Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
Reads0
Chats0
TLDR
The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-15 and is currently open access. It has received 1147 citations till now. The article focuses on the topics: CYP8B1 & Cholesterol 7 alpha-hydroxylase.read more
Citations
More filters
Journal ArticleDOI
Characterization of two udp glucuronosyltransferases that are predominantly expressed in human colon
TL;DR: Analysis of human colon RNA samples revealed the presence of two UDP glucuronosyltransferase forms that could not be detected in human liver, and showed differential activity towards several phenolic substrates, suggesting that UGT 1A8 and UGT1A10 may play an important role in the metabolism of dietary xenobiotics.
Journal ArticleDOI
The Gfi-1 protooncoprotein represses Bax expression and inhibits T-cell death
TL;DR: It is shown that overexpression of Gfi-1 also inhibits cell death induced by cultivation of IL-2-dependent T-cell lines in IL- 2-deficient media and may inhibit apoptosis by means of its repression of multiple proapoptotic regulators.
Journal ArticleDOI
Cloning and sequence analysis of a cDNA encoding human non-selective type of endothelin receptor.
Makoto Nakamuta,Ryoichi Takayanagi,Yoshiyuki Sakai,Shigeru Sakamoto,Hiromi Hagiwara,Takeshi Mizuno,Yasuyoshi Saito,Shigehisa Hirose,Mikio Yamamoto,Hajime Nawata +9 more
TL;DR: A cDNA encoding non-selective type (ETB) of endothelin receptor was isolated from a human liver cDNA library and contained a relatively long and proline-rich extracellular N-terminal region in addition to a significant similarity with the G protein-coupled receptor super-family with seven transmembrane segments.
Journal ArticleDOI
Tyr-179 and Lys-183 are essential for enzymatic activity of 11β-hydroxysteroid dehydrogenase
Jihad S. Obeid,Perrin C. White +1 more
TL;DR: Tyr-179 and Lys-183 are likely to be functionally important residues in 11 beta-hydroxysteroid dehydrogenase, as these amino acids are absolutely conserved in all members of the "short chain dehydrogen enzyme" family.
Journal ArticleDOI
c-Myb and core-binding factor/PEBP2 display functional synergy but bind independently to adjacent sites in the T-cell receptor delta enhancer.
TL;DR: It is proposed that spatially precise synergy between CBF/PEBP2 and c-Myb may result from the ability of the two factors to form a composite surface that makes unique and stereospecific contacts with one or more additional components of the transcriptional machinery.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
Journal ArticleDOI
A receptor-mediated pathway for cholesterol homeostasis.
TL;DR: The approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH, which led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis.