Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
TLDR
Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.Abstract:
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.read more
Citations
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Dynamic Characteristic Analysis of Antibodies in Patients With COVID-19: A 13-Month Study.
Danrong Shi,Tianhao Weng,Jie Wu,Chunyan Dai,Rui Luo,Keda Chen,Miaojin Zhu,Xiangyun Lu,Linfang Cheng,Qiuqiang Chen,Fumin Liu,Zhigang Wu,Haibo Wu,Changzhong Jin,Miao Guo,Zhe Chen,Nanping Wu,Hangping Yao,Min Zheng +18 more
TL;DR: In this paper, the authors described the long traceable antibody response of the COVID-19 and offered hints about targets to screen for postinfectious immunity and for vaccination development of SARS-CoV-2.
Journal ArticleDOI
Global Genomic Analysis of SARS-CoV-2 RNA Dependent RNA Polymerase Evolution and Antiviral Drug Resistance
Alfredo Mari,Alfredo Mari,Alfredo Mari,Tim Roloff,Tim Roloff,Tim Roloff,Madlen Stange,Madlen Stange,Madlen Stange,Kirstine K. Søgaard,Kirstine K. Søgaard,Erblin Asllanaj,Erblin Asllanaj,Gerardo Tauriello,Gerardo Tauriello,Leila Tamara Alexander,Leila Tamara Alexander,Michael Schweitzer,Michael Schweitzer,Karoline Leuzinger,Karoline Leuzinger,Alexander Gensch,Aurélien Emmanuel Martinez,Julia Bielicki,Hans Pargger,Martin Siegemund,Christian H. Nickel,Roland Bingisser,Michael Osthoff,Stefano Bassetti,Parham Sendi,Parham Sendi,Manuel Battegay,Catia Marzolini,Helena M. B. Seth-Smith,Helena M. B. Seth-Smith,Helena M. B. Seth-Smith,Torsten Schwede,Torsten Schwede,Hans H. Hirsch,Hans H. Hirsch,Adrian Egli,Adrian Egli +42 more
TL;DR: In this paper, the authors proposed a framework to monitor the emergence of antiviral resistance, and as a proof of concept, they addressed the interaction between RdRp and remdesivir.
Posted ContentDOI
The SARS-CoV-2 Spike harbours a lipid binding pocket which modulates stability of the prefusion trimer
Loic Carrique,Helen M. E. Duyvesteyn,Tomas Malinauskas,Yuguang Zhao,Jingshan Ren,D. Zhou,Thomas S. Walter,Julika Radecke,Jiandong Huo,Jiandong Huo,Reinis R. Ruza,Pranav N.M. Shah,Elizabeth E. Fry,David I. Stuart +13 more
TL;DR: SARS-CoV-2 Spike can adopt a ‘locked’ conformation with all receptor binding domains (RBDs) down, likely to represent the prefusion resting state, revealing a potentially druggable pocket and suggesting that the natural prefusion state occludes neutralising RBD epitopes, achieving conformational shielding from antibodies.
Journal ArticleDOI
A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19
Delphine C. Malherbe,Drishya Kurup,Christoph Wirblich,Adam J. Ronk,Chad E. Mire,Natalia Kuzmina,Noor F. Shaik,Sivakumar Periasamy,Matthew A. Hyde,Julie M. Williams,Pei Yong Shi,Matthias J. Schnell,Alexander Bukreyev +12 more
TL;DR: In this article, a replication-competent vesicular stomatitis virus (VSV) vaccine vector that expresses the S1 subunit of SARS-CoV-2 spike protein was designed and produced.
Journal ArticleDOI
Advances of nanomaterials-based strategies for fighting against COVID-19
TL;DR: Nanomaterials facilitate the development of easy, fast, and low‐cost diagnostic assays to detect SARS‐CoV‐2 and related biomarkers and enable the efficient delivery of viral antigens to antigen‐presenting cells or serve as adjuvants in the host, leading to vaccine development at an unprecedented pace.
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