Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
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The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-15 and is currently open access. It has received 1147 citations till now. The article focuses on the topics: CYP8B1 & Cholesterol 7 alpha-hydroxylase.read more
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Journal ArticleDOI
Exon 11 of the rat cholesterol esterase gene encodes domains important for intracellular processing and bile salt-modulated activity of the protein
TL;DR: The cholesterol esterases with reducing numbers of the proline-rich repeating units were also active in hydrolyzing p-nitrophenyl butyrate and cholesteryl oleate and were more active than the native enzyme in substrate hydrolysis at low bile salt concentrations.
Journal ArticleDOI
Estrogen receptor α attenuates transforming growth factor-β signaling in breast cancer cells independent from agonistic and antagonistic ligands
TL;DR: A permanent repression of PAI-1 by ERα is indicated and a ligand-independent crosstalk between ERα and TGF-β signaling in breast cancer cells is suggested, which is completely independent of receptor stimulation by β-estradiol (E2) or inhibition by the pure antagonist ICI 182.780.
Book ChapterDOI
Vitamin D Biology
René St-Arnaud,Marie B. Demay +1 more
TL;DR: This chapter reviews several aspects of vitamin D biology, including the transcriptional activity of the VDR, which is modulated by posttranslational modifications as well as by association with nuclear receptor coactivators.
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Sterol Regulatory Element-binding Proteins Activate Insulin Gene Promoter Directly and Indirectly through Synergy with BETA2/E47
Michiyo Amemiya-Kudo,Junko Oka,Tomohiro Ide,Takashi Matsuzaka,Hirohito Sone,Tomohiro Yoshikawa,Naoya Yahagi,Shun Ishibashi,Jun-ichi Osuga,Nobuhiro Yamada,Toshio Murase,Hitoshi Shimano +11 more
TL;DR: It is demonstrated that the insulin promoter is a novel target for SREBPs established as lipid-synthetic transcription factors, and cryptic SREBP-1c action might play a compensatory role in insulin expression in diabetes with β cell lipotoxicity.
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Different 5'-flanking regions of the inhibin-alpha gene target transgenes to the gonad and adrenal in an age-dependent manner in transgenic mice.
TL;DR: The 2.5-kb inhibin-alpha 5'-proximal DNA sequence directs transgene expression in mature ovarian follicles and testicular Sertoli cells and is useful for future analysis of gonadal and adrenal cell functions.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
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A receptor-mediated pathway for cholesterol homeostasis.
TL;DR: The approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH, which led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis.