Biosensors and Diagnostics for Fungal Detection.
Khalil K. Hussain,Dhara Malavia,Elizabeth M. Johnson,Elizabeth M. Johnson,Jennifer A. Littlechild,C. Peter Winlove,Frank Vollmer,Neil A. R. Gow +7 more
TLDR
This overview provides a critical review of current fungal diagnostics and the development of new biophysical technologies that are being applied for selective new sensitive fungal biosensors to augment traditional diagnostic methodologies.Abstract:
Early detection is critical to the successful treatment of life-threatening infections caused by fungal pathogens, as late diagnosis of systemic infection almost always equates with a poor prognosis. The field of fungal diagnostics has some tests that are relatively simple, rapid to perform and are potentially suitable at the point of care. However, there are also more complex high-technology methodologies that offer new opportunities regarding the scale and precision of fungal diagnosis, but may be more limited in their portability and affordability. Future developments in this field are increasingly incorporating new technologies provided by the use of new format biosensors. This overview provides a critical review of current fungal diagnostics and the development of new biophysical technologies that are being applied for selective new sensitive fungal biosensors to augment traditional diagnostic methodologies.read more
Citations
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AP hotonic Crystal Protein Hydrogel Sensor for Candida albicans
TL;DR: Two-dimensional photonic crystal sensing materials that selectively detect Candida albicans and those microbes devoid of cell-surface mannan are reported, providing a proof-of-concept for utilizing recognition between lectins and microbial cell surface carbohydrates to detect microorganisms in aqueous environments.
Journal ArticleDOI
Specificity Influences in (1→3)-β-d-Glucan-Supported Diagnosis of Invasive Fungal Disease
TL;DR: In this article, a review of the non-IFD related causes of elevated circulating β-glucan (BDG) is presented, including iatrogenic patient contamination through the use of BDG-containing medical devices and parenterally delivered materials as well as translocation of intestinal luminal BDG due to mucosal barrier injury.
Journal ArticleDOI
Architecture of the dynamic fungal cell wall
Neil A. R. Gow,Megan D. Lenardon +1 more
TL;DR: The spatial organization and dynamic regulation of the wall in response to prevailing growth conditions enable fungi to thrive within changing, diverse and often hostile environments.
Journal ArticleDOI
Identifying Mucormycosis Severity in Indian COVID-19 Patients: A Nano-Based Diagnosis and the Necessity for Critical Therapeutic Intervention
Syed Mohammed Basheeruddin Asdaq,Arya Rajan,Aswin Damodaran,Shivali R Kamath,Krishnanjana S. Nair,Subin Mary Zachariah,Ram Kumar Sahu,Ram Kumar Sahu,Santosh Fattepur,Nagaraja Sreeharsha,Anroop B. Nair,Shery Jacob,Hussain A Albahrani,Eman H. Alkhaldi,Yahya Mohzari,Ahmed A. Alrashed,Mohd. Imran +16 more
TL;DR: In this paper, the authors analyzed the existing statistical data to address the severity of prevalence and further noted the nano-based diagnostic parameters, clinical presentations, its connection with other conditions like diabetes, hypertension, and GI disorders, and the importance of anti-fungal therapy in treating the same.
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Validation of New ELISA Technique for Detection of Aflatoxin B1 Contamination in Food Products versus HPLC and VICAM.
Elsayed E. Hafez,Nourhan M. Abd El-Aziz,Amira M. G. Darwish,Mohamed G. Shehata,Amira A. Ibrahim,Asmaa M. Elframawy,Ahmed Noah Badr +6 more
TL;DR: In this paper, a quantitative recombinant AflR gene antiserum ELISA technique for aflatoxin B1 detection in contaminated food products was developed, which was used to induce the production of IgG antibodies in rabbits.
References
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Journal ArticleDOI
Hidden Killers: Human Fungal Infections
Gordon D. Brown,David W. Denning,Neil A. R. Gow,Stuart M. Levitz,Mihai G. Netea,Theodore C. White +5 more
TL;DR: The importance of fungi as human pathogens is highlighted and the challenges the authors face in combating the devastating invasive infections caused by these microorganisms are discussed, in particular in immunocompromised individuals.
Journal ArticleDOI
Sensitive optical biosensors for unlabeled targets: a review.
TL;DR: This article reviews the recent progress in optical biosensors that use the label-free detection protocol, in which biomolecules are unlabeled or unmodified, and are detected in their natural forms, and focuses on the optical biosENSors that utilize the refractive index change as the sensing transduction signal.
Journal ArticleDOI
Delaying the Empiric Treatment of Candida Bloodstream Infection until Positive Blood Culture Results Are Obtained: a Potential Risk Factor for Hospital Mortality
TL;DR: Delayed treatment of Candida bloodstream infections could be minimized by the development of more rapid diagnostic techniques for the identification of CandIDA bloodstream infections and increased use of empiric antifungal treatment in selected patients at high risk for fungal bloodstream infection could also reduce delays in treatment.
Journal ArticleDOI
Revision and Update of the Consensus Definitions of Invasive Fungal Disease From the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium
J. Peter Donnelly,Sharon C.-A. Chen,Carol A. Kauffman,William J. Steinbach,John W. Baddley,Paul E. Verweij,Cornelius J. Clancy,John R. Wingard,Shawn R. Lockhart,Andreas H. Groll,Tania C. Sorrell,Matteo Bassetti,Hamdi Akan,Barbara D. Alexander,David R. Andes,Elie Azoulay,Ralf Bialek,Robert W. Bradsher,Stéphane Bretagne,Thierry Calandra,Angela M. Caliendo,Elio Castagnola,Mario Cruciani,Manuel Cuenca-Estrella,Catherine F. Decker,Sujal R. Desai,Brian T. Fisher,Thomas S. Harrison,Claus Peter Heussel,Henrik Jeldtoft Jensen,Christopher C. Kibbler,Dimitrios P. Kontoyiannis,Bart Jan Kullberg,Katrien Lagrou,Frédéric Lamoth,Thomas Lehrnbecher,J. Loeffler,Olivier Lortholary,Johan Maertens,O. Marchetti,Kieren A. Marr,Henry Masur,Jacques F. Meis,C. Orla Morrisey,Marcio Nucci,Luis Ostrosky-Zeichner,Livio Pagano,Thomas F. Patterson,John R. Perfect,Zdenek Racil,Emmanuel Roilides,Marcus Ruhnke,Cornelia Schaefer Prokop,Shmuel Shoham,Monica A. Slavin,David A. Stevens,George Richard Thompson,Jose A. Vazquez,Claudio Viscoli,Thomas J. Walsh,Adilia Warris,L. Joseph Wheat,P. Lewis White,Theoklis E. Zaoutis,Peter G. Pappas +64 more
TL;DR: These updated definitions of IFDs should prove applicable in clinical, diagnostic, and epidemiologic research of a broader range of patients at high-risk.
Journal ArticleDOI
An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
Nur Royhaila Mohamad,Nur Haziqah Che Marzuki,Nor Aziah Buang,Fahrul Huyop,Roswanira Abdul Wahab +4 more
TL;DR: Characterization technologies at the nanoscale level to study enzymes immobilized on surfaces are crucial to obtain valuable qualitative and quantitative information, including morphological visualization of the immobilized enzymes, to assess efficacy of an immobilization technique and development of future enzyme immobilization strategies.