Journal ArticleDOI
Assessment of the Fecal Lactobacilli Population in Patients with Hepatitis B Virus-Related Decompensated Cirrhosis and Hepatitis B Cirrhosis Treated with Liver Transplant
TLDR
The findings on the frequency of lactobacilli population suggested decreased diversity in groups LC and LT (compared with the healthy controls), and patients tended to have less complex fecal lactabacilli composition than thehealthy controls, especially in the group LC.Abstract:
This study aims to provide an overview of the diversity of intestinal Lactobacillus among Chinese patients with hepatitis B virus (HBV)-related decompensated cirrhosis and who received liver transplant for hepatitis B cirrhosis. Fecal samples were collected from 38 healthy volunteers, 61 patients with HBV-related decompensated cirrhosis (group LC) and 74 patients who had liver transplant for hepatitis B cirrhosis (group LT). Quantitative polymerase chain reaction technology with species-specific primers was applied to investigate lactobacilli 16S rDNA in crude DNA, extracted from fecal samples. Software package Statistical Package for the Social Sciences and Palaeontological Statistics for Windows was used to analyze the data. Lactobacilli population of the two patient groups was different from the healthy control subjects, principal differences being marked decrease in the population of Lactobacillus rhamnosus (p < 0.001 for both patient groups) and reduction in the frequency of Lactobacillus fermentus (p < 0.001 for group LC and p < 0.01 for group LT). Our findings on the frequency of lactobacilli population suggested decreased diversity in groups LC and LT (compared with the healthy controls (p < 0.001 and p < 0.01, respectively)). Patients tended to have less complex fecal lactobacilli composition than the healthy controls, especially in the group LC.read more
Citations
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Journal ArticleDOI
Interactions between the intestinal microbiome and liver diseases.
Bernd Schnabl,David A. Brenner +1 more
TL;DR: The contribution of the intestinal microbiome to liver disease goes beyond simple translocation of bacterial products that promote hepatic injury and inflammation and is reviewed to ensure that microbial metabolites produced in a dysbiotic intestinal environment and host factors are equally important in the pathogenesis of liver disease.
Journal ArticleDOI
The links between the gut microbiome and non-alcoholic fatty liver disease (NAFLD)
Zahra Safari,Philippe Gérard +1 more
TL;DR: The manipulation of the gut microbiome by probiotics, prebiotics or synbiotics was shown to improve liver phenotype in NAFLD patients as well as in rodent models, suggesting further knowledge about the interactions among dysbiosis, environmental factors, and diet and their impacts on the gut–liver axis can improve the treatment of this life-threatening liver disease.
Journal ArticleDOI
Supplementation of Saturated Long-Chain Fatty Acids Maintains Intestinal Eubiosis and Reduces Ethanol-induced Liver Injury in Mice
Peng Chen,Manolito Torralba,Justin Tan,Mallory Embree,Karsten Zengler,Peter Stärkel,Jan-Peter van Pijkeren,Jessica DePew,Rohit Loomba,Samuel B. Ho,Samuel B. Ho,Jasmohan S. Bajaj,Ece Mutlu,Ali Keshavarzian,Hidekazu Tsukamoto,Karen E. Nelson,Derrick E. Fouts,Bernd Schnabl +17 more
TL;DR: In humans and mice, alcohol causes intestinal dysbiosis, reducing the capacity of the microbiome to synthesize saturated LCFA and the proportion of Lactobacillus species, which might reduce ethanol-induced liver injury in patients with alcoholic liver disease.
Journal ArticleDOI
Human Microbiome and its Association With Health and Diseases
Asmaa Althani,Asmaa Althani,Hany E. Marei,Wedad S. Hamdi,Gheyath K. Nasrallah,Mohamed E. El Zowalaty,Souhaila Al Khodor,Maha Al-Asmakh,Hassan Abdel-Aziz,Carlo Cenciarelli +9 more
TL;DR: The present review outlines recent studies devoted to elucidate the possible link between the microbiota and various type of diseases and highlights the potential utilization of microbiota as a potential therapeutic option to treat a wide array of human diseases.
Journal ArticleDOI
The intestinal microbiome and the leaky gut as therapeutic targets in alcoholic liver disease
TL;DR: A review highlights vital events in ALD such as BT, the importance of Toll-like receptor (TLR) signaling, intestinal bacterial overgrowth (IBO), and changes in the intestinal microbiome, as well as possible future options of therapy for ALD modifying the enteric microbiome.
References
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Journal ArticleDOI
The gut flora as a forgotten organ
Ann M O'Hara,Fergus Shanahan +1 more
TL;DR: The flora has a collective metabolic activity equal to a virtual organ within an organ, and the mechanisms underlying the conditioning influence of the bacteria on mucosal homeostasis and immune responses are beginning to be unravelled.
Journal ArticleDOI
Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction
TL;DR: Analysis of melting curves can extend the dynamic range of initial template quantification when amplification is monitored with double-stranded DNA specific dyes.
Journal ArticleDOI
Quantitative Analysis of Small-Subunit rRNA Genes in Mixed Microbial Populations via 5′-Nuclease Assays
TL;DR: A newly developed quantitative PCR assay (5′-nuclease assay, also known as TaqMan) was applied to quantify specific small-subunit (SSU) rRNA genes (rDNAs) from uncultivated planktonic prokaryotes in Monterey Bay and accurately measured the proportions of genes in artificial mixtures.
Journal ArticleDOI
Development of an extensive set of 16S rDNA‐targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real‐time PCR
TL;DR: The design and optimization of an extensive set of 16S rDNA‐targeted species‐ and group‐specific primers for more accurate quantification of bacteria from faecal samples with real‐time PCR are reported.
Journal ArticleDOI
Lactobacilli Differentially Modulate Expression of Cytokines and Maturation Surface Markers in Murine Dendritic Cells
TL;DR: The results suggest that different species of Lactobacillus exert very different DC activation patterns and, furthermore, at least one species may be capable of inhibiting activities of other species in the genus.
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