Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
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The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-15 and is currently open access. It has received 1147 citations till now. The article focuses on the topics: CYP8B1 & Cholesterol 7 alpha-hydroxylase.read more
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Sp2 Localizes to Subnuclear Foci Associated with the Nuclear Matrix
K. Scott Moorefield,Haifeng Yin,Teresa D. Nichols,Christopher Cathcart,Steven O. Simmons,Jonathan M. Horowitz +5 more
TL;DR: It is concluded that Sp2 preferentially associates with the nuclear matrix and speculate that this subcellular localization plays an important role in the regulation of Sp2 function.
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Isolation and characterization of a novel transcription factor that binds to and activates insulin control element-mediated expression.
TL;DR: Ind isolation and characterization of a cDNA for a protein present in alpha and beta cells, termed INSAF for insulin activator factor, which binds to and activates ICE-mediated expression are described, consistent with the proposal that InSAF is either part of the ICE activator or is antigenically related to the specific activator required for insulin gene transcription.
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Replacement of interleukin-2 (IL-2)-generated mitogenic signals by a mink cell focus-forming (MCF) or xenotropic virus-induced IL-9-dependent autocrine loop: implications for MCF virus-induced leukemogenesis.
TL;DR: Evidence is presented that the vast majority of IL-2-independent lines established from polytropic or xenotropic virus-infected 4437A cells carry provirus insertions in the 3' untranslated region of the IL-9 receptor gene (Gfi-2 [for growth factor independence-2]/IL-9R).
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Interactions of the Amino Acid Residue at Position 31 of the c-Ha-Ras Protein with Raf-1 and RalGDS
Mikako Shirouzu,Kenji Morinaka,Shinya Koyama,Chang-Deng Hu,Naoko Hori-Tamura,Tomoyo Okada,Ken-ichi Kariya,Tohru Kataoka,Akira Kikuchi,Shigeyuki Yokoyama +9 more
TL;DR: Results clearly show that the sharp contrast between the characteristics of Ras and Rap1A, with respect to the interactions with Raf-1 and RalGDS, depends on their residues at position 31.
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Synergistic action of R-Ras and IGF-1 on Bcl-xL expression and caspase-3 inhibition in BaF3 cells: R-Ras and IGF-1 control distinct anti-apoptotic kinase pathways
TL;DR: R‐Ras and insulin‐like growth factor‐1 (IGF‐1) synergistically inhibit apoptosis of BaF3 cells upon interleukin‐3 deprivation and the mechanism of this synergistic inhibition was characterized.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
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A receptor-mediated pathway for cholesterol homeostasis.
TL;DR: The approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH, which led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis.