Journal ArticleDOI
Development of a Universal Fluorescent Probe for Gram-Positive Bacteria.
Haw-Young Kwon,Xiao Liu,Eun Gyeong Choi,Jung Yeol Lee,So Young Choi,Junyoung Kim,Lu Wang,Sungjin Park,Beomsue Kim,Yong-An Lee,Jong-Jin Kim,Nam-Young Kang,Young-Tae Chang +12 more
TLDR
A fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram-positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria.Abstract:
The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram-positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect a Gram-positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented research tool for the study of dynamic bacterial communities and for clinical application.read more
Citations
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Journal ArticleDOI
Evaluation of Structure-Function Relationships of Aggregation-Induced Emission Luminogens for Simultaneous Dual Applications of Specific Discrimination and Efficient Photodynamic Killing of Gram-Positive Bacteria.
Miaomiao Kang,Miaomiao Kang,Chengcheng Zhou,Shuangmei Wu,Bingran Yu,Zhijun Zhang,Zhijun Zhang,Nan Song,Nan Song,Michelle M. S. Lee,Wenhan Xu,Fu-Jian Xu,Dong Wang,Lei Wang,Ben Zhong Tang +14 more
TL;DR: Evaluation of structure-function relationships reveals that AIE luminogens (AIEgens) having intrinsic positive charge and proper ClogP value are able to stain Gram-positive bacteria.
Journal ArticleDOI
Recent progress in fluorescent probes for bacteria.
TL;DR: In this article, the authors summarize the recent progress in bacterial detection using fluorescence and generalize the mechanisms commonly used to design organic fluorescent probes for detecting and imaging bacteria, and discuss a perspective regarding fluorescent probe for bacterial detection.
Journal ArticleDOI
Maltotriose-based probes for fluorescence and photoacoustic imaging of bacterial infections
TL;DR: A fluorescent derivative of maltotriose is developed, which is shown to be taken up in a variety of gram-positive and gram-negative bacterial strains in vitro, and an ideal scaffold for infection imaging agents encompassing better pharmacokinetic properties and in vivo stability than other maltodextrins.
Journal ArticleDOI
A boronic acid-functionalized phthalocyanine with an aggregation-enhanced photodynamic effect for combating antibiotic-resistant bacteria
TL;DR: A boronic acid functionalized phthalocyanine displays aggregation-enhanced reactive oxygen species (ROS) generation and excellent photodynamic antibacterial activity.
Journal ArticleDOI
One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens.
Chengcheng Zhou,Meijuan Jiang,Meijuan Jiang,Jian Du,Haotian Bai,Guo-Gang Shan,Ryan T. K. Kwok,Joe H. C. Chau,Jun Zhang,Jacky Wing Yip Lam,Peng Huang,Ben Zhong Tang,Ben Zhong Tang +12 more
TL;DR: A microenvironment-sensitive aggregation-induced emission luminogen (AIEgen), namely IQ–Cm, is developed for achieving fast discrimination of Gram-negative bacteria, Gram-positive bacteria and fungi by the naked-eye for rapid pathogen detection and point-of-care diagnosis.
References
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Journal ArticleDOI
Symbiotic Bacteria Direct Expression of an Intestinal Bactericidal Lectin
TL;DR: It is proposed that RegIIIγ and its human counterpart, HIP/PAP, are directly antimicrobial proteins that bind their bacterial targets via interactions with peptidoglycan carbohydrate, and that they reveal intestinal strategies for maintaining symbiotic host-microbial relationships.
Journal ArticleDOI
Interplay between NS3 protease and human La protein regulates translation-replication switch of Hepatitis C virus
Upasana Ray,Saumitra Das +1 more
TL;DR: It is demonstrated that the NS3 protease (NS3pro) domain alone can specifically bind to HCV-IRES RNA, predominantly in the SLIV region, and that this binding reduces translation in favor of RNA replication.
Journal ArticleDOI
Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge.
TL;DR: In this article, a genus-specific 16S rRNA-targeted oligonucleotide probe was developed to investigate the role of Acinetobacter spp. in anaerobic and aerobic compartments of a sewage treatment plant with enhanced biological phosphate removal.
Journal ArticleDOI
Heterotrophic plate count bacteria—what is their significance in drinking water?
TL;DR: The relevant literature on HPC bacteria in drinking water, the lack of clinical evidence that elevated populations or specific genera within the HPC flora pose an increased health risk to any segment of the population, and the appropriate uses of HPC data as a tool to monitor drinking water quality changes following treatment are reviewed are reviewed.
Journal ArticleDOI
Ryder Glacier in northwest Greenland is shielded from warm Atlantic water by a bathymetric sill
Martin Jakobsson,Larry A. Mayer,Johan Nilsson,Christian Stranne,Brian R. Calder,Matt O'Regan,John W. Farrell,Thomas M. Cronin,Volker Brüchert,Julek Chawarski,Björn Eriksson,Jonas Fredriksson,Laura Gemery,Anna Glueder,Felicity A. Holmes,Kevin Jerram,Nina Kirchner,Alan C. Mix,Julia Muchowski,Abhay Prakash,Brendan T Reilly,Brett F. Thornton,Adam Ulfsbo,Elizabeth Weidner,Elizabeth Weidner,Henning Åkesson,Tamara Handl,Emelie Ståhl,Lee-Gray Boze,Sam Reed,Gabriel West,June Padman +31 more
TL;DR: In this paper, a bathymetric sill in Sherard Osborn Fjord, northwest Greenland shields Ryder Glacier from melting by warm Atlantic water found at the bottom of the fjord.