Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
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TLDR
Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.Abstract:
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.read more
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Uncovering the structural flexibility of SARS-CoV-2 glycoprotein spike variants
Hiam R. S. Arruda,Tulio M. Lima,Renata G. F. Alvim,Fernanda B. A. Victorio,D. P. B. Abreu,Federico F. Marsili,Karen D. Cruz,Patricia Sosa-Acosta,Mauricio Quiñones-Vega,Jessica De Siqueira Guedes,Fábio C. S. Nogueira,Jerson L. Silva,Leda R. Castilho,Guilherme A. P. de Oliveira +13 more
TL;DR: Comparison of biochemical data of the glycosylated full-length ancestral and D614G spike together with three other highly transmissible strains classified by the World Health Organization as variants of concern (VOC): beta, gamma, and delta is presented.
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Targeted photodynamic neutralization of SARS-CoV-2 mediated by singlet oxygen
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Adaptive variations in SARS-CoV-2 spike proteins: effects on distinct virus-cell entry stages
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TL;DR: In this article , the authors compared the functions of ancestral D614G and Omicron VOCs using cell-free assays that can reveal differences in several distinct steps of the S-directed virus entry process.
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Landscape-Based Mutational Sensitivity Cartography and Network Community Analysis of the SARS-CoV-2 Spike Protein Structures: Quantifying Functional Effects of the Circulating Variants
TL;DR: In this paper, an integrative computational approach was used to examine molecular mechanisms underlying functional effects of the D614G mutation by exploring atomistic modeling of the SARS-CoV-2 spike proteins as allosteric regulatory machines.
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SARS-CoV-2 Spike-Mediated Entry and Its Regulation by Host Innate Immunity
TL;DR: In this article , the authors summarized the conserved features of spike-mediated viral entry in various SARS-CoV-2 VOC and highlighted the converging proteolytic processes involved in priming and activating the spike.
References
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