Atg13 and FIP200 act independently of Ulk1 and Ulk2 in autophagy induction
Sebastian Alers,Antje S. Löffler,Florian Paasch,Alexandra M. Dieterle,Hildegard Keppeler,Kirsten Lauber,David G. Campbell,Birgit Fehrenbacher,Martin Schaller,Sebastian Wesselborg,Björn Stork +10 more
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TLDR
In this paper, Atg13 has been shown to be indispensable for autophagy induction in Atg-deficient cells, and the simultaneous knockout of Ulk1 and Ulk2 did not have a similar effect on autophagia induction.Abstract:
Under normal growth conditions the mammalian target of rapamycin complex 1 (mTORC1) negatively regulates the central autophagy regulator complex consisting of Unc-51-like kinases 1/2 (Ulk1/2), focal adhesion kinase family-interacting protein of 200 kDa (FIP200) and Atg13. Upon starvation, mTORC1-mediated repression of this complex is released, which then leads to Ulk1/2 activation. In this scenario, Atg13 has been proposed as an adaptor mediating the interaction between Ulk1/2 and FIP200 and enhancing Ulk1/2 kinase activity. Using Atg13-deficient cells, we demonstrate that Atg13 is indispensable for autophagy induction. We further show that Atg13 function strictly depends on FIP200 binding. In contrast, the simultaneous knockout of Ulk1 and Ulk2 did not have a similar effect on autophagy induction. Accordingly, the Ulk1-dependent phosphorylation sites we identified in Atg13 are expendable for this process. This suggests that Atg13 has an additional function independent of Ulk1/2 and that Atg13 and FIP200 act in concert during autophagy induction.read more
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The emerging roles of ATG1/ATG13 kinase complex in plants.
Qiuling Wang,Suiwen Hou +1 more
TL;DR: In this article , the authors focus on the ATG1/ATG13 kinase complex, summarizing and discussing the current views on the composition, structure, function, and regulation of this complex in plants.
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Emendation of autophagic dysfuction in neurological disorders: a potential therapeutic target.
Shivani R Sainani,Prajakta A. Pansare,Ketki R Rode,Vrushali M Bhalchim,Rohit R Doke,Shivani Desai +5 more
TL;DR: The autophagic process, its impairment in various neurodegenerative and neuropsychiatric disorders along with certain molecules/compounds to fight those impairments are understood.
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Meeting report: Signal transduction meets systems biology
Christine Louis-Dit-Sully,Katharina F. Kubatzky,Jonathan A. Lindquist,Christine Blattner,Ottmar Janssen,Wolfgang W. A. Schamel,Wolfgang W. A. Schamel +6 more
TL;DR: A selection of ongoing projects of SYBILLA are reported on and how they were discussed at this interdisciplinary conference on signal transduction.
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Hyperosmotic Stress Initiates AMPK-Independent Autophagy and AMPK- and Autophagy-Independent Depletion of Thioredoxin 1 and Glyoxalase 2 in HT22 Nerve Cells
TL;DR: Hyperosmotic stress induced AMPK activation, but this was not responsible for its effects on mTOR activity or autophagy induction, as indicated by a decrease in p62 and an increase in LC3 lipidation.
Journal ArticleDOI
Chicken DT40 cell line lacking DJ-1, the gene responsible for familial Parkinson's disease, displays mitochondrial dysfunction.
Eiko N. Minakawa,Hodaka Yamakado,Atsushi Tanaka,Atsushi Tanaka,Kengo Uemura,Shunichi Takeda,Ryosuke Takahashi +6 more
TL;DR: It is suggested that genetically engineered DT40 cells would serve as a relevant model of PD, and help understand the genetic and functional relationship among multiple causative genes, and lead to the development of noninvasive diagnostic tools and drug screening assays using patient-derived lymphocytes.
References
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