Distinct conformational states of SARS-CoV-2 spike protein
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Shaun Rawson,Sophia Rits-Volloch,Bing Chen,Bing Chen +12 more
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TLDR
Two cryo–electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion and postfusion conformations, are reported, advancing the understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.Abstract:
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.read more
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Self-derived peptides from the SARS-CoV-2 spike glycoprotein disrupting shaping and stability of the homotrimer unit
TL;DR: In this article , the structure-based design approach was used to develop self-derived S peptides, which could replace a single S protein monomer, blocking the homotrimer formation or inducing stability.
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Recent SARS-CoV-2 Outlook and Implications in a COVID-19 Vaccination Era
TL;DR: In this article, the role of the viral glycocalyx in modulating the susceptibility and severity of infection through attraction and repulsive interactions has been investigated in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
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Interspecies transmission of SARS CoV-2 with special emphasis on viral mutations and ACE-2 receptor homology roles
Mervat E. Hamdy,Ayman H. El Deeb,Naglaa Hagag,Momtaz A. Shahein,Osama Alaidi,Hussein A. Hussein +5 more
TL;DR: In this article , structural aspects of SARS-CoV-2 spike protein, the effect of different mutations observed in the spike protein and the impact of ACE2 receptor variations in different animal hosts on inter-species transmission were reviewed.
Posted ContentDOI
Evolutionary and phenotypic characterization of spike mutations in a new SARS-CoV-2 Lineage reveals two Variants of Interest
Paula Ruiz-Rodríguez,Clara Francés-Gómez,Álvaro Chiner-Oms,Mariana G. López,Santiago Jimenez-Serrano,Irving Cancino-Muñoz,Paula Ruiz-Hueso,Manuela Torres-Puente,María Alma Bracho,Giuseppe D'Auria,Martinez-Priego L,Manuel Guerreiro,Montero-Alonso M,Gómez,José Luis Piñana,Fernando González-Candelas,Iñaki Comas,Alberto Marina,Ron Geller,Mireia Coscolla +19 more
TL;DR: In this article, the authors presented a study that was funded by the European Union through the Operational Program of the European Regional Development Fund (ERDF) of the Valencian Community 2014-2020.
Posted ContentDOI
Why are SNAREpins rod-shaped?
TL;DR: In this paper, the authors used ultra-coarse-grained simulations to investigate the minimal requirements for a molecular intracellular fusogen, and to elucidate the mechanisms of SNARE-mediated fusion.
References
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