Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
TLDR
Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.Abstract:
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.read more
Citations
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Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme
TL;DR: In this paper , the authors report on sample IS/17575 since it generated highly divergent results in the Belgian SARS-CoV-2 serology external quality assessment scheme.
Posted ContentDOI
Mathematical Analysis and Topology of SARS-CoV-2, Bonding with Cells and Unbonding
TL;DR: In this paper, the structure of the SARS-Cov-2 was analyzed in terms of the number of spikes that are critical in bonding with the cells in the host.
Journal ArticleDOI
Comparative analysis of candidate vaccines to prevent covid 19 pandemic
Radha Yadav,Lalita Chopra +1 more
TL;DR: In this paper, the potential factors that are required when creating vaccines as well as a comparative analysis of data obtained from SARS-CoV-2 vaccine trials for different vaccines and environmental impacts of its preparation.
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Intramuscular administration of recombinant Newcastle disease virus expressing SARS-CoV-2 spike protein protects hACE-2 TG mice against SARS-CoV-2 infection
Deok-Hwan Kim,Ji-Hye Lee,Sungsu Youk,Jei-Hyun Jeong,Da-Ye Lee,Ha-Na Youn,Ji-Eun Park,Ji-Yun Kim,Tae-Hyeon Kim,Dong-Hun Lee,Pil-gu Park,Kee-jong Hong,Chang-Seon Song +12 more
TL;DR: In this article , a recombinant NDV-vectored vaccine expressing the spike (S) protein of SARS-CoV-2, rK148/beta-S, was developed and evaluated for its efficacy against SARS CoV2 in K18-hACE-2 transgenic mice.
Posted ContentDOI
Entropic overcompensation of the N501Y mutation on SARS-CoV-2 S binding to ACE2
TL;DR: These findings suggest that an important factor in determining protein-protein binding affinity is the degree to which fluctuations are distributed throughout the complex, and that residue mutations that may seem to result in weaker interactions than their wild-type counterparts may yet result increased binding affinity thanks to their ability to suppress unfavorable entropy changes upon binding.
References
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