Journal ArticleDOI
Expression of a Cu,Zn superoxide dismutase typical of familial amyotrophic lateral sclerosis induces mitochondrial alteration and increase of cytosolic Ca2+ concentration in transfected neuroblastoma SH-SY5Y cells
Maria Teresa Carrì,Alberto Ferri,Andrea Battistoni,Laila Famhy,Roberta Gabbianelli,Fabrizio Poccia,Giuseppe Rotilio +6 more
TLDR
It is reported that G93A induces a significant loss of mitochondrial membrane potential, an increased sensitivity toward valinomycin and a parallel increase in cytosolic Ca2+ concentration, which are not related to total Cu,ZnSOD content and activity in the cell.About:
This article is published in FEBS Letters.The article was published on 1997-09-08. It has received 234 citations till now. The article focuses on the topics: Superoxide dismutase & SH-SY5Y.read more
Citations
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Mitochondrial control of cell death
Guido Kroemer,John C. Reed +1 more
TL;DR: In many instances, permeabilization of mitochondrial membranes is a rate-limiting step of apoptotic or necrotic cell demise, which has important consequences for the pathophysiology of cell death, as well as for its pharmacological control.
Journal ArticleDOI
Massive Mitochondrial Degeneration in Motor Neurons Triggers the Onset of Amyotrophic Lateral Sclerosis in Mice Expressing a Mutant SOD1
Jiming Kong,Zuoshang Xu +1 more
TL;DR: The absence of massive motor neuron death at the early stages of the disease indicates that the majority of motor neurons could be rescued after clinical diagnosis, and indicates that mutant SOD1 toxicity is mediated by damage to mitochondria in motor neurons.
Journal ArticleDOI
Neuroprotective effects of creatine in a transgenic animal model of amyotrophic lateral sclerosis
Péter Klivényi,Robert J. Ferrante,Robert J. Ferrante,Russell T. Matthews,Mikhail B. Bogdanov,Autumn M. Klein,Autumn M. Klein,Ole A. Andreassen,Gerald Mueller,Marike Wermer,Rima Kaddurah-Daouk,M F Beal,M F Beal +12 more
TL;DR: It was found that oral administration of creatine produced a dose-dependent improvement in motor performance and extended survival in G93A transgenic mice, and it protected mice from loss of both motor neurons and substantia nigra neurons at 120 days of age.
Journal ArticleDOI
Mitochondrial Dysfunction in Neurodegenerative Diseases
Ashu Johri,M. Flint Beal +1 more
TL;DR: A concise overview of the major findings in recent years highlighting the importance of healthy mitochondria for a healthy neuron in neurodegenerative diseases is provided.
Journal ArticleDOI
Mitochondrial dysfunction in neurodegenerative diseases
TL;DR: A potential pivotal role for mitochondrial dysfunction in neurodegenerative diseases is gaining increasing acceptance and therapeutic approaches include methods to buffer intracellular ATP and to scavenge free radicals.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels☆
TL;DR: The staining procedure for localizing superoxide dismutase on polyacrylamide electrophoretograms has been applied to extracts obtained from a variety of sources and could thus be assayed either in crude extracts or in purified protein fractions.