Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
TLDR
Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.Abstract:
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.read more
Citations
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Predicting Epitope Candidates for SARS-CoV-2
TL;DR: In this article , known epitope sequences from SARS, CoV, SARS-CoV-2, and other Coronaviridae were leveraged to identify additional antigen regions in 62K SARS CoV 2 genomes.
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Dissecting Functional Biological Interactions Using Modular RNA Nanoparticles
TL;DR: In this article , the authors explore the practical applications of NANPs in laboratory and clinical settings and discuss how we can use established nucleic acid research techniques to design effective NANP.
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The Local Topological Free Energy of the SARS-CoV-2 Spike Protein
TL;DR: In this article , the authors used a mathematical method to characterize the local topology/geometry of the SARS-CoV-2 Spike protein backbone and found that local conformational changes in the FP, HR1, and CH domains are associated with global conformational change in the RBD domain.
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Biparatopic nanobodies targeting the receptor binding domain efficiently neutralize SARS-CoV-2
Phillip Pymm,Samuel J. Redmond,Olan Dolezal,Francesca L Mordant,Ester Lopez,James P Cooney,Kathryn C. Davidson,Ebene R. Haycroft,Chee Wah Tan,Rebecca Seneviratna,Samantha Grimley,Damian F. J. Purcell,Stephen J. Kent,Adam K. Wheatley,Lin-Fa Wang,Andrew Leis,Alisa Glukhova,Maria Pellegrini,Amy W. Chung,Kanta Subbarao,Adam P Uldrich,Wai-Hong Tham,Dale I. Godfrey,Nicholas A Gherardin +23 more
TL;DR: In this article , the authors used yeast display of a synthetic nanobody library to isolate nanobodies that bind the receptor-binding domain (RBD) of SARS-CoV-2 and neutralize the virus.
Journal ArticleDOI
SARS-CoV-2 Protein S Fusion Peptide Is Capable of Wrapping Negatively-Charged Phospholipids
TL;DR: In this paper , all-atom molecular dynamics was used to analyse the binding of SARS-CoV-2 fusion peptide to specific phospholipids in model membranes composed of only one phospholine plus cholesterol in the presence of either Na+ or Ca2+.
References
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