Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
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The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-15 and is currently open access. It has received 1147 citations till now. The article focuses on the topics: CYP8B1 & Cholesterol 7 alpha-hydroxylase.read more
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Analysis of the importance of arginine 102 in neutral endopeptidase (enkephalinase) catalysis.
TL;DR: Results suggest that the contribution of arginine 102 to substrate binding is dependent upon the strength of other subsite interactions, and Examination of dipeptides as inhibitors indicates that the nature and orientation of the P'2 residue is important in determining thestrength of the interaction of arkinine 102 with its substrates.
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CYP4Fs Expression in Rat Brain Correlates with Changes in LTB4 Levels after Traumatic Brain Injury
Ying Wang,Jing Zhao,Auinash Kalsotra,Cheri M. Turman,Raymond J. Grill,Pramod K. Dash,Henry W. Strobel +6 more
TL;DR: It is shown that traumatic brain injury triggers inflammation and elicits changes in mRNA expression of CYP4Fs in the frontal and occipital lobes and the hippocampus, which inversely correlate with levels of leukotriene B4 levels in the brain following injury at the same time points.
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Does the overexpression of pro-insulin-like growth factor-II in transfected human embryonic kidney fibroblasts increase the secretion of lysosomal enzymes?
Andreas Hoeflich,E. Wolf,Thomas Braulke,Gabriele Koepf,Ulrike Kessler,Gottfried Brem,Wolfgang Rascher,Werner F. Blum,Wieland Kiess +8 more
TL;DR: The results indicate that the overexpression of pro-IGF-II doubles the secretion and/or reduces the re-uptake of beta-hexosaminidase and cathepsin D to approximately 20% of the total synthesized enzymes in human embryonal kidney fibroblasts compared to control cells.
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Two new mutations in the sterol 27-hydroxylase gene in two families lead to cerebrotendinous xanthomatosis
Aad Verrips,G.C.H. Steenbergen-Spanjers,J.A.F.M. Luyten,L.P.W.J. van den Heuvel,Antoine Keyser,Fons J. M. Gabreëls,Ron A. Wevers +6 more
TL;DR: In this article, the sterol 27-hydroxylase gene in two patients with cerebrotendinous xanthomatosis (CTX) was found to have two new mutations.
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Variable Nuclear Cytoplasmic Distribution of the 11.5-kDa Zinc-binding Protein (Parathymosin-α) and Identification of a Bipartite Nuclear Localization Signal
Hans-Ingo Trompeter,Gabriele Blankenburg,Britta Brügger,Jutta Menne,Andreas Schiermeyer,Matthias Scholz,Hans-Dieter Söling +6 more
TL;DR: It is shown here that wild-type ZnBP overexpressed in COS cells accumulates exclusively in the nucleus but that Zn BP with a mutated or deleted PKRQKT motif appears both inThe nucleus and in the cytoplasm.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
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A receptor-mediated pathway for cholesterol homeostasis.
TL;DR: The approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH, which led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis.