Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
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The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-15 and is currently open access. It has received 1147 citations till now. The article focuses on the topics: CYP8B1 & Cholesterol 7 alpha-hydroxylase.read more
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Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination.
Olivier Staub,Ivan Gautschi,Toru Ishikawa,Kristin Breitschopf,Aaron Ciechanover,Laurent Schild,Daniela Rotin +6 more
TL;DR: It is shown that ENaC is a short‐lived protein that is ubiquitinated in vivo on the α and γ (but not β) subunits, and a paradigm for ubiquitination‐mediated regulation of ion channels is proposed.
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GS domain mutations that constitutively activate T beta R-I, the downstream signaling component in the TGF-beta receptor complex.
TL;DR: The identification of constitutively active T beta R‐I forms confirms the hypothesis that this kinase acts as a down‐stream signaling component in the TGF‐beta receptor complex, and its activation by T Beta R‐II or by mutation is necessary and sufficient for propagation of anti‐proliferative and transcriptional responses.
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Molecular cloning and expression of the gene for a human D1 dopamine receptor.
Alien Dearry,Jay A. Gingrich,Pierre Falardeau,Robert T. Fremeau,Michael D. Bates,Marc G. Caron,Marc G. Caron +6 more
TL;DR: Transient or stable expression of the cloned gene in host cells established specific ligand binding and functional activity characteristic of a D1 dopamine receptor coupled to stimulation of adenylyl cyclase.
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Identification of human activin and TGFβ type I receptors that form heteromeric kinase complexes with type II receptors
Liliana Attisano,Juan M. Cárcamo,Francesc Ventura,Frances M.B. Weis,Joan Massagué,Jeffrey L. Wrana +5 more
TL;DR: The results indicate that type I receptors are transmembrane protein kinases that associate with type II receptors to generate diverse heteromeric serine/threonine kinase complexes of different signaling capacities.
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The syntaxin family of vesicular transport receptors
Mark K. Bennett,JoséE. Garcia-Arrarás,Lisa A. Elferink,Karen E. Peterson,Anne M. Fleming,Christopher D. Hazuka,Richard H. Scheller +6 more
TL;DR: Results indicate that the syntaxins are a family of receptors for intracellular transport vesicles and that each target membrane may be identified by a specific member of the syntaxin family.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
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A receptor-mediated pathway for cholesterol homeostasis.
TL;DR: The approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH, which led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis.