Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
TLDR
Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.Abstract:
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.read more
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Journal ArticleDOI
Structures of SARS-CoV-2 spike protein alert noteworthy sites for the potential approaching variants
Xiaorui Xing,Zhen Cui,Wangjun Fu,Tao Zheng,Lili Qin,Pingju Ge,Ai-Hua Qian,Nianshuang Wang,Shuai Yuan +8 more
TL;DR: The authors showed that deletion of residues 156-157 warps the neighboring beta-sheet and leads NTD and RBD to shift, and T859N stabilizes the packing of the 630 loop motif to make RBD standing transition more difficult.
Book ChapterDOI
Biotechnology focuses on fast-moving consumer goods, pharmaceutical personal care products, medicines, and foods
TL;DR: In this article , the authors highlight the most relevant aspects of the development of FMCG, PPCP, pharmaceuticals, and foods, addressed currently by the biotechnological industry.
Posted ContentDOI
The local topological free energy of the SARS-CoV-2 Spike protein
TL;DR: In this article, the authors used density functional theory (DFT) calculations with inclusion of solvent effects to associate a local topological/geometrical free energy along the SARS-CoV-2 spike protein backbone.
Journal ArticleDOI
TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry
Jim Baggen,Maarten Jacquemyn,Leentje Persoons,E. Vanstreels,Valerie E. Pye,A.G. Wrobel,Valeria Calvaresi,Stephen R. Martin,Chloe Roustan,Nora Cronin,Eamonn Reading,Hendrik Jan Thibaut,Thomas Vercruysse,Piet Maes,Frederik De Smet,Angie Yee,Toey Nivitchanyong,Marina Roell,Herve Rhinn,Alusha A. Mamchak,Eric J. Brown,Peter Cherepanov,Dirk Daelemans +22 more
TL;DR: Yang et al. as mentioned in this paper showed that TMEM106B, a lysosomal transmembrane protein, can serve as an alternative receptor for SARS-CoV-2 entry into angiotensin-converting enzyme 2 (ACE2)-negative cells.
Journal ArticleDOI
Unsupervised Cryo-EM Images Denoising and Clustering Based on Deep Convolutional Autoencoder and K-Means++
TL;DR: Wang et al. as mentioned in this paper proposed an iterative denoising and clustering method based on a deep convolutional variational autoencoder and K-means++.
References
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Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
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TL;DR: PHENIX has been developed to provide a comprehensive system for macromolecular crystallographic structure solution with an emphasis on the automation of all procedures.
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