Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
TLDR
Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.Abstract:
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.read more
Citations
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The dawn of mRNA vaccines: The COVID-19 case.
TL;DR: In less than one year since the outbreak of the COVID-19 pandemic, two mRNA-based vaccines, BNT162b2 and mRNA-1273, were granted the first historic authorization for emergency use, while another mRNA vaccine, CVnCoV, progressed to phase 3 clinical testing as mentioned in this paper.
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Distinguishing features of current COVID-19 vaccines: knowns and unknowns of antigen presentation and modes of action
Franz X. Heinz,Karin Stiasny +1 more
TL;DR: In this paper, the relevance of structural modifications of S in different vaccines and the different modes of antigen expression after vaccination with genetic adenovirus-vector and mRNA vaccines are reviewed.
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Broad betacoronavirus neutralization by a stem helix-specific human antibody.
Dora Pinto,Dora Pinto,Maximilian M. Sauer,Nadine Czudnochowski,Jun Siong Low,Jun Siong Low,M. Alejandra Tortorici,Michael P. Housley,Julia Noack,Alexandra C. Walls,John E. Bowen,Barbara Guarino,Barbara Guarino,Laura E. Rosen,Julia di Iulio,Josipa Jerak,Josipa Jerak,Josipa Jerak,Hannah Kaiser,Saiful Islam,Stefano Jaconi,Stefano Jaconi,Nicole Sprugasci,Nicole Sprugasci,Katja Culap,Katja Culap,Rana Abdelnabi,Caroline S. Foo,Lotte Coelmont,Istvan Bartha,Istvan Bartha,Siro Bianchi,Siro Bianchi,Chiara Silacci-Fregni,Chiara Silacci-Fregni,Jessica Bassi,Roberta Marzi,Roberta Marzi,Eneida Vetti,Eneida Vetti,Antonino Cassotta,Antonino Cassotta,Alessandro Ceschi,Paolo Ferrari,Pietro E. Cippà,Olivier Giannini,Samuele Ceruti,Christian Garzoni,Agostino Riva,Fabio Benigni,Fabio Benigni,Elisabetta Cameroni,Elisabetta Cameroni,Luca Piccoli,Luca Piccoli,Matteo Samuele Pizzuto,Matteo Samuele Pizzuto,Megan Smithey,David I. Hong,Amalio Telenti,Florian A. Lempp,Johan Neyts,Colin Havenar-Daughton,Antonio Lanzavecchia,Antonio Lanzavecchia,Federica Sallusto,Federica Sallusto,Gyorgy Snell,Herbert W. Virgin,Martina Beltramello,Davide Corti,David Veesler +71 more
TL;DR: In this article, the authors describe five monoclonal antibodies cross-reacting with the stem helix of multiple β-coronavirus spike glycoproteins isolated from COVID-19 convalescent individuals.
Journal ArticleDOI
Structural basis for enhanced infectivity and immune evasion of SARS-CoV-2 variants.
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Christy L. Lavine,Shaun Rawson,Hanqin Peng,Haisun Zhu,Krishna Anand,Pei Tong,Avneesh Gautam,Shen Lu,Sarah M. Sterling,Richard M. Walsh,Sophia Rits-Volloch,Jianming Lu,Duane R. Wesemann,Wei Yang,Michael S. Seaman,Bing Chen,Bing Chen +22 more
TL;DR: In this article, the full-length spike (S) trimers of the B.1.7 and B.351 variants of SARS-CoV-2 have been analyzed.
Journal ArticleDOI
Molecular mechanism of interaction between SARS-CoV-2 and host cells and interventional therapy.
TL;DR: In this article, the authors reviewed the current knowledge and crucial information about how SARS-CoV-2 attaches on the surface of host cells through a variety of receptors, such as ACE2, neuropilin-1, AXL, and antibody-FcγR complexes.
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