujuan Xu,Shujuan Xu,He Yan,He Yan,Xiaowei Li,Hoda Safari Yazd,Xiang Li,Tong Huang,Cheng Cui,Cheng Cui,Jian-Hui Jiang,Weihong Tan,Weihong Tan,Weihong Tan +14 more
TL;DR: This review will focus on the novel applications of aptamers and SELEX, as well as opportunities to develop molecular tools able to meet future clinical needs in biomedicine.
Journal ArticleDOI
Internet of medical things (IoMT)-integrated biosensors for point-of-care testing of infectious diseases.
Shikha Jain,Monika Nehra,Rajesh Kumar,Neeraj Dilbaghi,Tony Y. Hu,Sandeep Kumar,Ajeet Kaushik,Chen-Zhong Li,Chen-Zhong Li +8 more
TL;DR: The recently developed POC diagnostics integrated or future possibilities of integration with IoMT are discussed with focus on emerging and re-emerging infectious diseases like malaria, dengue fever, influenza A (H1N1), human papilloma virus, Ebola virus disease (EVD), Zika virus (ZIKV), and coronavirus (COVID-19).
Journal ArticleDOI
SELEX methods on the road to protein targeting with nucleic acid aptamers.
Payam Bayat,Rahim Nosrati,Mona Alibolandi,Houshang Rafatpanah,Khalil Abnous,Mostafa Khedri,Mohammad Ramezani +6 more
TL;DR: This review will discuss the recent advances in SELEX methods and their advantages and limitations and aptamer applications are briefly outlined in this review.
Journal ArticleDOI
Circular RNAs: Characterization, cellular roles, and applications
Chu-Xiao Liu,Ling-Ling Chen +1 more
TL;DR: Most circular RNAs are produced from the back-splicing of exons of precursor mRNAs as discussed by the authors , and they can serve as templates for translation in different biological and pathophysiological contexts.
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Journal ArticleDOI
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase
Craig Tuerk,Larry Gold +1 more
TL;DR: High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species.
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In vitro selection of RNA molecules that bind specific ligands.
TL;DR: Subpopulations of RNA molecules that bind specifically to a variety of organic dyes have been isolated from a population of random sequence RNA molecules.
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