Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
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The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-15 and is currently open access. It has received 1147 citations till now. The article focuses on the topics: CYP8B1 & Cholesterol 7 alpha-hydroxylase.read more
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Book ChapterDOI
Metabolism and Catabolism of Vitamin D, Its Metabolites, and Clinically Relevant Analogs
TL;DR: This review highlights the recent finding of extra-renal sites of CYP27B1 expression and the physiological implications of this discovery, as well as attempting to predict future directions in the field, focussing on determination of CYp structure, the knowledge gained from mouse CYP knockouts and future vitamin D drug design.
Journal ArticleDOI
Repression of apoC-III gene expression by TNFα involves C/EBPδ/NF-IL6β via an IL-1 independent pathway
TL;DR: Using transient transfection, it is found that apoC‐III gene expression is controlled at the transcriptional level and activation of C/EBPδ by TNFα is not related to the IL‐1‐signalling pathway.
Journal ArticleDOI
Expression of key enzymes in bile acid biosynthesis during development: CYP7B1-mediated activities show tissue-specific differences.
TL;DR: The results indicate that the expression of CYP27A1 and 3beta-hydroxy-Delta(5)-C(27)-steroid dehydrogenase are similar in livers of newborn and 6-month-old pigs whereas the levels of CYB7A1 increase.
Journal ArticleDOI
Microsomal 25-hydroxylation of vitamin D2 and vitamin D3 in pig liver
TL;DR: Results show that the 25-hydroxylation of vitamin D2 and D3 is catalysed by the same microsomal cytochrome P-450 in pig liver microsomes, with the same apparent M(r), substrate specificity and N-terminal amino acid sequence as the previously purified vitamin D3 25-Hydroxylase from pig livermicrosomes.
Journal ArticleDOI
In vitro correction of ARSA deficiency in human skin fibroblasts from metachromatic leukodystrophy patients after treatment with microencapsulated recombinant cells.
Valeska Lizzi Lagranha,Guilherme Baldo,Talita Giacomet de Carvalho,Maira Graeff Burin,Maria Luiza Saraiva-Pereira,Ursula da Silveira Matte,Roberto Giugliani +6 more
TL;DR: Encapsulated rBHK clones show potential as a new therapeutic strategy for the treatment of MLD, reaching normal enzyme levels in human MLD fibroblasts.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
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A receptor-mediated pathway for cholesterol homeostasis.
TL;DR: The approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH, which led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis.