Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
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TLDR
Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.Abstract:
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.read more
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Preclinical evaluation of a SARS-CoV-2 mRNA vaccine PTX-COVID19-B
Eric G. Marcusson,Reuben Samson,Patrick Budylowski,Matías Andrés Bellalta Bremer,Paula Arribas +4 more
TL;DR: A lipid nanoparticle-formulated SARS-CoV-2 mRNA vaccine, PTX-COVID19-B, was chosen among three candidates after the initial mouse vaccination results showed that it elicited the strongest neutralizing antibody response against SARS CoV2 as discussed by the authors .
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Transient lipid-bound states of spike protein heptad repeats provide insights into SARS-CoV-2 membrane fusion.
TL;DR: The entry of SARS-CoV-2 into a host cell is mediated by spike, a class I viral fusion protein responsible for merging the viral and host cell membranes as mentioned in this paper.
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Emergence of a recurrent insertion in the N-terminal domain of the SARS-CoV-2 spike glycoprotein
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Structural Plasticity and Immune Evasion of SARS-CoV-2 Spike Variants
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TL;DR: Recent findings on S-associated virus transmissibility and immune evasion of SARS-CoV-2 VOCs and experimental approaches used to profile these properties are discussed.
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References
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