Differential Behaviors of Atrial Versus Ventricular Fibroblasts A Potential Role for Platelet-Derived Growth Factor in Atrial-Ventricular Remodeling Differences
TLDR
Atrial fibroblasts behave differently than ventricular fibro Blasts over a range of in vitro and in vivo paradigms, with atrial Fibroblast showing enhanced reactivity that may explain greater atrial fibrotic responses.Abstract:
Background— In various heart disease paradigms, atria show stronger fibrotic responses than ventricles. The possibility that atrial and ventricular fibroblasts respond differentially to pathological stimuli has not been examined. Methods and Results— We compared various morphological, secretory, and proliferative response indexes of canine atrial versus ventricular fibroblasts. Cultured atrial fibroblasts showed faster cell surface area increases, distinct morphology at confluence, and greater α-smooth muscle actin expression than ventricular fibroblasts. Atrial fibroblast proliferation ([3H]thymidine incorporation) responses were consistently greater for a range of growth factors, including fetal bovine serum, platelet-derived growth factor (PDGF), basic fibroblast growth factor, angiotensin II, endothelin-1, and transforming growth factor-β1. Normal atrial tissue showed larger myofibroblast density compared with ventricular tissue, and the difference was exaggerated by congestive heart failure. Congesti...read more
Citations
More filters
Journal ArticleDOI
Obesity and atrial fibrillation.
Hany S. Abed,Gary A. Wittert +1 more
TL;DR: The evidence for an obesity‐related atrial electromechanical dysfunction, the mechanisms behind this and its impact on AF therapeutic outcomes are reviewed and proposed management approaches and avenues for further investigations are illustrated.
Journal ArticleDOI
The Origin and Arrhythmogenic Potential of Fibroblasts in Cardiac Disease
TL;DR: The available evidence supporting the contribution of intracardiac and extracardiac sources to the fibroblast and myofibroblast populations in diseased hearts is discussed.
Journal ArticleDOI
The clinical significance of the atrial subendocardial smooth muscle layer and cardiac myofibroblasts in human atrial tissue with valvular atrial fibrillation.
TL;DR: SSM layer was closely related to the existence of AF, degrees of atrial remodeling, and fibrosis in patients who underwent open heart surgery and it was found that MFB does exist in SSM layer of human atrial tissue co-localized with PAS-positive cells.
Journal ArticleDOI
Tetrandrine reverses human cardiac myofibroblast activation and myocardial fibrosis
Guoqi Teng,Daniyil A. Svystonyuk,Holly E.M. Mewhort,Jeannine D. Turnbull,Darrell D. Belke,Henry J. Duff,Paul W.M. Fedak +6 more
TL;DR: TTD reverses human cardiac myofibroblast activation and myocardial fibrosis, independent of calcium channel blockade, and inhibited collagen gel contraction in the presence of T and L-type calcium channel blockers, and the intracellular calcium chelator BAPTA-AM, suggesting that the observed effects are not mediated by calcium homeostasis.
Journal ArticleDOI
Angiotensin II signaling up-regulates the immediate early transcription factor ATF3 in the left but not the right atrium.
TL;DR: The data strongly place ATF3 as a unique nuclear protein target in response to angiotensin II stimulation in the atria, and may add to the understanding of the signaling pathways involved in cardiac response to neuro-hormonal stimulation, and in particular to theUnderstanding of left atrial-generated pathology such as atrial fibrillation.
References
More filters
Journal ArticleDOI
Endothelial-to-mesenchymal transition contributes to cardiac fibrosis
Elisabeth M. Zeisberg,Oleg Tarnavski,Michael Zeisberg,Adam L. Dorfman,Julie R. McMullen,Erika Gustafsson,Anil Chandraker,Xueli Yuan,William T. Pu,Anita B. Roberts,Eric G. Neilson,Mohamed H. Sayegh,Seigo Izumo,Raghu Kalluri +13 more
TL;DR: It is shown that cardiac fibrosis is associated with the emergence of fibroblasts originating from endothelial cells, suggesting an endothelial-mesenchymal transition (EndMT) similar to events that occur during formation of the atrioventricular cushion in the embryonic heart.
Journal ArticleDOI
Promotion of atrial fibrillation by heart failure in dogs: atrial remodeling of a different sort.
TL;DR: Experimental CHF strongly promotes the induction of sustained AF by causing interstitial fibrosis that interferes with local conduction, with important potential implications for understanding, treating, and preventing AF related to CHF.
Journal ArticleDOI
Diversity, topographic differentiation, and positional memory in human fibroblasts
Howard Y. Chang,Jen-Tsan Chi,Sandrine Dudoit,Chanda Bondre,Matt van de Rijn,David Botstein,Patrick O. Brown +6 more
TL;DR: Cultured fetal and adult human fibroblasts maintained key features of HOX gene expression patterns established during embryogenesis, suggesting that HOX genes may direct topographic differentiation and underlie the detailed positional memory in fibro Blasts.
Journal ArticleDOI
PDGF-A signaling is a critical event in lung alveolar myofibroblast development and alveogenesis
Hans Boström,Karen Willetts,Karen Willetts,Milos Pekny,Per Levéen,Per Lindahl,Håkan Hedstrand,Marcela Pekna,Mats Hellström,Samuel Gebre-Medhin,Martin Schalling,Mia Nilsson,Siri Kurland,Jan Törnell,John K. Heath,Christer Betsholtz +15 more
TL;DR: The two PDGF null phenotypes reveal analogous morphogenetic functions for myofibroblast-type cells in lung and kidney organogenesis, and show that PDGF-B is required in the ontogeny of kidney mesangial cells.
Journal ArticleDOI
TGF-β induces bimodal proliferation of connective tissue cells via complex control of an autocrine PDGF loop
TL;DR: TGF-beta induces proliferation of connective tissue cells at low concentrations by stimulating autocrine PDGF-AA secretion, which at higher concentrations of TGF- beta, is decreased by down-regulation of PDGF receptor alpha subunits and perhaps by direct growth inhibition.
Related Papers (5)
Atrial fibrosis: mechanisms and clinical relevance in atrial fibrillation.
Brett Burstein,Stanley Nattel +1 more
Promotion of atrial fibrillation by heart failure in dogs: atrial remodeling of a different sort.
MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts
Thomas Thum,Carina Gross,Jan Fiedler,Thomas Fischer,Stephan Kissler,Markus Bussen,Paolo Galuppo,Steffen Just,Wolfgang Rottbauer,Stefan Frantz,Mirco Castoldi,Jürgen Soutschek,Victor Koteliansky,Andreas Rosenwald,M. Albert Basson,Jonathan D. Licht,John T. R. Pena,Sara H. Rouhanifard,Martina U. Muckenthaler,Thomas Tuschl,Gail R. Martin,Johann Bauersachs,Stefan Engelhardt,Stefan Engelhardt +23 more