Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
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The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-15 and is currently open access. It has received 1147 citations till now. The article focuses on the topics: CYP8B1 & Cholesterol 7 alpha-hydroxylase.read more
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Characterisation of high-affinity and low-affinity receptors for ciliary neurotrophic factor
TL;DR: Antisense experiments confirmed that CNTFR alpha is necessary for high affinity binding of 125I-CNTF and therefore a necessary subunit of the high-affinity receptor.
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Cerebrotendinous xanthomatosis with predominant parkinsonian syndrome: further confirmation of the clinical heterogeneity.
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Anabolic-androgenic steroid interaction with rat androgen receptor in vivo and in vitro : A comparative study
Boris Feldkoren,Stefan Andersson +1 more
TL;DR: Stanozolol and methanedienone are low affinity ligands of the rat recombinant AR as revealed by a ligand binding assay in vitro, however, based on a cell-based AR-dependent transactivation assay, they are potent activators of the AR.
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Vps11 and Vps18 of Vps-C membrane traffic complexes are E3 ubiquitin ligases and fine-tune signalling.
Gregory Segala,Marcela A. Bennesch,Nastaran Mohammadi Ghahhari,Deo Prakash Pandey,Deo Prakash Pandey,Pablo Christian Echeverria,François Karch,Robert K. Maeda,Didier Picard +8 more
TL;DR: It is shown that Vps11 and Vps18 (Vps11/18), two common subunits of the HOPS/CORVET complexes, are E3 ubiquitin ligases that are involved in the regulation of ERα signalling.
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Determination of amino acid residues that are accessible from the ligand binding crevice in the seventh transmembrane-spanning region of the human A(1) adenosine receptor.
Eric S. Dawson,Jack N. Wells +1 more
TL;DR: The substituted-cysteine accessibility method was applied to transmembrane span seven of the human A(1) adenosine receptor to reveal a subset of amino acids that are exposed to the ligand-binding crevice and suggests that H278 may not be required for binding of antagonist ligands.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
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A receptor-mediated pathway for cholesterol homeostasis.
TL;DR: The approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH, which led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis.