Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
TLDR
Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.Abstract:
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.read more
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Management of Diabetes in Coronavirus Disease 2019: Prognosis and Practical Issues
TL;DR: It is also important to note that SARS-CoV-2 may exacerbate glucose metabolism through direct beta-cell damage, leading to acute hyperglycemic complications and potential new onset diabetes.
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Analysis of Interaction Between Odorant Receptors and Flexible Spike of SARS CoV-2- Key to Loss of Smell
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Dynamics and self-assembly of the SARS-CoV-2 spike transmembrane domain
TL;DR: In this paper, the transmembrane structure of the SARS-CoV-2 spike was investigated using multiscale molecular dynamics simulations and the results provided by the results from the multispectral simulations not only provide insight into understudied trimeric helical interfaces in biomembranes, but also enhance our understanding of the elusive transmem-brane conformational dynamics of SARS and more generally of viral fusion proteins.
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Toward Atomistic Models of Intact SARS-CoV-2 via Martini Coarse-Grained Molecular Dynamics Simulations
TL;DR: In this paper , the authors constructed multiscale models of SARS-CoV-2 by integrating experimental data, structural modeling, and coarse-grained molecular dynamics simulations.
Posted ContentDOI
Allosteric perspective on the mutability and druggability of the SARS-CoV-2 Spike protein
TL;DR: In this paper, the structure and dynamics of the SARS-CoV-2 Spike protein were investigated and allosteric effects of mutations were considered in predictions and diagnostics of emerging variants of concern (VOCs).
References
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