Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
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The structure of the sterol 26-hydroxylase cDNA reveals it to be a mitochondrial cytochrome P-450, and blotting experiments revealed that the mRNA for this enzyme is expressed in many tissues and that it is encoded by a low copy number gene in the rabbit genome.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-15 and is currently open access. It has received 1147 citations till now. The article focuses on the topics: CYP8B1 & Cholesterol 7 alpha-hydroxylase.read more
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Cloning of a novel receptor expressed in rat prostate and ovary.
TL;DR: It is concluded that clone 29 cDNA encodes a novel rat ER, which is suggested be named rat ERbeta to distinguish it from the previously cloned ER (ERalpha) from rat uterus.
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JNK1: A protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain
Benoit Dérijard,Benoit Dérijard,Masahiko Hibi,I-Huan Wu,I-Huan Wu,Tamera Barrett,Bing Su,Tiliang Deng,Michael Karin,Roger J. Davis,Roger J. Davis +10 more
TL;DR: JNK1 is a component of a novel signal transduction pathway that is activated by oncoproteins and UV irradiation and its properties indicate that JNK1 activation may play an important role in tumor promotion.
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Mechanism of activation of protein kinase B by insulin and IGF-1.
Dario R. Alessi,Mirjana Andjelkovic,Barry Caudwell,Peter Cron,Nick Morrice,Philip Cohen,Brian A. Hemmings +6 more
TL;DR: In this paper, the activation of PKBalpha was accompanied by its phosphorylation at Thr308 and Ser473 and, like activation, likeactivation was prevented by the phosphatidylinositol 3-kinase inhibitor wortmannin.
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Pro-inflammatory Cytokines and Environmental Stress Cause p38 Mitogen-activated Protein Kinase Activation by Dual Phosphorylation on Tyrosine and Threonine (∗)
Joel Raingeaud,Shashi Gupta,Jeffrey Scott Rogers,Martin Dickens,Jiahuai Han,Richard J. Ulevitch,Roger J. Davis,Roger J. Davis +7 more
TL;DR: It is demonstrated that p38, like JNK, is activated by treatment of cells with pro-inflammatory cytokines and environmental stress and the mechanism of p38 activation is mediated by dual phosphorylation on Thr-180 and Tyr-182.
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cPLA2 is phosphorylated and activated by MAP kinase.
TL;DR: Treatment of cells with agents that stimulate the release of arachidonic acid causes increased serine phosphorylation and activation of cytosolic phospholipase A2 (cPLA2).
References
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Sex differences in cytochrome P-450-dependent 25-hydroxylation of C27-steroids and vitamin D3 in rat liver microsomes.
S Andersson,H Jörnvall +1 more
TL;DR: Andersson et al. as discussed by the authors presented a monoclonal antibody, designated MAb-25-6, which was able to bind to cytochrome P-450(25) and immunoprecipitate the activity for 25-hydroxylation of 5 beta-cholestane-3 alpha,7 alpha,12 alpha-triol and vitamin D3.
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25-Hydroxylation of vitamin D3 by a cytochrome P-450 from rabbit liver mitochondria.
H Dahlbäck,Kjell Wikvall +1 more
TL;DR: The results raise the possibility that the 25-hydroxylation of vitamin D3 and the 26-hydrogenation of C27 steroids are catalysed by the same species of cytochrome P-450 in liver mitochondria.
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Evidence that the 5'-untranslated leader of mRNA affects the requirement for wheat germ initiation factors 4A, 4F, and 4G.
TL;DR: Determination of the amounts of the initiation factors required for translation in the fractionated system showed that AMV RNA 4 required 2-4-fold lower amounts than did B alpha A mRNA, and findings indicated that the mRNAs containing the Balpha A leader sequence required higher amounts of one or more of the Initiation factors for efficient translation.
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Mechanism of androstenedione formation from testosterone and epitestosterone catalyzed by purified cytochrome P-450b.
Alexander W. Wood,David C. Swinney,Paul E. Thomas,Dene E. Ryan,P F Hall,Wayne Levin,W A Garland +6 more
TL;DR: The results indicate that androstenedione formation from epitestosterone proceeds exclusively through the gem-diol pathway, while androstene formation from testosterone may proceed through a combination of gem- diol and dual hydrogen abstraction pathways.
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Regulation of low density lipoprotein receptor gene expression in human lymphocytes.
TL;DR: Data show that the initial upregulation of LDL receptor gene expression is independent of protein synthesis and not suppressed by either LDL or oxygenated sterols, and in contrast, the continued transcription necessary for the maintenance of steady-state levels of LDL receptors requires synthesis of new protein and is regulated by LDL and oxygenate sterols.